iScript cDNA Synthesis Kits

iScript Reverse Transcription Reagents feature an RNase H+ MMLV reverse transcriptase plus an RNase inhibitor for reliable yields. Choose the best cDNA synthesis kit for your application, with formulations optimized for ease, yield, flexibility, fidelity, or gDNA clearance.

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iScript cDNA Synthesis Kits

	iScript Reverse Transcription Supermix for RT-qPCR	iScript gDNA Clear cDNA Synthesis Kit	iScript Advanced cDNA Synthesis Kit for RT-qPCR	iScript Select cDNA Synthesis Kit	iScript cDNA Synthesis Kit
Features	Minimum reaction setup time	Effective gDNA removal before reverse transcription	Maximum RNA input for high cDNA yields	Flexible priming options and high-fidelity	Reliable value solution
Applications	Gene expression RNA quantification	Gene expression RNA quantification	Gene expression RNA quantification	Cloning Sequencing and NGS Gene expression RNA quantification	Gene expression RNA quantification
Total Input RNA Range	1 µg–1 pg	1 µg–1 pg	7.5 µg–100 fg	1 µg–1 pg	1 µg–100 fg
Kit Format	1 tube	3 tubes	2 tubes	5 tubes	2 tubes
Kit Contents	5x iScript RT Supermix No-RT control supermix	5x iScript RT Supermix No-RT control supermix DNase DNase buffer	iScript Reverse Transcriptase 5x iScript Advanced Reaction Mix	iScript Reverse Transcriptase 5x iScript Reaction Mix 3 priming options	iScript Reverse Transcriptase 5x iScript Reaction Mix
Time to Produce cDNA	26 min	36 min	21 min	15–65 min	26 min
RNase H+ Activity	✓	✓	✓	✓	✓
Documents	Manual Brochure	Manual Brochure	Manual Brochure	Manual Brochure	Manual

iScript Explore One-Step RT and PreAmp Kit

Reverse transcription and unbiased preamplification completed in a single step
Highly sensitive target detection
Effective genomic DNA clearance

Learn More

Resources

Understanding Reverse Transcription

This interactive tutorial provides an introduction to reverse transcription basics, an explanation of how different reagents work, and a protocol for comparing the results with different reverse transcription reagents.

Reagent Comparison Guide for Real-Time PCR

(PDF 3 MB)
Amplification Reagents and Plastics Brochure

(PDF 6.1 MB)
iScript Reverse Transcription Kits Flier

(PDF 208 KB)

Videos

Reverse Transcription: the Pros and Cons of Oligo-dT Priming

How RNase H+ Works

Optimal Blend Priming

Following the MIQE Guidelines for RT-qPCR Experiments

Applications & Technologies

qPCR/Real-Time PCR Reagents

Guidelines for selecting the appropriate reagents for your real-time PCR-based application
What is Gene Expression Analysis?

An introduction to gene regulation mechanisms, methods for gene expression profiling, and quantitation techniques including real-time PCR
lncRNA RT-qPCR Workflow

This innovative workflow enables highly sensitive detection of lncRNAs and helps to overcome challenges associated with lncRNA expression analysis
Real-Time PCR Doctor

Having trouble with real-time PCR experiments? The Real-Time PCR Doctor can help you diagnose and correct common qPCR problems

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