ChemiDoc™ Imaging System

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ChemiDoc Imaging System

Overview

V3 Western Workflow™
Imaging System Selection Guide

  ChemiDoc MP ChemiDoc Touch Gel Doc XR+ GelDoc EZ GS-900
ChemiDoc™
MP
ChemiDoc™
ChemiDoc™
XRS+
Gel Doc™
XR+
Gel Doc™
EZ
GS-900™
Nucleic Acid Detection
Ethidium Bromide
SYBR® Green I and SYBR® Safe Dyes
Fast Blast™ DNA Stain
Protein Staining, 1-D Gels
Stain-Free Gels
Coomassie Blue Stain
Silver Stain
SYPRO Ruby, Flamingo, and Oriole Gel Stains
Protein Staining, 2-D Gels
Coomassie Blue Stain
Silver Stain
SYPRO Ruby, Flamingo, and Oriole Gel Stains
Cy2, Cy3, Cy5 Label
Blot Detection
Multiplex fluorescence
Immun-Star™
Chemiluminescence
Stain-free blots
Colorimetric blots
SYPRO Ruby Protein Blot Stain*

*  Optimal with low fluorescence PVDF membrane.


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ChemiDoc Touch
 
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The ChemiDoc Imaging System provides fast, reliable, and sensitive imaging and documentation of gels and chemiluminescence western blots. This system is compatible with stain-free technology, chemiluminescence detection, and a wide range of gel stains such as ethidium bromide, SYPRO Ruby, Coomassie, and silver stains.

The ChemiDoc Imaging System can be upgraded to gain the full fluorescent western blotting capabilities of the ChemiDoc MP System. The upgrade package has an increased spectrum (RGB, far red, and near infrared), allowing multiplex fluorescence western blotting capability with minimal cross-talk between channels. Multiplex fluorescence allows detection of multiple antigens on the same blot, eliminating the need for stripping and reprobing.

The ChemiDoc™ V3 Western Workflow™ provides efficient, consistent electrophoresis and blotting with the ability to rapidly assess electrophoresis and blot transfer quality, and perform total protein normalization.

Features and Benefits

  • All-in-one flexible imaging — get precise, reproducible chemiluminescence and colorimetric blot detection, analysis, and gel documentation in a single system
  • Sample Trays and Smart Tray Technology™ — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
  • Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminate the need for housekeeping proteins
  • Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help

Applications and Uses

  • Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
  • Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with chemiluminescence, or colorimetric detection

Included Components

  • Internal computer with Image Lab™ Touch Software
  • 12” touch screen display
  • Image Lab Software to install on your computer
  • Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)
  • Clarity™ (1705060) and Clarity Max™ (1705062) Western ECL Substrates; Precision Plus Protein™ All Blue Prestained (1610373) and Precision Plus Protein Unstained, Strep-tagged (1610363) Protein Standards

Mini and Midi ChemiDoc V3 Western Workflows include:

  • ChemiDoc Imaging System
  • Mini-PROTEAN® Tetra Cell, 4-gel (1658004) or Criterion™ Cell (1656001) Electrophoresis Chamber
  • PowerPac™ Basic Power Supply (1645050)
  • Trans-Blot® Turbo™ Transfer System (1704150)
  • 4–20% Mini-PROTEAN® TGX Stain-Free™ or 4–20% Criterion™ TGX Stain-Free™ Protein Gels
  • Buffers, Nitrocellulose Transfer Packs, Immun-Blot® LF PVDF Membranes, and other consumables

Optional Components

  • White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR® Stain (12003027)
  • Holder for ChemiDoc Sample Trays and UV Shield (1708377)
  • Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
  • UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)

Configuration Options

Product Catalog Number
ChemiDoc Imaging System 17001401
ChemiDoc V3 Western Workflow for Mini Gels 17001403
ChemiDoc V3 Western Workflow for Midi Gels 17001390
ChemiDoc to ChemiDoc MP Upgrade Kit 12003263

Note: Also available for increased capabilities are the ChemiDoc MP Imaging System (17001402), ChemiDoc MP V3 Western Workflows for Mini Gels (17001395), and ChemiDoc MP V3 Western Workflows for Midi Gels (17001389), which provide a broader spectrum (RGB, far red, and near infrared), multiplex fluorescence western blotting, and reduced crosstalk.

Related Products

Related Categories

Automation Capabilities  
Smart Tray Technology™ Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Autofocus Precalibrated focus for any zoom setting or sample height
Auto-exposure Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding Dynamic; precalibrated and optimized for each application
Hardware  
Touch-screen functionality Multitouch capability (2 points) 12.1" display
Maximum image area (W x H) 21 x 16.8 cm
Detector Cooled CCD, 6 megapixels
Dynamic range >4 orders of magnitude
Illumination sources Trans-UV, 302 nm excitation
Epi-white
Trans-white (requires White Sample Tray)
Trans-blue, 450–490 nm excitation (requires Blue Sample Tray)
Detection Chemiluminescence filter
590/110 nm standard emission filter for protein and DNA gel and blot imaging
Data output 16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight 35 kg (78 lb)
Instrument size (L x W x H) 61 x 51 x 53 cm
Operating voltage 100–250 V
Operating temperature 10–28°C
Operating humidity 10–85% relative humidity (non-condensing)
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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

ChemiDoc™ Imaging System

17001401
Gel and blot imaging and analysis system, includes internal computer, 12" touch-screen display, camera, Image Lab™ Touch Software, Image Lab Software , Blot/UV/Stain-Free Sample Tray, Clarity™ and Clarity Max™ Substrates, Precision Plus Protein™ All Blue Standards, Precision Plus Protein Unstained Standards

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ChemiDoc™ V3 Western Workflow™ for Mini Gels

17001403
Pkg of 1, complete suite of instruments, accessories, and consumables to run chemiluminescent western blotting experiments from start to finish. Uses precast mini gels. Includes Image Lab™ Software.

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ChemiDoc™ V3 Western Workflow™ for Midi Gels

17001390
Pkg of 1, complete suite of instruments and consumables to run chemiluminescent western blotting experiments from start to finish. Includes precast midi gels. Uses Image Lab™ Software.

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ChemiDoc™ to ChemiDoc MP Upgrade Kit

12003263
Pkg of 1, includes blue, green, red, far red, near infrared illumination sources and emission filters

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Number Description Options
6967 ChemiDoc™ Imaging System Flier Click to download
10000062126 ChemiDoc™ and ChemiDoc MP Imaging Systems with Image Lab™ Touch Software User Guide Click to download
6427 Bio-Rad® V3 Western Workflow™, Ver C Click to download [ Add to Cart (Free) ]
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide, Ver C Click to download [ Add to Cart (Free) ]