pGLO kits utilize Bio-Rad’s pGLO plasmid, which encodes a green fluorescent protein (GFP), to enable instructors to give students a hands-on introduction to transformation, cloning, protein chromatography, and electrophoresis techniques.
In this activity, students transform bacteria by introducing a gene from the bioluminescent jellyfish Aequorea victoria. The pGLO kit follows the same procedure used by molecular biologists to create "designer proteins."
Use this GFP kit to take your students through the process of creating a new product — from discovery in the laboratory to biomanufacturing to market.
This GFP kit is a fantastic way to introduce your students to the world of commercial biotechnology by involving them in a simulated scenario as employees at a company in the drug discovery business.
This activity extends the utility of the pGLO kit by using the green fluorescent protein expressed in bacteria to illustrate the central dogma of biology: DNA transcription, the expression of a protein, and the visualization of a trait.
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