Overview
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Contact an ExpertThe Bio-Plex 200 system is a suspension array system which offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 100 biomolecules in a single sample.
Included System Components
- Array reader — combines 2 lasers, high-throughput fluidics, and real-time digital signal processing to distinguish up to 100 different color-coded bead sets, each representing a different assay
- Microplate platform — automates the reading of 96-well plates, yielding up to 9,600 data points in about 35 min
- Calibration and validation kits — system tools for daily calibration of signal output and for IQ/OQ and monthly validation of the primary performance parameters of the reader
- Bio-Plex MCV plate IV — maintenance, calibration, and validation (MCV) plate customized for hands-free startup, shutdown, and performance testing
- PC and monitor — includes Windows 10 operating system, Bio-Plex Manager, Microsoft Office and Adobe Acrobat Reader software, and a 17" flat-screen monitor"
- Bio-Plex Manager software — controls the instrument, data acquisition, and data analysis for superior curve fitting, statistics reporting, and charting features, without requiring the exporting or importing of files
- Bio-Plex SNP Manager™ data analysis utility — simple-to-use data analysis tool for nucleic acid applications
Bio-Plex Quick Tip Videos
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Bio-Plex Quick Tips — Planning for Assay Success Part 1
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Bio-Plex Quick Tips — Planning for Assay Success Part 2
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Bio-Plex Quick Tips — Planning for Assay Success Part 3
Related Technologies
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Specifications
Environment conditions
Operating temperature
15–30°C (59–86°F)
Operating humidity, noncondensing
20–80%
Operating altitude
Designed to operate at up to 2,000 m (6,600') mean sea level
Compensatory range
±2°C
UL installation category
UL Installation Category II, as defined in Annex J of UL 3101–1
Pollution degree
UL Pollution Degree 2, as defined in Section 3.7, 3.2, of UL 3101–1
Bio-Plex Array Reader Specifications
Input voltage range
100–240 V, 1.5 A, 47 – 63 Hz
Physical dimensions (W x D x H), cm
43 x 51 x 23
Weight, kg/lb
23/60
Lasers
Reporter laser
532 nm, >10 mW, frequency-doubled diode; 30 x 60 µm elliptical beam
Classification laser
635 nm, 10 mW, diode; 30 x 60 µm elliptical beam
Fluidics
Sheath flow rate, µl/sec
90
Cuvette
200 µm square flow channel
Sample injection rates, µl/sec
60
Electronics
Reporter channel detection
Photomultiplier tube, A/D resolution 14 bits
Classification and doublet discriminator channel detection
Avalanche photodiodes with temperature compensation, A/D resolution 12 bits
Communication interface
RS–232
Signal Processing
Measurement resolution
15 bits effective
Processor modes
Linear, with logarithmic or linear display option
Dynamic range, dB
70
Bio-Plex Microplate Platform Specifications
Input voltage range
100–240 V, 1.8 A, 47–63 Hz
Physical dimensions (W x D x H), cm
44 x 61 x 8
Weight, kg/lb
14.4 (32)
Communications interface
RS–232
Plate capacity
One 96-well microtiter plate, ≤0.75
Ordering
items
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(Discontinued) Bio-Plex® 200 System
171000201
100–240 V, multiplex array system, includes reader, microplate platform, Bio-Plex Manager™ 6.0 software, PC, calibration kit, validation kit 4.0, MCV plate IV, reservoir, sheath fluid, 2 sample needles
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(Discontinued) Bio-Plex® 200 System With HTF
171000205
100–240 V, multiplex array system (#171-000201) with high-throughput fluidics
Accessories
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(Discontinued) Bio-Plex® Handheld Magnetic Washer
171020100
Includes magnetic washer and adjustment hex tools for use in manual wash steps for all Bio-Plex Magnetic Assays
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(Discontinued) Sheath Fluid
171000055
20 L, 1x fluid in cube container with pour spout, for use with the Bio-Plex® 200 and Bio-Plex 3D system array readers and high-throughput fluidics (HTF) system
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(Discontinued) Sheath Fluid Bottle
171002010
Pkg of 1, 1 L polypropylene bottle with sheath-fluid and air-intake ports and tubing, for use with the Bio-Plex 200 system
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(Discontinued) Sheath Waste Bottle
171002012
Pkg of 1, 1 L polypropylene bottle with waste port and tubing, for use with the Bio-Plex 200 system
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(Discontinued) Sample Needle
171002020
Pkg of 1, 11.6 cm (4.6"), long stainless steel sampling needle for use with the Bio-Plex 200 system
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(Discontinued) Alignment Guide with Shipping Pin
171002024
Pkg of 1, replacement fitting and blue shipping pin for Bio-Plex 200 system; aligns array reader on microplate platform
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(Discontinued) Needle Adjustment Wrench
171002026
Pkg of 1, tool for adjusting sample needle on the Bio-Plex 100 system
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(Discontinued) Protective Shield for Sample Needle
171002030
Pkg of 1, covering for sample needle on the Bio-Plex 200 system
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(Discontinued) Air Intake Filter
171002032
Pkg of 1, replacement maintenance part for the Bio-Plex 200 system
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(Discontinued) Syringe Seal
171002033
Pkg of 4, replacement maintenance parts for syringe plunger on the Bio-Plex 200 system
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(Discontinued) Syringe Seal With Cylinder
171002034
Pkg of 1, replacement syringe assembly for internal fluidics system on the Bio-Plex 200 system
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(Discontinued) Sheath Fluid Filter With Quick Connect Tubing
171002038
Pkg of 1, replacement maintenance part for internal fluidics system on the Bio-Plex 200 system; removes >5 μm diameter particles
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(Discontinued) Sheath Cube Filter
171002040
Pkg of 1, replacement maintenance part for use with high-throughput fluidics (HTF) system and Bio-Plex 200 system; removes >10 µm diameter particles
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(Discontinued) HTF Tubing
171002056
Pkg of 1, air tubing HTF to reader, sheath tubing HTF to reader, tubing sheath container to HTF (including 10 µm filter), and waste tubing (reader to waste container)
Documents
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Number Description Options
6006
Bio-Plex MAGPIX, Bio-Plex 200, and Bio-Plex 3D Systems – Comparable Performance Across the Board, Product Information Sheet, Rev A
6457
The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B
6156
Composite Profiling of Angiogenic Factors Using Bio-Plex Pro Human Cancer Biomarker Panel 1, Rev A
3076
Bio-Plex Care and Maintenance Quick Guide, Rev B
2993
Bio-Plex Glossary, Rev A
4100205
Bio-Plex Maintenance Log, Rev A
5336
Bio-Plex Suspension Array System Nucleic Acid Analysis Flier, Rev B
3000
Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
10005042
Instruction Manual, Bio-Plex 200 System Hardware Instruction, Rev G
4100180
Instruction Manual, Bio-Plex Protein Array System High Throughput Fluidics (HTF) System, Rev A
5578
Xenograft Samples By Bio-Plex Tech note, Rev A
2879
Bio-Plex System Tools Brochure, Rev C
6458
Bio-Plex Success Guide/Assay Sample Prep, Rev B