pGLO™ SDS-PAGE Extension

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pGLO SDS-PAGE Extension

Overview

Lab Preparation Checklist

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)

Dithiothreitol (DTT), 1 g
1
Precision Plus Protein Kaleidoscope standards, 50 µl  2
Bio-Safe Coomassie stain for proteins, 100 ml
1
10x Tris-glycine-SDS electrophoresis buffer, 1 L
1
Laemmli sample buffer, 30 ml
1
Curriculum, including Teacher's Guide and Student Manual
1

Required Accessories Not Included in Kit:

Amp (white) and amp/ara (green) colonies from plates from the pGLO bacterial transformation lab 4–8
Mini-PROTEAN® TGX precast polyacrylamide gels, 4–20%, 10-well each 4–8
Vertical gel electrophoresis chambers 1–4
Power supplies 1–4
Water bath or dry bath 1
Adjustable micropipets
   2–20 µl
   100–1,000 µl

4–8
4–8
Pipet tips
   2–200 µl, BR-35
   100–1,000 µl, BR-40
   0.5–200 ul, Prot/Elec

1 bag
1 bag
1 bag
Screwcap microcentrifuge tubes, 1.5 ml 500

Recommended (Optional) Accessories:

GelAir drying system
GelAir assembly table
GelAir drying frames with clamps
GelAir cellophane
Molecular Imager® GelDoc XR system
pGLO Kit SDS-PAGE Extension Flowchart
pGLO SDS-PAGE Extension Flowchart
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The pGLO™ Kit SDS-PAGE Extension is designed to be performed following the pGLO Bacterial Transformation Kit. The extension is designed to identify and visualize green fluorescent protein (GFP) from the proteome of transformed E. coli, which also contains thousands of other proteins. Because only the cloned GFP glows, students can use this phenotypic trait to quickly identify the GFP within the complex mix of proteins.

Features and Benefits

With this kit, students are able to:

  • Perform real biotechnology workflows
  • Identify proteins by conformation and size
  • Link gene induction to protein expression to protein identification
  • Understand chromophores and the basis of protein fluorescence
  • Extend the pGLO Bacterial Transformation Kit

How It Works

In the first part of the exercise, students use the pGLO Bacterial Transformation Kit to transfer pGLO plasmid encoding GFP into E. coli, a common prokaryotic organism used for DNA propagation and protein expression. Colonies of E. coli are qualitatively examined for fluorescence to determine whether the pGLO gene is being expressed. In the second part of the laboratory activity, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is employed to separate the entire repertoire of proteins expressed in E. coli, including the foreign GFP responsible for transferring the fluorescence trait.

More Information

  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation.
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Laemmli sample buffer, 30 ml 1
Precision Plus Protein™ Kaleidoscope™ prestained standards, 50 µl 2
Dithiothreitol (DTT), 1 g 1
10x Tris-glycine-SDS electrophoresis buffer, 1 L 1
Bio-Safe™ Coomassie stain for proteins, 100 ml 1
Curriculum, including Teacher's Guide and Student Manual 1
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pGLO Kit SDS-PAGE Extension

1660013EDU
Protein analysis classroom study kit, includes DTT, protein standards, Bio-Safe Coomassie stain, 10x TGS, Laemmli sample buffer, curriculum, for 32 students; education use only

2 1660013EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/pGLO-SDS-PAGE-Extension/166-0013EDU_0921121041298.xml 1660013EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:24 1660013edu-pglo-kit-sds-page-extension 1660013EDU pGLO Kit SDS-PAGE Extension pGLO Kit SDS-PAGE Extension Protein analysis classroom study kit, includes DTT, protein standards, Bio-Safe Coomassie stain, 10x TGS, Laemmli sample buffer, curriculum, for 32 students; education use only Protein analysis classroom study kit, includes DTT, protein standards, Bio-Safe Coomassie stain, 10x TGS, Laemmli sample buffer, curriculum, for 32 students; education use only Protein analysis classroom study kit, includes DTT, protein standards, Bio-Safe Coomassie stain, 10x TGS, Laemmli sample buffer, curriculum, for 32 students; education use only /webroot/web/images/lse/products/pglo_gfp_kits/product_thumb/global/lse_pglo_kit_sdspage_ex_pvtn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/product_detail/global/lse_pglo_kit_sdspage_ex.jpg RepEx 166-0013EDU Protein analysis classroom study kit, includes DTT, protein standards, Bio-Safe Coomassie stain, 10x TGS, Laemmli sample buffer, curriculum, for 32 students; education use only 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/pGLO-SDS-PAGE-Extension N 0 166-0013EDU, 1660013EDU, pglo, sds-page, extension, dna, transcription, protein, gene, gfp, expression, visualization, sds, page, gel, electrophoresis, science, education, product, classroom, lab, prep, map, pglow, sequence, plasmide false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660013EDU&CartQuote=QT&quantity= false true false $128.00 true %2496.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660013EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660013EDU&quantity= 1660013EDU
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pGLO Kit SDS-PAGE Extension Bagged Instruction Manual

1660023EDU
Instruction manual for use with pGLO Kit SDS-PAGE Extension (#166-0013); education use only

1660023EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/pGLO-SDS-PAGE-Extension/166-0023EDU_0921121041359.xml 0 1660023EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/pGLO-SDS-PAGE-Extension/166-0023EDU_0921121041359.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:24 1660023edu-pglo-kit-sds-page-extension-bagged-instruction-manual 1660023EDU pGLO Kit SDS-PAGE Extension Bagged Instruction Manual pGLO Kit SDS-PAGE Extension Bagged Instruction Manual Instruction manual for use with pGLO Kit SDS-PAGE Extension (#166-0013); education use only Instruction manual for use with pGLO Kit SDS-PAGE Extension (#166-0013); education use only Instruction manual for use with pGLO Kit SDS-PAGE Extension (#166-0013); education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0023EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0023EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0023EDU_feature.jpg RepEx 166-0023EDU Instruction manual for use with pGLO Kit SDS-PAGE Extension (#166-0013); education use only 166-0023EDU, 1660023EDU, pglo, sds-page, extension, bagged, instruction, manual, dna, transcription, protein, gene, gfp, expression, visualization, sds, page, gel, electrophoresis, science, education, product, classroom, 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/pGLO-SDS-PAGE-Extension N 0 <p>pGLO™ Kit SDS-PAGE Extension Bagged Instruction Manual includes curriculum, teacher's guide, student manual, and graphic quick guide. The Instruction Manual is for use with pGLO Kit SDS-PAGE Extension (#166-0013). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 166-0023EDU_0921121041359_en.html /webroot/web/html/lse/products/SKUs/autopopulated/166-0023EDU_0921121041359_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660023EDU&CartQuote=QT&quantity= false true false $18.75 true %2415.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660023EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660023EDU&quantity= 1660023EDU
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Dithiothreitol

1610610EDU
1 g, dithiothreitol (DTT) reducing agent; education use only

1610610EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0610EDU_0921121024180.xml 1 1610610EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:21 1610610edu-dithiothreitol 1610610EDU Dithiothreitol Dithiothreitol 1 g, dithiothreitol (DTT) reducing agent; education use only 1 g, dithiothreitol (DTT) reducing agent; education use only 1 g, dithiothreitol (DTT) reducing agent; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/161-0610EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/161-0610EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/161-0610EDU_feature.jpg RepEx 161-0610EDU 1 g, dithiothreitol (DTT) reducing agent; education use only 1610610, 1610610EDU, dithiothreitol, dtt, reducing, agent, cleland's, reagent, redox, antioxidant, stabilizer, electrophoresis, buffer, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>Dithiothreitol (DTT), 1 g, is a reducing agent used in SDS-PAGE sample loading buffer. Dithiothreitol is a refill component for the pGLO™ Kit SDS-PAGE Extension (#166-0013EDU) and for the Comparative Proteomics Kit I: Protein Profiler Module (#166-2700EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 161-0610EDU_0921121024180_en.html /webroot/web/html/lse/products/SKUs/autopopulated/161-0610EDU_0921121024180_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610610EDU&CartQuote=QT&quantity= false true false $28.00 true %2422.40 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610610EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610610EDU&quantity= 1610610EDU
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Precision Plus Protein Kaleidoscope Standards

1610375EDU
500 µl, mixture of 10 multicolor recombinant proteins (10–250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only

1610375EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0375EDU_0921121023611.xml 3 1610375EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0375EDU_0921121023611.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:09:36 1610375edu-precision-plus-protein-kaleidoscope-standards 1610375EDU Precision Plus Protein Kaleidoscope Standards Precision Plus Protein<sup>&trade;</sup> Kaleidoscope<sup>&trade;</sup> Standards 500 &#181;l, mixture of 10 multicolor recombinant proteins (10&ndash;250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only 500 &#181;l, mixture of 10 multicolor recombinant proteins (10&ndash;250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only 500 &#181;l, mixture of 10 multicolor recombinant proteins (10&ndash;250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0375_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0375_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0375_feature.jpg RepEx 161-0375EDU 500 &#181;l, mixture of 10 multicolor recombinant proteins (10&ndash;250 kD), including three bands (25, 50, 75 kD) that match the reference bands of other standards, 50 applications; education use only 161-0375EDU, 1610375EDU, precision plus protein, kaleidoscope, standards, multicolor, standard, markers, marker, molecular, weight, mw, ladders, ladder, prestained, science, education, productspeptide, polypeptide 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>Precision Plus Protein&trade; Kaleidoscope&trade; Standards (500 &micro;l, 50 applications) are a mixture of 10 multicolor recombinant proteins (10&ndash;250 kD) and include three bands (25, 50, 75 kD) that match the reference bands of other standards.</p> <p>Precision Plus Protein Kaleidoscope Standards can be used as a refill for the <a href="/en-us/sku/1660013edu-pglo-kit-sds-page-extension?ID=1660013EDU">pGLO&trade; Kit</a> SDS-PAGE Extension (<a href="/en-us/sku/1660013edu-pglo-kit-sds-page-extension?ID=1660013EDU">#1660013EDU</a>), <a href="/en-us/sku/1662700edu-comparative-proteomics-kit-i-protein-profiler-module?ID=1662700EDU">Comparative Proteomics Kit I:</a> Protein Profiler Module (<a href="/en-us/sku/1662700edu-comparative-proteomics-kit-i-protein-profiler-module?ID=1662700EDU">#1662700EDU</a>), and the <a href="/en-us/sku/1662850edu-comparative-proteomics-kits-i-ii-protein-profiler-module-western-blot-module?ID=1662850EDU">Comparative Proteomics Kits I and II:</a> Protein Profiler Module and Western Blot Module (<a href="/en-us/sku/1662850edu-comparative-proteomics-kits-i-ii-protein-profiler-module-western-blot-module?ID=1662850EDU">#1662850EDU</a>). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 161-0375EDU_0921121023611_en.html /webroot/web/html/lse/products/SKUs/autopopulated/161-0375EDU_0921121023611_en.html 4561083EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Electrophoresis-and-Blotting-A/456-1083EDU_1343345186.xml 1610732EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0732EDU_0921121020153.xml 1610737EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0737EDU_0921121020190.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610375EDU&CartQuote=QT&quantity= false true false $144.00 true %24100.80 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610375EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610375EDU&quantity= 1610375EDU
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Bio-Safe™ Coomassie Stain

1610786EDU
1 L, premixed protein staining solution for polyacrylamide gels; education use only

1610786EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0786EDU_0921121024990.xml 4 1610786EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:21 1610786edu-bio-safe-coomassie-stain 1610786EDU Bio-Safe Coomassie Stain Bio-Safe&trade; Coomassie Stain 1 L, premixed protein staining solution for polyacrylamide gels; education use only 1 L, premixed protein staining solution, for polyacrylamide gels; education use only 1 L, premixed protein staining solution for polyacrylamide gels; education use only /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0786_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0786_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0786_feature.jpg RepEx 161-0786EDU 1 L, premixed protein staining solution, for polyacrylamide gels; education use only Bio-Safe Coomassie Stain, SDS-PAGE Extension, classroom refill kit 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>Bio-Safe Coomassie Stain is a premixed protein staining solution for polyacrylamide gels. This stain is a refill consumable for the <a href="/en-us/product/pglo-sds-page-extension">pGLO&trade; Kit SDS-PAGE Extension</a>. This product is for education use only.</p> <ul> <li>No requirement for fixation* with acetic acid and methanol</li> <li>Destain with water</li> <li>No handling and disposal of solvents</li> <li>Gels can be stored in water</li> </ul> <br /><br /> <p class="caption" style="margin-top:-20px">* Fixation is recommended for peptide and very low molecular weight protein separations prior to using Bio-Safe Coomassie Stain.</p> <h2 class="descHeader">Packaging Options</h2> <p>Bio-Safe Coomassie Stain is also available as a 5 L cube (<a href="/en-us/sku/1610787edu-bio-safe-coomassie-stain">1610787EDU</a>)</p> <h2 class="descHeader">Related Products</h2> <ul> <li> <a href="/en-us/product/pglo-bacterial-transformation-kit?">pGLO Bacterial Transformation Kit</a></li> <li><a href="/en-us/product/green-fluorescent-protein-chromatography-kit"></a><a href="/en-us/product/green-fluorescent-protein-chromatography-kit">Green Fluorescent Protein Chromatography Kit</a> </li> <li><a href="/en-us/product/secrets-rainforest-kit">Secrets of the Rainforest&trade; Kit</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li> <a href="/en-us/product/protein-expression-purification-series">Protein Expression and Purification Series</a></li> <li><a href="/en-us/product/comparative-proteomics-kit-i-protein-profiler-module">Comparative Proteomics Kit I: Protein Profiler Module</a></li> <li><a href="/en-us/product/polyacrylamide-reagents-precast-gels">Polyacrylamide Reagents and Precast Gels</a></li> <li><a href="/en-us/product/protein-electrophoresis-buffers-reagents">Protein Electrophoresis Buffers and Reagents</a></li> <li><a href="/en-us/product/protein-electrophoresis-size-standards">Protein Electrophoresis Size Standards</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li> <a href="/webroot/web/pdf/lse/literature/Bulletin_6672.pdf " target="_blank">Map of AP-Aligned Bio-Rad Kits with Essential Knowledge</a></li> <li><a href="/webroot/web/pdf/lse/literature/Bulletin_6673.pdf" target="_blank">Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories</a></li> <li>Refresh Kit Components &mdash; Large Class/Multiple Class <a href="/webroot/web/pdf/lse/literature/Bulletin_5891A.pdf" target="_blank">Preparation Guide</a></li> <li>Biotechnology Explorer&trade; Refresh Kit Components <a href="/webroot/web/pdf/lse/literature/Bulletin_5872.pdf" target="_blank">Purchasing Guide</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li> <a href="/en-us/applications-technologies/classroom-resources">Classroom Resources</a> </li> <li><a href="/en-us/applications-technologies/presentations-activities-for-workshops-teaching">Presentations and Activities for Workshops and Teaching</a> </li> </ul> /webroot/web/html/lse/products/SKUs/autopopulated 1-L- bio-safe-coomassie-stain-161-0786EDU-desc.html /webroot/web/html/lse/products/SKUs/autopopulated/1-L- bio-safe-coomassie-stain-161-0786EDU-desc.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610786EDU&CartQuote=QT&quantity= false true false $84.00 true %2467.20 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610786EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610786EDU&quantity= 1610786EDU
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10x Tris/Glycine/SDS Electrophoresis Buffer

1610732EDU
1 L, 10x premixed protein electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3; education use only

1610732EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0732EDU_0921121020153.xml 5 1610732EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:21 1610732edu-10x-tris-glycine-sds-electrophoresis-buffer 1610732EDU 10x Tris/Glycine/SDS Electrophoresis Buffer 10x Tris/Glycine/SDS Electrophoresis Buffer 1 L, 10x premixed protein electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3; education use only 1 L, 10x premixed protein electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3; education use only 1 L, 10x premixed protein electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3; education use only /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0732_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0732_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0732_feature.jpg RepEx 161-0732EDU 1 L, 10x premixed protein electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3; education use only 161-0732EDU, 1610732EDU, 10x, tris/glycine/sds, electrophoresis, buffer, tris, glycine, sds, gel, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>10x Tris/Glycine/SDS Electrophoresis Buffer (1 L) is a 10x premixed protein electrophoresis buffer for SDS-PAGE electrophoresis. The buffer contains 25 mM Tris, 192 mM glycine, and 0.1% SDS, pH 8.3. </p> <p>10x Tris/glycine/SDS Electrophoresis Buffer is a refill consumable for the pGLO™ Kit SDS-PAGE Extension (#166-0013EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 161-0732EDU_0921121020153_en.html /webroot/web/html/lse/products/SKUs/autopopulated/161-0732EDU_0921121020153_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610732EDU&CartQuote=QT&quantity= false true false $29.00 true %2423.20 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610732EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610732EDU&quantity= 1610732EDU
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2x Laemmli Sample Buffer

1610737EDU
30 ml, premixed protein sample buffer for SDS-PAGE; education use only

1610737EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/161-0737EDU_0921121020190.xml 6 1610737EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2018-02-01 00:09:36 1610737edu-2x-laemmli-sample-buffer 1610737EDU Laemmli Sample Buffer 2x Laemmli Sample Buffer 30 ml, premixed protein sample buffer for SDS-PAGE; education use only 30 ml, premixed protein sample buffer for SDS-PAGE; education use only 30 ml, premixed protein sample buffer for SDS-PAGE; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/161-0737EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/161-0737EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/161-0737EDU_feature.jpg RepEx 161-0737EDU 30 ml, premixed protein sample buffer for SDS-PAGE; education use only 161-0737EDU, 1610737EDU, 2x, laemmli, sample, buffer, premixed, sds, sds-page, page, polyacrylamide, gel, electrophoresis, science, education, products 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>Dilute protein samples 1:1 in 2x Laemmli sample buffer before loading on SDS-PAGE gels (requires the addition of &beta;-mercaptoethanol).</p> <p><strong>Other Information:</strong><br /></p> <ul> <li>Formulation: 65.8 mM Tris-HCl, pH 6.8, 2.1% SDS, 26.3% (w/v) glycerol, 0.01% bromophenol blue</li> <li>Ready to dilute &mdash; use distilled deionized water</li> <li>Premixed loading buffer &mdash; ensures lane-to-lane consistency</li> <li>Quality-controlled reagent &mdash; guarantees reproducible results</li> </ul> <p><strong>Related Products</strong></p> <p>We offer additional <a href="/evportal/destination/commerce/product_detail?catID=09e4dbf1-40c5-4a92-ace5-3ec31296ac7e">sample buffers and reagents</a> for protein electrophoresis including the following sample buffers:</p> <ul> <li><a href="/en-us/sku/1610747-4x-laemmli-sample-buffer?ID=1610747">4x Laemmli sample buffer (1610747)</a></li> <li><a href="/en-us/sku/1610738edu-native-page-sample-buffer?ID=1610738EDU">Native PAGE sample buffer (1610738EDU)</a></li> <li><a href="/en-us/sku/1610739-tricine-sample-buffer-for-protein-gels-30-ml?ID=1610739">Tricine sample buffer (1610739)</a></li> <li><a href="/en-us/sku/1610763-ief-sample-buffer-for-vertical-gels-30-ml?ID=1610763">IEF sample buffer (1610763)</a></li> <li><a href="/en-us/sku/1610764-zymogram-sample-buffer-30-ml?ID=1610764">Zymogram sample buffer (1610764)</a></li> <li><a href="/en-us/sku/1610791-xt-sample-buffer?ID=1610791">XT sample buffer (1610791)</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <p><a href="/webroot/web/pdf/lsr/literature/4006028.pdf" target="_blank">Download the Laemmli sample buffer instruction manual</a>.</p> <h2 class="descHeader">Instructions for Use:</h2> <p><strong>Step 1:</strong> Prepare 2x sample buffer containing &beta;-mercaptoethanol (BME) by mixing 950 &micro;l of 2x Laemmli sample buffer (161-0737) with 50 &micro;l of BME (1610710);</p> <p><strong>Step 2:</strong> Dilute protein sample 1:1 in sample buffer from Step 1. For example: 100 &micro;l protein sample + 100 &micro;l 2x sample buffer containing BME;</p> <p><strong>Step 3:</strong> Heat sample at 95&deg;C for 5 min. Load samples onto SDS-PAGE gels.</p> <ul> <br /> </ul> /webroot/web/html/lse/products/SKUs/autopopulated 161-0737EDU_0921121020190_en.html /webroot/web/html/lse/products/SKUs/autopopulated/161-0737EDU_0921121020190_en.html Laemmli sample buffer, 30 ml 2487 1 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610737EDU&CartQuote=QT&quantity= false true false $14.00 true %2411.20 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610737EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610737EDU&quantity= 1610737EDU
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6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories Click to download
M1660023 Instruction Manual, Protein Electrophoresis of GFP: A pGLO Bacterial Transformation Kit Extension, Biotechnology Explorer, Rev F Please login to download this document

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