Green Fluorescent Protein Chromatography Kit

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Green Fluorescent Protein Chromatography Kit

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Ampicillin, lyophilized 1
Arabinose, lyophilized 1
Lysozyme, lyophilized 1
LB nutrient broth tablet 1
Binding buffer 1
Column equilibration buffer 1
Column wash buffer 1
Elution buffer 1
HIC chromatography columns 8
Inoculation loops, sterile 20
Disposable plastic transfer pipets 40
Micro test tubes, 2.0 ml, clear 30
Cell culture tubes, 15 ml, sterile 25
Sample collection tubes, 5 ml 25
Foam micro test tube holders 8
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Transformed bacterial colonies from pGLO bacterial transformation kit  
UV lamps 4–8
Centrifuges 1–2
Recommended (Optional) Accessories:
Incubation oven  
Rocking platform  
Microwave oven  
GFP Chromatography Kit Workflow
GFP Chromatography Kit Workflow
Key Kit Features
  • Transform bacteria with a gene from a bioluminescent jellyfish (AP Lab 6)
  • Induce transformed bacteria to overexpress Green Fluorescent Protein
  • Purify GFP using chromatography
  • Complete in three 45 minute lab sessions
Curriculum Fit
Curriculum Fit
GFP Chromatography

Chromatography 101. Chromatography to purify proteins of interest depends on a protein's chemical or physical properties, such as molecular weight, electrical charge, or solubility. Green Fluorescent Protein (GFP) is extremely hydrophobic compared to bacterial proteins. This unique characteristic of GFP enables the purification of GFP from bacterial cell proteins using hydrophobic interaction chromatography (HIC). When placed in a buffer containing a high concentration of salt, the HIC matrix selectively binds hydrophobic GFP molecules while allowing the bacterial proteins to pass right through the column. Then, simply lowering the salt concentration of the buffer causes GFP to elute from the column in pure form.

pGLO Bacterial Transformation plate and a Series of HIC columns showing the separation of GFP.

pGLO Bacterial Transformation plate and a Series of HIC columns showing the separation of GFP.

PowerPoint Presentation for Workshops and for Teaching

pGLO Transformation and Green Fluorescent Protein Chromatography Kits

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the pGLO Transformation and Green Fluorescent Protein Chromatography Kits and includes instructions for using the kits.

pGLO Transformation and Green Fluorescent Protein Chromatography Kits Download PowerPoint presentation
pGLO Transformation and Green Fluorescent Protein Chromatography Kits
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The Green Fluorescent Protein Chromatography Kit can be used following the pGLO™ Bacterial Transformation Kit, which is purchased separately. A colony of transformed bacteria (produced using the pGLO Bacterial Transformation Kit) is placed in liquid cultures overnight, then lysed to release the bacteria's cellular contents. GFP is purified from the bacterial lysate using hydrophobic interaction chromatography (HIC) columns provided in this kit.

Features and Benefits

  • Aligns with AP Biology Lab 6
  • Fluorescent GFP allows real-time monitoring of the extraction and purification processes
  • Uses bacteria transformed with the pGLO Bacterial Transformation Kit

How It Works

Green fluorescent protein is extremely hydrophobic compared to bacterial proteins. Unique characteristics of GFP enable it to be purified from bacterial cell proteins using HIC columns. When placed in a buffer containing a high concentration of salt, the HIC matrix selectively binds hydrophobic GFP molecules while allowing the bacterial proteins to pass through the column. Then, simply lowering the salt concentration of the buffer causes GFP to elute from the column in pure form. pGLO bacterial transformation and GFP purification together explore key processes used in biotechnology to produce and purify "designer proteins" with commercial or research value.

More Information

  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Ampicillin, lyophilized 1
Arabinose, lyophilized 1
Lysozyme, lyophilized 1
LB nutrient broth tablet 1
Binding buffer 1
Column equilibration buffer 1
Column wash buffer 1
Elution buffer 1
HIC chromatography columns 8
Inoculation loops, sterile 20
Disposable plastic transfer pipets 40
Micro test tubes, 2.0 ml, clear 30
Cell culture tubes, 15 ml, sterile 25
Sample collection tubes, 5 ml 25
Foam micro test tube holders 8
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
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Green Fluorescent Protein Chromatography Kit

1660005EDU
Protein purification classroom kit, includes lyophilized reagents, nutrient broth tablet, buffers, HIC columns, pipets, test tubes, curriculum, and more, for 32 students; education use only

0 1660005EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Green-Fluorescent-Protein-Chro/166-0005EDU_0921121040929.xml 1660005EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbjo 2017-11-14 07:07:24 true true internet/product_SKU jthvlbjo templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Green-Fluorescent-Protein-Chro/166-0005EDU_0921121040929.xml LSE 2019-04-06 07:36:08 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2019-04-06 07:36:08 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Green-Fluorescent-Protein-Chro/166-0005EDU_0921121040929.xml templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Green-Fluorescent-Protein-Chro/166-0005EDU_0921121040929.xml 1660005edu-green-fluorescent-protein-chromatography-kit 1660005EDU Green Fluorescent Protein Chromatography Kit Green Fluorescent Protein Chromatography Kit Protein purification classroom kit, includes lyophilized reagents, nutrient broth tablet, buffers, HIC columns, pipets, test tubes, curriculum, and more, for 32 students; education use only Protein purification classroom kit, includes lyophilized reagents, nutrient broth tablet, buffers, HIC columns, pipets, test tubes, curriculum, and more, for 32 students; education use only Protein purification classroom kit, includes lyophilized reagents, nutrient broth tablet, buffers, HIC columns, pipets, test tubes, curriculum, and more, for 32 students; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0005EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0005EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0005EDU_feature.jpg preview RepEx 166-0005EDU Protein purification classroom kit, includes lyophilized reagents, nutrient broth tablet, buffers, HIC columns, pipets, test tubes, curriculum, and more, for 32 students; education use only 166-0005EDU, 1660005EDU, green, fluorescent, protein, chromatography, gfp, bacterial, expression, purification, science, education, products, protein biology 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/Green-Fluorescent-Protein-Chro N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660005EDU&CartQuote=QT&quantity= 1711 false true false $123.75 true %2499.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660005EDU&AddToDefaultWishList=true&quantity= true false true 12161500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660005EDU&quantity= 1660005EDU
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Lyophilized Components Refill Pack

1660015EDU
Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only

1660015EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0015EDU_0921121040371.xml 0 1660015EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlb9w 2017-11-14 07:07:24 true true internet/product_SKU jthvlb9w templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0015EDU_0921121040371.xml LSE 2019-04-06 07:35:41 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2019-04-06 07:35:41 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0015EDU_0921121040371.xml templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0015EDU_0921121040371.xml 1660015edu-lyophilized-components-refill-pack 1660015EDU Lyophilized Components Refill Pack Lyophilized Components Refill Pack Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0015EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0015EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0015EDU_feature.jpg Lyophilized Components Refill Pack #166-0015EDU RepEx Lyophilized Components Refill Pack #166-0015EDU Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only 1660015, 1660015EDU, lyophilized, components, refill, pack, gfp, chromatography, secrets, of, the, rainforest, ampicillin, arabinose, lb, nutrient, broth, tablet, lysozyme, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 <p>The Lyophilized Components Refill Package contains consumable reagents for the Green Fluorescent Protein Chromatography Kit (#166-0005EDU) and the Secrets of the Rainforest&trade; Kit (#166-0006EDU). The package includes ampicillin, arabinose LB nutrient broth tablet, and lysozyme. This product is for education use only. </p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0015EDU_0921121040371_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0015EDU_0921121040371_en.html 1158 <b>Refill contains sufficient lyophilized reagents for 8 student workstations (2&ndash;4 students per workstation)</b> &nbsp; 1 0 2453 Ampicillin, lyophilized 1 1 0 1065 Arabinose, lyophilized 1 1 0 1076 Lysozyme, lyophilized 1 1 0 1075 LB nutrient broth tablet 1 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660015EDU&CartQuote=QT&quantity= 1711 false true false $81.25 true %2465.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660015EDU&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660015EDU&quantity= 1660015EDU
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GFP Chromatography Buffers Refill Pack

1660016EDU
Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only

1660016EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml 1 1660016EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbac 2017-11-14 07:07:24 true true internet/product_SKU jthvlbac templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml LSE 2019-04-06 07:35:42 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2019-04-06 07:35:42 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml 1660016edu-gfp-chromatography-buffers-refill-pack 1660016EDU GFP Chromatography Buffers Refill Pack GFP Chromatography Buffers Refill Pack Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0016EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0016EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0016EDU_feature.jpg GFP Chromatography Buffers Refill Pack #166-0016EDU RepEx GFP Chromatography Buffers Refill Pack #166-0016EDU Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only 1660016, 1660016EDU, gfp, chromatography, buffers, refill, pack, consumables, replacement, buffer, secrets, of, the, rainforest, binding, equilibration, wash, elution, reagent, reagents, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 <p>Green Fluorescent Protein Chromatography Kit Lyophilized Components Refill Package contains consumable buffers for the Green Fluorescent Protein Chromatography Kit (#166-0005EDU) and the Secrets of the Rainforest&trade; Kit (#166-0006EDU). The package includes binding, equilibration, wash, and elution buffers. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0016EDU_0921121040480_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0016EDU_0921121040480_en.html 1155 <b>Refill contains sufficient buffers for 8 student workstations (2&ndash;4 students per workstation)</b> &nbsp; 1 0 1066 Binding buffer 1 1 0 1454 Column equilibration buffer 1 1 0 1461 Column wash buffer 1 1 0 1072 Elution buffer 1 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660016EDU&CartQuote=QT&quantity= 1711 false true false $73.75 true %2459.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660016EDU&AddToDefaultWishList=true&quantity= true false true 41116100 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660016EDU&quantity= 1660016EDU
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HIC Chromatography Columns and Caps

1660413EDU
Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only

1660413EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml 2 1660413EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlba8 2017-11-14 07:07:24 true true internet/product_SKU jthvlba8 templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml LSE 2019-04-06 07:35:42 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2019-04-06 07:35:42 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml 1660413edu-hic-chromatography-columns-caps 1660413EDU HIC Chromatography Columns and Caps HIC Chromatography Columns and Caps Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0413EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0413EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0413EDU_feature.jpg HIC Chromatography Columns and Caps #166-0413EDU RepEx HIC Chromatography Columns and Caps #166-0413EDU Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only 1660413, 1660413EDU, hic, chromatography, columns, and, caps, refill, hydrophobic, interaction, column, biotechnology, explorer, classroom, science, education, products 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 <p>Hydrophobic Interaction Chromatography Columns and Caps, package of eight, are for use with Biotechnology Explorer&trade; classroom kits. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0413EDU_0921121040426_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0413EDU_0921121040426_en.html 2728 HIC columns 8 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660413EDU&CartQuote=QT&quantity= 1711 false true false $52.50 true %2442.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660413EDU&AddToDefaultWishList=true&quantity= true false true 12161500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660413EDU&quantity= 1660413EDU
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Poly-Prep Columns

7311550EDU
Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only

7311550EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/731-1550EDU_0921121032943.xml 3 7311550EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbj8 2017-11-14 07:07:16 true true internet/product_SKU jthvlbj8 templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/731-1550EDU_0921121032943.xml LSE 2019-04-06 07:36:06 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2019-04-06 07:36:06 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/731-1550EDU_0921121032943.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/731-1550EDU_0921121032943.xml 7311550edu-poly-prep-columns 7311550EDU Poly-Prep Columns Poly-Prep Columns Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/731-1550EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/731-1550EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/731-1550EDU_feature.jpg Poly-Prep Columns #731-1550EDU RepEx Poly-Prep Columns #731-1550EDU Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only 7311550, 7311550EDU, poly-prep, columns, conical, empty, polypropylene, column, small-scale, gravity-flow, purification, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Chromatography N 0 <p>Poly-Prep<sup>&reg;</sup> Columns are conical 0.8 x 4 cm empty polypropylene columns, 9 cm high, with a 2 ml bed volume and a sample volume of 10 ml. They are sold as a package of 50. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 731-1550EDU_0921121032943_en.html /webroot/web/html/lse/products/SKUs/autopopulated//731-1550EDU_0921121032943_en.html 2709 Poly-Prep<sup>&reg;</sup> columns, empty, 0.8 x 4 cm 50 1 0 7317005 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-7005_0921120638974.xml 7311555 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-1555_0921120639354.xml 1560131 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7311550EDU&CartQuote=QT&quantity= 1712 false true false $96.00 true %2476.80 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7311550EDU&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7311550EDU&quantity= 7311550EDU
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Macro-Prep HIC Support

1560080EDU
100 ml, weakly hydrophobic methyl chromatography support; education use only

1560080EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml 4 1560080EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jthvlbfg 2017-11-14 07:07:15 true true internet/product_SKU jthvlbfg templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml LSE 2019-04-06 07:35:56 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2019-04-06 07:35:56 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml 1560080edu-macro-prep-hic-support 1560080EDU Macro-Prep HIC Support Macro-Prep HIC Support 100 ml, weakly hydrophobic methyl chromatography support; education use only 100 ml, weakly hydrophobic methyl chromatography support; education use only 100 ml, weakly hydrophobic methyl chromatography support; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/156-0080EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/156-0080EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/156-0080EDU_feature.jpg Macro-Prep HIC Support #156-0080EDU RepEx Macro-Prep HIC Support #156-0080EDU 100 ml, weakly hydrophobic methyl chromatography support; education use only 1560080, 1560080EDU, macro-prep, methyl, hic, support, hydrophobic, interaction, chromatography, media, medium, resin, protein, separation, purification, 50um, 50, particle, size, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Chromatography N 0 <p>Macro-Prep<sup>&reg;</sup> HIC Support, 100 ml, is a weakly hydrophobic methyl chromatography support. The product is for use with the Green Fluorescent Protein Chromatography Kit (#166-0005EDU) and the Secrets of the Rainforest&trade; Kit (#166-0006EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 156-0080EDU_0921121032560_en.html /webroot/web/html/lse/products/SKUs/autopopulated//156-0080EDU_0921121032560_en.html 2708 Macro-Prep<sup>&reg;</sup> HIC support, 100 ml 1 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560080EDU&CartQuote=QT&quantity= 1712 false true false $444.00 true %24355.20 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560080EDU&AddToDefaultWishList=true&quantity= true false true 12000000 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560080EDU&quantity= 1560080EDU
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Number Description Options
3053 Green Fluorescent Protein Chromatography Kit, Biotechnology Explorer, Quick Guide Click to download
4006099 Instruction Manual, Green Fluorescent Protein (GFP) Purification Kit (pGLO Transformation Module 2), Biotechnology Explorer, Rev C Please login to download this document

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1385504243813 US templatedata/internet/generic/data/en_US/GNL/page_generic_footer_b2c_1385505294_US.xml page_generic_footer_b2c /default/main/bio-rad/WORKAREA/default jc7v8okm 2017-10-27 13:13:40 true Done jc7v8okm templatedata/internet/generic/data/en_US/GNL/page_generic_footer_b2c_1385505294_US.xml GNL N /default/main/bio-rad en_US 11/27/23 02:18 PM 1539210190090 2018-12-28 22:30:28 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/en_US/GNL/page_generic_footer_b2c_1385505294_US.xml footer-page 1 1385504243813 page_generic_footer_b2c <div class="links"> <h4>Support</h4> <ul> <li><a class="msdsLink wtclass linkgeneration" href="/_locale/literature-library">MSDS</a></li> <li><a class="cofaLink wtclass linkgeneration" href="/_locale/_defaultVerticalUrl/support/certificate-of-analysis">Certificate of Analysis</a></li> <li><a class="qcinsertsLink wtclass" href="http://myeinserts.qcnet.com/" target="_blank" rel="noopener noreferrer">QC Inserts</a></li> <li><a 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