Bio-Plex Pro™ Human Acute Phase Multiplex Assays

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Overview

Available Bio-Plex Pro Disease Research Assays
Precision Pro™ Human Cytokines Human Isotyping
Human Diabetes
Human Acute Phase Human Angiogenesis
IL-1β
IgG1
Adiponectin
α-2-macroglobulin
Angiopoeitin-2
IL-2
IgG2
Adipsin
CRP
Follistatin
IL-4
IgG3
C-peptide
Ferritin
G-CSF
IL-5
IgG4
Ghrelin
Fibrinogen
HGF
IL-6
IgM
GIP
Haptoglobin
IL-8
IL-10
IgA
GLP-1
Procalcitonin
Leptin
IL-12 (p70)
IgE
Glucagon
SAA
PDGF-BB
IL-1β
 
IL-6
SAP
PECAM-1
IFN-γ
 
Insulin
Tissue plasminogen activator
VEGF
TNF-α
 
Leptin
 
   
PAI-1
   
   
Resistin
   
   
TNF-α
   
   
Visfatin
   
What You Need For Bio-Plex Pro Disease Research Assays
What is Needed for Performing Bio-Plex Pro Assay

Bio-Plex Pro assay panel — 1 x 96-well and 10 x 96-well, includes coupled magnetic beads, detection antibodies, standards, and controls.

Bio-Plex Pro reagent kit — includes assay buffer, wash buffer, streptavidin-PE, 96-well filter plate, sealing tape, and panel-specific instructions required to prepare assays and aquire data.

Bio-Plex diluent kit — choose the appropriate diluent kit for the panel for diluting samples, standards, and controls.

Bio-Plex® Technology and Applications

Disease research involving the study of inflammation, sepsis, cardiovascular disease, and diabetes often requires the measurement of multiple acute phase biomarkers. Bio-Plex Pro™ human acute phase immunoassays deliver accurate and reproducible measurements of nine of the most commonly measured biomarkers, using the Bio-Plex® 200 system. The multiplex immunoassays have optimal performance in serum, plasma, and tissue culture supernatant. Bio-Plex offers a 4-plex panel and a 5-plex panel, as well as a complete kit which contains both panels.

The 5-plex panel detects:

  • Ferritin
  • Fibrinogen
  • Procalcitonin
  • Serum amyloid A
  • Tissue plasminogen activator

The 4-plex panel detects:

  • α-2-macroglobin
  • C-reactive protein (CRP)
  • Haptoglobin
  • Serum amyloid P

Dividing the targets into two kits of distinct sample dilution requirements reduces the need to expend assay reagents on multiple dilutions. These assays are based on 8.0 µm magnetic beads making them compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Allows detection of 9 human acute phase biomarkers in 2 assays
  • Provides excellent sensitivity with both the automated washing system and a standard vacuum manifold
  • Eliminates manual filtration steps (with automated washing), increasing throughput and reproducibility
  • Includes controls for generating QC samples

Additional Neccessary Reagents

If a complete kit is purchased, no additional reagents are required. If the panels are purchased alone, the following additional items are needed to perform a Bio-Plex Pro human acute phase assay:

  • Bio-Plex Pro human acute phase reagent kit — includes assay reagents and instructions required to prepare samples and acquire data
  • Bio-Plex Pro human acute phase diluent kit — includes diluents for diluting samples, standards, and controls (one diluent kit is sufficient to perform both the 5-plex and the 4-plex assays)

Instrument Compatibility

This product is not compatible with the Bio-Plex® MAPGIX™ and Bio-Plex 3D systems due to the bead size.

More Information

See how Bio-Plex instruments and assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Species Human
Immunoassay for Acute phase
Format Premixed panel
Custom assay options Not available
Compatible with Bio-Plex® 200 system
Bead type Magnetic
Bio-Plex Pro Human Acute Phase 5- + 4-Plex Panel Complete Kit #171-A4S07M
Bio-Plex Pro™ Human Acute Phase 5- + 4-Plex Panel Complete Kit

171-A4S07M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls, reagents and diluents for detecting 9 human acute phase biomarkers

List Price:   $2,065.00
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Bio-Plex Pro Human Acute Phase 4-Plex Panel Complete Kit #171-A4C09M
Bio-Plex Pro™ Human Acute Phase 4-Plex Panel Complete Kit

171-A4C09M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls, reagents and diluents for detecting 4 human acute phase biomarkers

List Price:   $1,086.00
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Bio-Plex Pro Human Acute Phase 5-Plex Panel Complete Kit #171-A4C07M
Bio-Plex Pro™ Human Acute Phase 5-Plex Panel Complete Kit

171-A4C07M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls, reagents and diluents for detecting 5 human acute phase biomarkers

List Price:   $1,317.00
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Bio-Plex Pro Human Acute Phase 5-Plex Panel #171-A4007M
Bio-Plex Pro™ Human Acute Phase 5-Plex Panel

171-A4007M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls for detecting 5 human acute phase biomarkers, requires acute phase reagent kit, diluent kit

List Price:   $922.00
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Bio-Plex Pro Human Acute Phase 5-Plex Panel #171-A4008M
Bio-Plex Pro™ Human Acute Phase 5-Plex Panel

171-A4008M
10 x 96-well, includes coupled beads, detection antibodies, standards, and controls for detecting 5 human acute phase biomarkers, requires acute phase reagent kit and 10 diluent kits

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Bio-Plex Pro Human Acute Phase 4-Plex Panel #171-A4009M
Bio-Plex Pro™ Human Acute Phase 4-Plex Panel

171-A4009M
1 x 96-well, includes coupled beads, detection antibodies, standards, controls for detecting 4 human acute phase biomarkers, requires acute phase reagent kit and diluent kit

List Price:   $799.00
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Bio-Plex Pro Human Acute Phase 4-Plex Panel #171-A4010M
Bio-Plex Pro™ Human Acute Phase 4-Plex Panel

171-A4010M
10 x 96-well, includes coupled beads, detection antibodies, standards, and controls for detecting 4 human acute phase biomarkers, requires acute phase reagent kit and 10 diluent kits

List Price:   $7,380.00
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Number Description Options
5405 Bio-Plex Multiplex System Brochure, Rev D Click to download [ Add to Cart (Free) ]
10011597 Bio-Plex Pro Acute Phase Instruction Manual Rev A Click to download
5650 Bio-Plex Pro Human Acute Phase Assay Panel Product Sheet, Rev B Click to download [ Add to Cart (Free) ]
6335 Bio-Plex Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev C Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download