High-Fidelity PCR Kits

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PCR Master Mixes and Kits

Overview

iProof™ Polymerase Benefits

Comparison of common thermostable DNA polymerases. iProof Polymerase exhibits the highest fidelity of any commercially available thermostable polymerase, >50-fold higher than ordinary Taq polymerase. A common high-fidelity polymerase was ~8-fold higher than Taq, and an ultrahigh-fidelity polymerase was ~32-fold higher.

iProof Polymerase demonstrates unrivaled speed, leading to dramatically shorter overall reaction times. The reaction protocol for iProof Polymerase was compared to the recommended protocols for two competing polymerases. Each protocol was designed to amplify 1, 8, or 15 kb products in 30 cycles. Reactions with iProof Polymerase used a two-step protocol with a combined annealing and extension step, while the other reactions used three-step protocols with the minimum recommended extension times. Overall reaction times include temperature ramping times.

iProof™ Polymerase Performance Data

Comparison of amplification using iProof Polymerase and another supplier's fast- cycling enzyme. Manufacturer's recommended cycling conditions were used for both enzymes. Left, a 1 kb fragment was amplified from 10 ng human genomic DNA using 20 U/ml of iProof Polymerase (lanes 6–9) or 50 U/ml of the other enzyme (lanes 20–24) in a standard-ramping thermal cycler. Total run time for protocols using a 30 sec annealing/extension step was 48 min (lanes 6, 7, 20, and 21). Total run time for protocols using a 15 sec annealing/extension step was 39 min (lanes 8 and 9) or 40 min (lanes 22 and 23). Lane 5, 500 bp EZ Load marker. Right, the same 1 kb fragment was amplified from 100 ng human genomic DNA on a fast-ramping thermal cycler, using 50 U/ml of iProof Polymerase (lane 3) or 100 U/ml of the other enzyme (lane 4), and using an annealing/extension step of 30 sec. Total run time on the fast ramping cycler was 30 min. Lane 2, 500 bp EZ Load Marker.

Comparison of amplification using iProof Polymerase and another supplier's fast- cycling enzyme. Manufacturer's recommended cycling conditions were used for both enzymes. Left, a 1 kb fragment was amplified from 10 ng human genomic DNA using 20 U/ml of iProof Polymerase (lanes 6–9) or 50 U/ml of the other enzyme (lanes 20–24) in a standard-ramping thermal cycler. Total run time for protocols using a 30 sec annealing/extension step was 48 min (lanes 6, 7, 20, and 21). Total run time for protocols using a 15 sec annealing/extension step was 39 min (lanes 8 and 9) or 40 min (lanes 22 and 23). Lane 5, 500 bp EZ Load marker. Right, the same 1 kb fragment was amplified from 100 ng human genomic DNA on a fast-ramping thermal cycler, using 50 U/ml of iProof Polymerase (lane 3) or 100 U/ml of the other enzyme (lane 4), and using an annealing/extension step of 30 sec. Total run time on the fast ramping cycler was 30 min. Lane 2, 500 bp EZ Load Marker.

iProof Polymerase demonstrates increased speed with minimal amounts of enzyme. The gel images below compare the performance of three thermostable polymerases in amplifying a 1 kb DNA fragment using a two-step protocol that combined annealing and extension times ranging from 7 to 240 sec. iProof Polymerase successfully amplified the target with the shortest extension time (15 sec). Competing enzymes required an annealing/extension step of 30–60 sec or more and 5- to 10-fold more enzyme to produce comparable yields.

Annealing/extension time, sec
iProof High-Fidelity Polymerase Competitor High-Fidelity Polymerase Taq Polymerase

iProof DNA Polymerase delivers high yields with significantly less enzyme. A 1 kb fragment was amplified from 1.25 pM of lambda DNA template in 20 cycles, using combined annealing/extension step of variable duration as indicated. All enzymes were used according to manufacturer recommendations.

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iProof™ High-Fidelity DNA Polymerase is a unique Pyrococcus-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.

Features and Benefits of iProof High-Fidelity DNA Polymerase:

  • High fidelity — iProof DNA polymerase is 52-fold more accurate than Taq polymerase
  • Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
  • Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)

Choose the best DNA polymerase for your application.

Key Features iProof DNA Polymerase iTaq DNA Polymerase
Speed

Processivity

Long fragment amplification

Proofreading Yes No
Hot-Start No Yes
 

Related Categories

Supporting Documentation

Storage at –20°C iProof™ Enzyme, Kit, and Buffers: guaranteed for 6 months at –20°C in a constant-temperature freezer
iProof Master Mixes: guaranteed for 3 months at –20°C in a constant-temperature freezer (multiple freeze/thaws not recommended)
Storage at +4°C Guaranteed for 3 months at 4°C
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iProof™ High-Fidelity PCR Kit, 50 U (2 U/µl), 25 µl

1725330
50 U, 2 U/µl, iProof polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard

1725330 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5330_0921120544437.xml 1725330 09/21/09 12:05 PM en_US true internet/product_SKU 2018-03-15 23:40:03 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5330_0921120544437.xml /default/main/bio-rad/WORKAREA/default jcrucyed 2017-11-14 07:07:40 true jcrucyed templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5330_0921120544437.xml LSR N /default/main/bio-rad en 09/21/19 12:05 PM 1520306495944 2018-03-15 23:40:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5330_0921120544437.xml 1725330-iproof-high-fidelity-pcr-kit-50-u-2-u-ul-25-ul 1 1725330 iProof High-Fidelity PCR Kit iProof™ High-Fidelity PCR Kit, 50 U (2 U/µl), 25 µl 50 U, 2 U/&#181;l, iProof polymerase PCR kit includes 5x reaction buffers, MgCl<sub>2</sub> solution, DMSO, dNTPs, &lambda; DNA, 1.3 and 10 kb primers, DNA standard 50 U, 2 U/&#181;l, iProof polymerase PCR kit includes 5x reaction buffers, MgCl<sub>2</sub> solution, DMSO, dNTPs, &lambda; DNA, 1.3 and 10 kb primers, DNA standard 50 U, 2 U/&#181;l, iProof polymerase PCR kit includes 5x reaction buffers, MgCl<sub>2</sub> solution, DMSO, dNTPs, &lambda; DNA, 1.3 and 10 kb primers, DNA standard /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5330_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5330_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/172-5330_feature.jpg RepEx 172-5330 50 U, 2 U/&#181;l, iProof polymerase PCR kit includes 5x reaction buffers, MgCl<sub>2</sub> solution, DMSO, dNTPs, &lambda; DNA, 1.3 and 10 kb primers, DNA standard 1725330, iproof, high-fidelity, pcr, reagents, polymerase, chain, reaction, high, fidelity, buffers, mgcl2, dmso, dntps, lambda, dna, primers, standard, detection, amplification, thermal, cycling 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/Thermal-Cyclers N 0 <p>iProof High-Fidelity DNA Polymerase is a unique <em>Pyrococcus</em>-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.</p> <h2 class="descHeader">Features and Benefits of iProof High-Fidelity DNA Polymerase:</h2> <ul> <li><strong>High fidelity</strong> &mdash; iProof DNA polymerase is 52-fold more accurate than <em>Taq</em> polymerase</li> <li><strong>Speed</strong> &mdash; high processivity dramatically reduces extension (15&ndash;30 sec/kb) and overall reaction times</li> <li><strong>Successful amplification of long products with higher yields</strong> &mdash; fragments up to 37 kb are amplified in less time and with less enzyme (0.25&ndash;1 unit/reaction)</li> </ul> <p>iProof Polymerase is available in an easy-to-use master mix and a PCR kit complete with controls.</p> <table class="pd_table pd_gridlines" style="text-align: left;" border="0"> <tbody> <tr> <td valign="top"><strong>iProof High-Fidelity DNA Polymerase</strong></td> <td align="center" valign="top"><strong>Number of Reactions</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity PCR Kit</td> <td align="center">50 U (2 U/&micro;l)</td> <td align="center"><span style="font-size: 11px;">1725330</span></td> </tr> <tr> <td>iProof High-Fidelity PCR Kit</td> <td align="center">200 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725331-iproof-high-fidelity-pcr-kit-200-u-2-u-ul-100-ul?ID=1725331">1725331</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof HF Master Mix</td> <td align="center">100 x 50 &micro;l reactions</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725310-iproof-hf-master-mix-100-x-50-ul-rxns-2-5-ml?ID=1725310">1725310</a></td> </tr> <tr> <td>iProof GC Master Mix</td> <td align="center">100 x 50 &micro;l reactions</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725320-iproof-gc-master-mix-100-x-50-ul-rxns-2-5-ml?ID=1725320">1725320</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">20 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725300-iproof-high-fidelity-dna-polymerase-20-u-2-u-ul-10-ul">1725300</a></td> </tr> <tr> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">100 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725301-iproof-high-fidelity-dna-polymerase-100-u-2-u-ul-50-ul">1725301</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">500 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725302-iproof-high-fidelity-dna-polymerase-500-u-2-u-ul-250-ul">1725302</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Categories</h2> <ul> <ul> <li><a href="/en-us/category/high-fidelity-standard-pcr-reagents">Standard PCR Enzymes and Reagents</a></li> <li><a href="/en-us/category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA Isolation Kits</a></li> <li><a href="/en-us/category/cdna-synthesis-kits?ID=M87EPGE8Z">Reverse Transcription Kits</a></li> <li><a href="/en-us/category/real-time-pcr-supermixes-kits?ID=M87F1J8UU">Real-time PCR Supermixes </a></li> </ul> </ul> <div id="wetLab"> <script type="text/javascript">// <![CDATA[ $('#wetLab').load('/webroot/web/html/lsr/products/amplification_pcr/wet-lab.html'); // ]]></script> </div> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>iProof High-Fidelity PCR Kit <a href="/webroot/web/pdf/lsr/literature/10002300B.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>iProof High-Fidelity Master Mix <a href="/webroot/web/pdf/lsr/literature/10002299.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer">Brochure</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global iproof-high-fidelity-dna-polymerase-kit-1725330-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/iproof-high-fidelity-dna-polymerase-kit-1725330-desc.html 1708842 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8842_1301949358.xml 1708841 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725330&CartQuote=QT&quantity= false true false $79.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725330&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725330&quantity= 1725330
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iProof™ High-Fidelity PCR Kit, 200 U (2 U/µl), 100 µl

1725331
200 U, 2 U/µl, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard

1725331 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5331_0921120544258.xml 1725331 09/21/09 12:05 PM en_US true internet/product_SKU 2018-03-15 23:40:06 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5331_0921120544258.xml /default/main/bio-rad/WORKAREA/default jcrucyg6 2017-11-14 07:07:40 true jcrucyg6 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5331_0921120544258.xml LSR N /default/main/bio-rad en 09/21/19 12:05 PM 1520306495990 2018-03-15 23:40:06 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5331_0921120544258.xml 1725331-iproof-high-fidelity-pcr-kit-200-u-2-u-ul-100-ul 1 1725331 iProof High-Fidelity PCR Kit iProof™ High-Fidelity PCR Kit, 200 U (2 U/µl), 100 µl 200 U, 2 U/&#181;l, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl<sub>2</sub> solution, DMSO, dNTPs, &lambda; DNA, 1.3 and 10 kb primers, DNA standard 200 U, 2 U/&#181;l, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl<sub>2</sub> solution, DMSO, dNTPs, &lambda; DNA, 1.3 and 10 kb primers, DNA standard 200 U, 2 U/&#181;l, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl<sub>2</sub> solution, DMSO, dNTPs, &lambda; DNA, 1.3 and 10 kb primers, DNA standard /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5331_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5331_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/172-5331_feature.jpg RepEx 172-5331 200 U, 2 U/&#181;l, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl<sub>2</sub> solution, DMSO, dNTPs, &lambda; DNA, 1.3 and 10 kb primers, DNA standard 1725331, iproof, high-fidelity, pcr, reagents, polymerase, chain, reaction, high, fidelity, buffers, mgcl2, dmso, dntps, lambda, dna, primers, standard, detection, amplification, thermal, cycling 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/Thermal-Cyclers N 0 <p>iProof High-Fidelity DNA Polymerase is a unique <em>Pyrococcus</em>-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.</p> <h2 class="descHeader">Features and Benefits of iProof High-Fidelity DNA Polymerase:</h2> <ul> <li><strong>High fidelity</strong> &mdash; iProof DNA polymerase is 52-fold more accurate than <em>Taq</em> polymerase</li> <li><strong>Speed</strong> &mdash; high processivity dramatically reduces extension (15&ndash;30 sec/kb) and overall reaction times</li> <li><strong>Successful amplification of long products with higher yields</strong> &mdash; fragments up to 37 kb are amplified in less time and with less enzyme (0.25&ndash;1 unit/reaction)</li> </ul> <p>iProof Polymerase is available in an easy-to-use master mix and a PCR kit complete with controls.</p> <table class="pd_table pd_gridlines" style="text-align: left;" border="0"> <tbody> <tr> <td valign="top"><strong>iProof High-Fidelity DNA Polymerase</strong></td> <td align="center" valign="top"><strong>Number of Reactions</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity PCR Kit</td> <td align="center">50 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725330-iproof-high-fidelity-pcr-kit-50-u-2-u-ul-25-ul">1725330</a></td> </tr> <tr> <td>iProof High-Fidelity PCR Kit</td> <td align="center">200 U (2 U/&micro;l)</td> <td align="center"><span style="font-size: 11px;">1725331</span></td> </tr> <tr class="pd_colorbackground"> <td>iProof HF Master Mix</td> <td align="center">100 x 50 &micro;l reactions</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725310-iproof-hf-master-mix-100-x-50-ul-rxns-2-5-ml?ID=1725310">1725310</a></td> </tr> <tr> <td>iProof GC Master Mix</td> <td align="center">100 x 50 &micro;l reactions</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725320-iproof-gc-master-mix-100-x-50-ul-rxns-2-5-ml?ID=1725320">1725320</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">20 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725300-iproof-high-fidelity-dna-polymerase-20-u-2-u-ul-10-ul">1725300</a></td> </tr> <tr> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">100 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725301-iproof-high-fidelity-dna-polymerase-100-u-2-u-ul-50-ul">1725301</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">500 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725302-iproof-high-fidelity-dna-polymerase-500-u-2-u-ul-250-ul">1725302</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Categories</h2> <ul> <ul> <li><a href="/en-us/category/high-fidelity-standard-pcr-reagents">Standard PCR Enzymes and Reagents</a></li> <li><a href="/en-us/category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA Isolation Kits</a></li> <li><a href="/en-us/category/cdna-synthesis-kits?ID=M87EPGE8Z">Reverse Transcription Kits</a></li> <li><a href="/en-us/category/real-time-pcr-supermixes-kits?ID=M87F1J8UU">Real-time PCR Supermixes </a></li> </ul> </ul> <div id="wetLab"> <script type="text/javascript">// <![CDATA[ $('#wetLab').load('/webroot/web/html/lsr/products/amplification_pcr/wet-lab.html'); // ]]></script> </div> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>iProof High-Fidelity PCR Kit <a href="/webroot/web/pdf/lsr/literature/10002300B.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>iProof High-Fidelity Master Mix <a href="/webroot/web/pdf/lsr/literature/10002299.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer">Brochure</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global iproof-high-fidelity-dna-polymerase-kit-1725331-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/iproof-high-fidelity-dna-polymerase-kit-1725331-desc.html 1708841 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8841_1284066788.xml 1708842 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/170-8842_1301949358.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725331&CartQuote=QT&quantity= false true false $283.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725331&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725331&quantity= 1725331
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iProof™ HF Master Mix, 100 x 50 µl rxns, 2.5 ml

1725310
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates

1725310 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5310_0921120544328.xml 1725310 09/21/09 12:05 PM en_US true internet/product_SKU 2018-03-15 23:40:04 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5310_0921120544328.xml /default/main/bio-rad/WORKAREA/default jcrucyf0 2017-11-14 07:07:40 true jcrucyf0 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5310_0921120544328.xml LSR N /default/main/bio-rad en 09/21/19 12:05 PM 1520306495820 2018-03-15 23:40:04 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5310_0921120544328.xml 1725310-iproof-hf-master-mix-100-x-50-ul-rxns-2-5-ml 1 1725310 iProof HF Master Mix iProof™ HF Master Mix, 100 x 50 µl rxns, 2.5 ml 100 x 50 &#181;l reactions, premixed PCR reagents, includes 2x master mix (0.04 U/&#181;l), DMSO; suitable for most templates 100 x 50 &#181;l reactions, premixed PCR reagents, includes 2x master mix (0.04 U/&#181;l), DMSO; suitable for most templates 100 x 50 &#181;l reactions, premixed PCR reagents, includes 2x master mix (0.04 U/&#181;l), DMSO; suitable for most templates /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5310_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5310_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/172-5310_feature.jpg RepEx 172-5310 100 x 50 &#181;l reactions, premixed PCR reagents, includes 2x master mix (0.04 U/&#181;l), DMSO; suitable for most templates 172-5310, 1725310, iproof, hf, master, mix, pcr, polymerase, chain, reaction, reagent, high, fidelity, premix, amplification, high-fidelity, supermix, mixes 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/Thermal-Cyclers N 0 <p>iProof High-Fidelity DNA Polymerase is a unique <em>Pyrococcus</em>-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.</p> <h2 class="descHeader">Features and Benefits of iProof High-Fidelity DNA Polymerase:</h2> <ul> <li><strong>High fidelity</strong> &mdash; iProof DNA polymerase is 52-fold more accurate than <em>Taq</em> polymerase</li> <li><strong>Speed</strong> &mdash; high processivity dramatically reduces extension (15&ndash;30 sec/kb) and overall reaction times</li> <li><strong>Successful amplification of long products with higher yields</strong> &mdash; fragments up to 37 kb are amplified in less time and with less enzyme (0.25&ndash;1 unit/reaction)</li> </ul> <p>iProof Polymerase is available in an easy-to-use master mix and a PCR kit complete with controls.</p> <table class="pd_table pd_gridlines" style="text-align: left;" border="0"> <tbody> <tr> <td valign="top"><strong>iProof High-Fidelity DNA Polymerase</strong></td> <td align="center" valign="top"><strong>Number of Reactions</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity PCR Kit</td> <td align="center">50 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725330-iproof-high-fidelity-pcr-kit-50-u-2-u-ul-25-ul">1725330</a></td> </tr> <tr> <td>iProof High-Fidelity PCR Kit</td> <td align="center">200 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725331-iproof-high-fidelity-pcr-kit-200-u-2-u-ul-100-ul?ID=1725331">1725331</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof HF Master Mix</td> <td align="center">100 x 50 &micro;l reactions</td> <td align="center"><span style="font-size: 11px;">1725310</span></td> </tr> <tr> <td>iProof GC Master Mix</td> <td align="center">100 x 50 &micro;l reactions</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725320-iproof-gc-master-mix-100-x-50-ul-rxns-2-5-ml?ID=1725320">1725320</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">20 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725300-iproof-high-fidelity-dna-polymerase-20-u-2-u-ul-10-ul">1725300</a></td> </tr> <tr> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">100 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725301-iproof-high-fidelity-dna-polymerase-100-u-2-u-ul-50-ul">1725301</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">500 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725302-iproof-high-fidelity-dna-polymerase-500-u-2-u-ul-250-ul">1725302</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Categories</h2> <ul> <ul> <li><a href="/en-us/category/high-fidelity-standard-pcr-reagents">Standard PCR Enzymes and Reagents</a></li> <li><a href="/en-us/category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA Isolation Kits</a></li> <li><a href="/en-us/category/cdna-synthesis-kits?ID=M87EPGE8Z">Reverse Transcription Kits</a></li> <li><a href="/en-us/category/real-time-pcr-supermixes-kits?ID=M87F1J8UU">Real-time PCR Supermixes </a></li> </ul> </ul> <div id="wetLab"> <script type="text/javascript">// <![CDATA[ $('#wetLab').load('/webroot/web/html/lsr/products/amplification_pcr/wet-lab.html'); // ]]></script> </div> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>iProof High-Fidelity PCR Kit <a href="/webroot/web/pdf/lsr/literature/10002300B.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>iProof High-Fidelity Master Mix <a href="/webroot/web/pdf/lsr/literature/10002299.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer">Brochure</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global iproof-premixed-pcr-reagents-1725310-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/iproof-premixed-pcr-reagents-1725310-desc.html 1708352 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-8352_0921120432022.xml 1708890 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725310&CartQuote=QT&quantity= false true false $206.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725310&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725310&quantity= 1725310
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iProof™ GC Master Mix, 100 x 50 µl rxns, 2.5 ml

1725320
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for GC-rich templates

1725320 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5320_0921120544397.xml 1725320 09/21/09 12:05 PM en_US true internet/product_SKU 2018-03-15 23:40:05 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5320_0921120544397.xml /default/main/bio-rad/WORKAREA/default jcrucyg4 2017-11-14 07:07:40 true jcrucyg4 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5320_0921120544397.xml LSR N /default/main/bio-rad en 09/21/19 12:05 PM 1520306495876 2018-03-15 23:40:05 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Thermal-Cyclers/172-5320_0921120544397.xml 1725320-iproof-gc-master-mix-100-x-50-ul-rxns-2-5-ml 1 1725320 iProof GC Master Mix iProof™ GC Master Mix, 100 x 50 µl rxns, 2.5 ml 100 x 50 &#181;l reactions, premixed PCR reagents, includes 2x master mix (0.04 U/&#181;l), DMSO; suitable for GC-rich templates 100 x 50 &#181;l reactions, premixed PCR reagents, includes 2x master mix (0.04 U/&#181;l), DMSO; suitable for GC-rich templates 100 x 50 &#181;l reactions, premixed PCR reagents, includes 2x master mix (0.04 U/&#181;l), DMSO; suitable for GC-rich templates /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5320_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5320_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/172-5320_feature.jpg RepEx 172-5320 100 x 50 &#181;l reactions, premixed PCR reagents, includes 2x master mix (0.04 U/&#181;l), DMSO; suitable for GC-rich templates 172-5320, 1725320, iproof, gc, master, mix, pcr, polymerase, chain, reaction, reagent, premix, amplification, supermix, mixes, gc-rich, rich, templates, template 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/Thermal-Cyclers N 0 <p>iProof High-Fidelity DNA Polymerase is a unique <em>Pyrococcus</em>-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.</p> <h2 class="descHeader">Features and Benefits of iProof High-Fidelity DNA Polymerase:</h2> <ul> <li><strong>High fidelity</strong> &mdash; iProof DNA polymerase is 52-fold more accurate than <em>Taq</em> polymerase</li> <li><strong>Speed</strong> &mdash; high processivity dramatically reduces extension (15&ndash;30 sec/kb) and overall reaction times</li> <li><strong>Successful amplification of long products with higher yields</strong> &mdash; fragments up to 37 kb are amplified in less time and with less enzyme (0.25&ndash;1 unit/reaction)</li> </ul> <p>iProof Polymerase is available in an easy-to-use master mix and a PCR kit complete with controls.</p> <table class="pd_table pd_gridlines" style="text-align: left;" border="0"> <tbody> <tr> <td valign="top"><strong>iProof High-Fidelity DNA Polymerase</strong></td> <td align="center" valign="top"><strong>Number of Reactions</strong></td> <td align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity PCR Kit</td> <td align="center">50 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725330-iproof-high-fidelity-pcr-kit-50-u-2-u-ul-25-ul">1725330</a></td> </tr> <tr> <td>iProof High-Fidelity PCR Kit</td> <td align="center">200 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725331-iproof-high-fidelity-pcr-kit-200-u-2-u-ul-100-ul?ID=1725331">1725331</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof HF Master Mix</td> <td align="center">100 x 50 &micro;l reactions</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725310-iproof-hf-master-mix-100-x-50-ul-rxns-2-5-ml?ID=1725310">1725310</a></td> </tr> <tr> <td>iProof GC Master Mix</td> <td align="center">100 x 50 &micro;l reactions</td> <td align="center"><span style="font-size: 11px;">1725320</span></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">20 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725300-iproof-high-fidelity-dna-polymerase-20-u-2-u-ul-10-ul">1725300</a></td> </tr> <tr> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">100 U (2 U/&micro;l)</td> <td align="center"><a style="font-size: 11px;" href="/en-us/sku/1725301-iproof-high-fidelity-dna-polymerase-100-u-2-u-ul-50-ul">1725301</a></td> </tr> <tr class="pd_colorbackground"> <td>iProof High-Fidelity DNA Polymerase</td> <td align="center">500 U (2 U/&micro;l)</td> <td align="center"><a href="/en-us/sku/1725302-iproof-high-fidelity-dna-polymerase-500-u-2-u-ul-250-ul"><span style="font-size: 11px;">1725302</span></a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Categories</h2> <ul> <ul> <li><a href="/en-us/category/high-fidelity-standard-pcr-reagents">Standard PCR Enzymes and Reagents</a></li> <li><a href="/en-us/category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005">RNA Isolation Kits</a></li> <li><a href="/en-us/category/cdna-synthesis-kits?ID=M87EPGE8Z">Reverse Transcription Kits</a></li> <li><a href="/en-us/category/real-time-pcr-supermixes-kits?ID=M87F1J8UU">Real-time PCR Supermixes </a></li> </ul> </ul> <div id="wetLab"> <script type="text/javascript">// <![CDATA[ $('#wetLab').load('/webroot/web/html/lsr/products/amplification_pcr/wet-lab.html'); // ]]></script> </div> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>iProof High-Fidelity PCR Kit <a href="/webroot/web/pdf/lsr/literature/10002300B.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>iProof High-Fidelity Master Mix <a href="/webroot/web/pdf/lsr/literature/10002299.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual </a></li> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer">Brochure</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global iproof-premixed-pcr-reagents-1725320-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/iproof-premixed-pcr-reagents-1725320-desc.html 1708352 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-8352_0921120432022.xml 1708890 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Real-Time-PCR-Detection-System/170-8890_0921120538440.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725320&CartQuote=QT&quantity= false true false $304.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725320&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725320&quantity= 1725320
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Number Description Options
10002300 Instruction Manual, iProof High-Fidelity PCR Kit, Rev B Click to download
10002298 Instruction Manual, iProof High-Fidelity DNA Polymerase, Rev B Click to download
5211 iProof High-Fidelity DNA Polymerase Flier, Rev C Click to download [ Add to Cart (Free) ]
6252 Reagent Comparison Guide for Real-Time PCR Brochure, Rev C Click to download [ Add to Cart (Free) ]
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
5337 Long Inverse PCR Using iProof Polymerase, Rev A Click to download
10000083948 Instruction Manual, iProof High-Fidelity PCR Master Mix Click to download

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