Are you having trouble with your real-time PCR experiment? Do you need help diagnosing your experiment? The Real-Time PCR Doctor is here to help. Unexpected fluorescence data are symptomatic of problems with your real-time PCR reaction components or amplification protocol. Click one of the symptoms below to learn about possible causes and treatments.
Related Topics: What Is Real-Time PCR?, How Real-Time PCR Works, and qPCR Assay Design and Optimization.
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If you don't see your symptom listed above, please contact technical support at 1-800-424-6723, option 2.
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