iScript™ Explore One-Step RT and PreAmp Kit

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iScriptExplore One-Step RT and PreAmp Kit

Overview

Preamplification Does Not Introduce Bias

Unbiased Amplification

Preamplification does not introduce bias. Potential for bias was evaluated using External RNA Consortium Controls (ERCC) controls, which are RNA transcripts of a known copy number. ERCC concentrations from single copy to 16,000 copies were used to assess bias across a wide range of transcript concentrations. RNA samples were reverse transcribed using iScript™ Advanced cDNA Synthesis Kit or reverse transcribed and preamplified using iScript Explore One-Step RT and PreAmp Kit. The R2 value of 0.952 indicates a high correlation between samples with and without preamplification, demonstrating that preamplification does not introduce bias.

Highly Sensitive and Robust Target Detection at Low Copy Number

data sensitivity
Melt curve

1 copy (average) input
iScript Explore: 20 of 20 positive
iScript Advanced: 2 of 20 positive
Competitor T: 1 of 20 positive

iScript™ Explore One-Step RT and PreAmp Kits enable sensitive detection of rare transcripts. The unique single-step RT and preamplification reaction allows for reproducible detection of targets down to the single-copy level.

RNA samples containing a single copy of target RNA were reverse transcribed using iScript Advanced cDNA Synthesis Kit or Competitor T Reverse Transcriptase, or reverse transcribed and preamplified using iScript Explore One-Step RT and PreAmp Kit. Resulting cDNA samples were run in 20 replicate qPCR reactions to determine target detection. Only the iScript Explore One-Step RT and PreAmp Kit provides consistent detection of the rare target in each qPCR reaction (20 of 20 reactions were positive). Rare events were not reliably detected in the absence of preamplification (resulting samples from iScript Advanced cDNA Synthesis Kit and Competitor T Reverse Transcriptase). Melt curve analysis demonstrates a single peak in all positive samples, suggesting that a single, specific product was produced in all cases.

PreAmp Control Assay Validates Preamplification Performance

The PreAmp Control Assay validates the performance of the preamplification reaction when using the iScript™ Explore One-Step RT and PreAmp Kit. This control provides confidence that preamplification was completed as expected.

This control is included on all PrimePCR lncRNA Predesigned Arrays and can also be included in any custom designed PrimePCR Array. When using iScript Explore Kit, PAQ1, but not PAQ2, is amplified during the preamplification reaction. During the qPCR reaction, both PAQ1 and PAQ2 are amplified. The delta Cq between the preamplified and the non-preamplified assays should approximately equal the number of preamplification cycles run.

PreAmp Control

PrimePCR PreAmp Control demonstrates preamplification efficacy: Fourteen rounds of cDNA synthesis and preamplification were performed using the iScript Explore One-Step RT and PreAmp Kit followed by qPCR. Preamplification efficacy was determined by analyzing the delta Cq between the PAQ1 and PAQ2 assays. The delta Cq between these two reactions was equivalent to the number of preamplification cycles performed.

Effective gDNA Removal Prior to One-Step RT and Preamplification

Effective gDNA Removal

iScript™ Explore One-Step RT and PreAmp Kit effectively clears contaminating genomic DNA (gDNA). HeLa RNA was purified using the Aurum Total RNA Mini Kit without gDNA clearance. RNA (10 ng) was processed using iScript Explore One-Step RT and PreAmp Kit with (red traces) and without (green traces) the gDNA clearance option for 14 preamplification cycles. Levels of residual gDNA were assessed using the PrimePCR™ gDNA Contamination Control Assay. Samples treated with DNase showed no detectable expression, illustrating complete gDNA clearance.

Exclusive Sso7d Fusion Polymerase Technology to Empower Your PCR

Sso7d, a 7 kD protein obtained from Sulfolobus solfataricus, binds to dsDNA without any sequence specificity.

Sso7d is covalently linked to a DNA polymerase and is able to stabilize the polymerase-template complex without compromising the structural integrity, thermal stability, or catalytic activity of the enzyme.

The unique structure of this Sso7d fusion polymerase provides several significant advantages over ordinary DNA polymerase:

  • Increased processivity
  • Effective amplification in GC-rich regions or strong secondary structure
  • Efficient amplification of longer products
  • Superior tolerance to PCR inhibitors
Sso7D Technology
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iScript Explore One-Step RT and PreAmp Kit is a highly sensitive reagent that can be used to generate preamplified cDNA directly from RNA in a single step. This kit is well suited for detecting many targets from a limited sample or for reliable detection of targets expressed at low levels. The patented Sso7d fusion protein provides unbiased target-specific preamplification of up to 100 targets. The kit also integrates an effective genomic DNA removal step, helping ensure only the transcripts of interest are detected by qPCR.

Features and Benefits

  • One-step reverse transcription and preamplification — the first and only kit that eliminates protocol steps by generating preamplified cDNA from RNA in a single step
  • Unbiased preamplification — can reliably profile panels of targets
  • High sensitivity — enables reliable detection of genes expressed at low levels
  • Effective genomic DNA (gDNA) removal — provides confidence that results are only for the transcript of interest

Simple Workflow

Simply create gDNA-free, preamplified cDNA by combining the reagents in this kit with RNA sample and PreAmp Assays. This kit contains all of the reagents needed to easily create an unbiased 1,000-fold amplification of cDNA.

iScript Explore

Preamplification of Targets without Bias

Preamplification is a powerful tool to enable highly sensitive detection of a large number of targets from a limited sample. The combination of proprietary buffers and the Sso7d fusion technology enables the iScript Explore One-Step RT and PreAmp Kit to generate unbiased preamplification reactions regardless of the amount of target present.

Importance of Addressing gDNA Contamination in RT-qPCR Gene Expression

Contaminating gDNA in RNA samples can be amplified in experiments that use exonic primers, biasing the results for the transcript of interest. This problem is especially acute for low expressing genes, such as lncRNAs — even a few copies of contaminating gDNA can shift Cq values dramatically. The iScript Explore One-Step RT and PreAmp Kit addresses the issue with a specially formulated DNase that effectively eliminates gDNA from purified RNA.

Applications and Uses

  • For reliable detection of targets expressed at low levels, including
  • Facilitates screening a large number of genes from limited samples, especially powerful when using PrimePCR Arrays
  • Ideal for single cell RT-qPCR workflows
  • For highly sensitive detection when working with limited sample, including laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded (FFPE) tissues

Preamplification Product Comparison

Choose the preamplification product that best suits your needs.

Products Description Key Benefits
iScript Explore One-Step RT and PreAmp Kit cDNA synthesis and preamplification done in a single step
  • RNA is used as sample input
  • Reverse transcription done using gene specific priming
  • Unbiased preamplification
  • Protocol minimizes hands-on time
  • Kit integrates an effective gDNA digestion step
SsoAdvanced™ PreAmp Supermix cDNA synthesis and preamplification are performed using separate kits in two distinct steps
  • Perform cDNA synthesis using any of the iScript Reverse Transcription Reagents
  • cDNA is used as sample input for preamplification reaction
  • Unbiased preamplification
  • Flexibility with cDNA synthesis method of choice
  • Archive cDNA for future studies of different genes

Related Categories

Supporting Documents

Online Resources

Shelf life 12 months (from shipping date)
Storage Store DNase buffer at 4°C upon receipt. Store all other kit components at –20°C.
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iScript™ Explore One-Step RT and PreAmp Kit, 50 x 50 µl rxns

12004856
50 x 50 µl reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water

12004856 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12004856_1499119891.xml 12004856 07/03/17 01:42 PM en_US true internet/product_SKU 2018-03-16 04:17:55 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12004856_1499119891.xml /default/main/bio-rad/WORKAREA/default jddgmjq5 2017-11-14 07:07:35 true jddgmjq5 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12004856_1499119891.xml LSR N /default/main/bio-rad en 07/03/27 03:05 PM 1520560198304 2018-03-16 04:17:55 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/12004856_1499119891.xml 12004856-iscript-explore-one-step-rt-preamp-kit-50-x-50-ul-rxns 1 12004856 iScript Explore One-Step RT and PreAmp Kit iScript&trade; Explore One-Step RT and PreAmp Kit, 50 x 50 &micro;l rxns 50 x 50 &micro;l reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/12004856-tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/12004856-view.jpg preview Karen Moss iScript Explore One-Step RT & PreAmp Kit 12004856, iscript[*4], explore, one-step, 1-step, rt, preamp, preamplification, preamplified, cdna, synthesis, cdna synthesis, rt-qpcr, reverse transcription, reverse transcriptase, transcriptase, first strand, complimentary, gene expression, real, time, qpcr, real-time, pcr, sso7d, ssoadvanced, so advanced, advanced, fusion polymerase, rna, lncrna, long non coding rna, long non-coding rna, long noncoding rna, long ncrna 07/03/17 01:42 PM 07/03/27 03:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the iScript Explore One-Step RT and PreAmp Kit to generate preamplified cDNA directly from RNA in a single step. This kit is suited for detecting many targets from a limited sample or for reliable detection of targets expressed at low levels. The patented Sso7d fusion protein provides unbiased target-specific preamplification of up to 100 targets. The kit also integrates an effective genomic DNA removal step, helping ensure only the transcripts of interest are detected by qPCR.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>One-step reverse transcription and preamplification &mdash; the first and only kit that eliminates protocol steps by generating preamplified cDNA from RNA in a single step</li> <li><strong><a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unbiased-preamplification-desc.html&quot;);">Unbiased preamplification</a></strong> &mdash; can reliably profile panels of targets</li> <li><strong><a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/highly-sensitive-desc.html&quot;);">High sensitivity</a></strong> &mdash;<strong> </strong>enables reliable detection of genes expressed at low levels</li> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only"><strong><a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/preamp-control-assay-desc.html&quot;);">Preamplification control</a></strong> &mdash; preamplification efficacy can be easily assessed when used with PrimePCR&trade; long noncoding RNA (lncRNA) Arrays or custom PrimePCR Plates</li> <li><strong><a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/effective-gdna-removal-desc.html&quot;);">Effective genomic DNA (gDNA) removal</a></strong> &mdash; provides confidence that results are only for the transcript of interest</li> </ul> <h2 class="descHeader">Simple Workflow</h2> <p>Simply create gDNA-free, preamplified cDNA by combining the reagents in this kit with RNA sample and PreAmp Assays. This kit contains all of the reagents needed to easily create an unbiased 1,000-fold amplification of cDNA.</p> <p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/iscript-explore-workflow4.png" alt="iScript Explore" width="598px" height="178px" /></p> <h2 class="descHeader">Preamplification of Targets without Bias</h2> <p>Preamplification is a powerful tool to enable highly sensitive detection of a large number of targets from a limited sample. The combination of proprietary buffers and the <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sso7d-technology-desc.html&quot;);">Sso7d fusion technology</a> enables the iScript Explore One-Step RT and PreAmp Kit to generate unbiased preamplification reactions regardless of the amount of target present.</p> <h2 class="descHeader">Importance of Addressing gDNA Contamination in RT-qPCR Gene Expression</h2> <p>Contaminating gDNA in RNA samples can be amplified in experiments that use exonic primers, biasing the results for the transcript of interest. This problem is especially acute for low expressing genes, such as <span class="forcebreaks">lncRNAs &mdash;</span> even a few copies of contaminating gDNA can shift Cq values dramatically. The iScript Explore One-Step RT and PreAmp Kit addresses the issue with a specially formulated DNase that effectively eliminates gDNA from purified RNA.</p> <h2 class="descHeader">Applications and Uses</h2> <ul> <li>For reliable detection of targets expressed at low levels, including <span class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only"><a href="/en-us/category/lncrna-rt-qpcr-workflow?ID=OUJ92R15">lncRNAs</a></span> <span class="hidden US-exclude CA-exclude AT-exclude BE-exclude CZ-exclude DK-exclude FI-exclude FR-exclude DE-exclude HU-exclude IL-exclude IT-exclude JP-exclude KR-exclude LU-exclude NO-exclude PL-exclude PT-exclude ES-exclude NL-exclude SE-exclude CH-oexclude UK-exclude AU-exclude NZ-exclude SG-exclude CN-exclude TW-exclude HK-exclude MO-exclude TH-exclude ZA-exclude">lncRNAs</span></li> <li>Facilitates screening a large number of genes from limited samples, especially powerful when using PrimePCR Arrays</li> <li>Ideal for single cell RT-qPCR workflows</li> <li>For highly sensitive detection when working with limited sample, including laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded (FFPE) tissues</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td><strong>Kit Size</strong></td> <td style="text-align: center;"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td>50 x 50 &micro;l reactions</td> <td style="text-align: center;">12004856</td> </tr> <tr> <td>250 x 50 &micro;l reactions</td> <td style="text-align: center;"><a href="/en-us/sku/12004856-iscript-explore-one-step-rt-preamp-kit-250-x-50-ul-rxns?ID=17002826">17002826</a></td> </tr> </tbody> </table> <h2 class="descHeader">Preamplification Product Comparison</h2> <p>Choose the preamplification product that best suits your needs.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="248"><strong>Products</strong></td> <td style="text-align: left;" width="248"><strong>Description</strong></td> <td style="text-align: left;" width="248"><strong>Key Benefits</strong></td> </tr> <tr class="pd_colorbackground"> <td width="248" align="left" valign="top"><a href="/en-us/product/iscript-explore-one-step-rt-preamp-kit?ID=OSMVGR4VY">iScript Explore One-Step RT and PreAmp Kit</a></td> <td width="248" align="left" valign="top">cDNA synthesis and preamplification done in a single step <ul style="font-size: 11px;"> <li>RNA is used as sample input</li> <li>Reverse transcription done using gene specific priming</li> </ul> </td> <td width="248" align="left" valign="top"> <ul style="font-size: 11px;"> <li>Unbiased preamplification</li> <li>Protocol minimizes hands-on time</li> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only">Compatible with PrimePCR PreAmp Control (PAQ)</li> <li>Kit integrates an effective gDNA digestion step</li> </ul> </td> </tr> <tr> <td width="248" align="left" valign="top"><a href="/en-us/product/preamplification-supermix">SsoAdvanced&trade; PreAmp Supermix</a></td> <td width="248" align="left" valign="top">cDNA synthesis and preamplification are performed using separate kits in two distinct steps <ul style="font-size: 11px;"> <li>Perform cDNA synthesis using any of the iScript Reverse Transcription Reagents</li> <li>cDNA is used as sample input for preamplification reaction</li> </ul> </td> <td width="248" align="left" valign="top"> <ul style="font-size: 11px;"> <li>Unbiased preamplification</li> <li>Flexibility with cDNA synthesis method of choice</li> <li>Archive cDNA for future studies of different genes</li> </ul> </td> </tr> </tbody> </table> <h2 class="descHeader">Related Categories</h2> <ul> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only"><a href="/en-us/category/lncrna-rt-qpcr-workflow?ID=OUJ92R15">lncRNA RT-qPCR Workflow</a></li> <li><a href="/en-us/category/real-time-pcr-supermixes-kits">Real-Time PCR Supermixes</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only"><a href="/en-us/product/primepcr-pcr-primers-assays-arrays">PrimePCR PCR Primers &amp; Assays</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer">Brochure</a></li> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only">Simplified Long Noncoding RNA Discovery <a href="/webroot/web/pdf/lsr/literature/Bulletin_6984.pdf" target="_blank" rel="noopener noreferrer">Brochure</a></li> <li>iScript Explore One-Step RT and PreAmp Kit <a href="/webroot/web/pdf/lsr/literature/10000086144.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/what-real-time-pcr-qpcr">What is Real-Time PCR (qPCR)?</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 12004856-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/12004856-desc.html Shelf life 2149 12 months (from shipping date) 1 0 Storage 2198 Store DNase buffer at 4&deg;C upon receipt. 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iScript™ Explore One-Step RT and PreAmp Kit, 250 x 50 µl rxns

17002826
250 x 50 µl reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water

17002826 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/17002826_1499120772.xml 17002826 07/03/17 03:12 PM en_US true internet/product_SKU 2018-03-16 04:17:55 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/17002826_1499120772.xml /default/main/bio-rad/WORKAREA/default jddgmjpv 2017-11-14 07:07:35 true jddgmjpv templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/17002826_1499120772.xml LSR N /default/main/bio-rad en 07/03/27 03:14 PM 1520560198350 2018-03-16 04:17:55 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/17002826_1499120772.xml 17002826-iscript-explore-one-step-rt-preamp-kit-250-x-50-ul-rxns 1 17002826 iScript Explore One-Step RT and PreAmp Kit iScript&trade; Explore One-Step RT and PreAmp Kit, 250 x 50 &micro;l rxns 250 x 50 &micro;l reactions, contains iScript Advanced Reverse Transcriptase, 2x PreAmp Mix (dNTPs, Sso7d fusion polymerase, salts, enhancers, stabilizers, and other proprietary components), reaction booster, DNase, DNase buffer, nuclease-free water /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/17002826-tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/17002826-view.jpg preview Karen Moss iScript Explore One-Step RT & PreAmp Kit 17002826, iscript[*4], explore, one-step, 1-step, rt, preamp, preamplification, preamplified, cdna, synthesis, cdna synthesis, rt-qpcr, reverse transcription, reverse transcriptase, transcriptase, first strand, complimentary, gene expression, real, time, qpcr, real-time, pcr, sso7d, ssoadvanced, so advanced, advanced, fusion polymerase, rna, lncrna, long non coding rna, long non-coding rna, long noncoding rna, long ncrna 07/03/17 03:12 PM 07/03/27 03:14 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the iScript Explore One-Step RT and PreAmp Kit to generate preamplified cDNA directly from RNA in a single step. This kit is well suited for detecting many targets from a limited sample or for reliable detection of targets expressed at low levels. The patented Sso7d fusion protein provides unbiased target specific preamplification of up to 100 targets. The kit also integrates an effective genomic DNA removal digestion step, helping ensure only the transcripts of interest are detected by qPCR.</p> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>One-step reverse transcription and preamplification &mdash; the first and only kit that eliminates protocol steps by generating preamplified cDNA from RNA in a single step</li> <li><strong><a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/unbiased-preamplification-desc.html&quot;);">Unbiased preamplification</a></strong> &mdash; can reliably profile panels of targets</li> <li><strong><a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/highly-sensitive-desc.html&quot;);">High sensitivity</a></strong> &mdash;<strong> </strong>enables reliable detection of genes expressed at low levels</li> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only"><strong><a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/preamp-control-assay-desc.html&quot;);">Preamplification control</a></strong> &mdash; preamplification efficacy can be easily assessed when used with PrimePCR&trade; long noncoding RNA (lncRNA) Arrays or custom PrimePCR Plates</li> <li><strong><a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/effective-gdna-removal-desc.html&quot;);">Effective genomic DNA (gDNA) removal</a></strong> &mdash; provides confidence that results are only for the transcript of interest</li> </ul> <h2 class="descHeader">Simple Workflow</h2> <p>Simply create gDNA-free, preamplified cDNA by combining the reagents in this kit with RNA sample and PreAmp Assays. This kit contains all of the reagents needed to easily create an unbiased 1,000-fold amplification of cDNA.</p> <p><img src="/webroot/web/images/lsr/products/amplification_pcr/product_detail/global/iscript-explore-workflow4.png" alt="iScript Explore" width="598px" height="178px" /></p> <h2 class="descHeader">Preamplification of Targets without Bias</h2> <p>Preamplification is a powerful tool to enable highly sensitive detection of a large number of targets from a limited sample. The combination of proprietary buffers and the <a href="javascript:openAjaxOverlay(&quot;/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sso7d-technology-desc.html&quot;);">Sso7d fusion technology</a> enables the iScript Explore One-Step RT and PreAmp Kit to generate unbiased preamplification reactions regardless of the amount of target present.</p> <h2 class="descHeader">Importance of Addressing gDNA Contamination in RT-qPCR Gene Expression</h2> <p>Contaminating gDNA in RNA samples can be amplified in RT-qPCR experiments that use exonic primers, biasing the results for the transcript of interest. This problem is especially acute for low expressing genes, such as <span class="forcebreaks">lncRNAs &mdash;</span> even a few copies of contaminating gDNA can shift Cq values dramatically. The iScript Explore One-Step RT and PreAmp Kit addresses the issue with a specially formulated DNase that effectively eliminates gDNA from purified RNA.</p> <h2 class="descHeader">Applications and Uses</h2> <ul> <li>For reliable detection of targets expressed at low levels, including <span class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only"><a href="/en-us/category/lncrna-rt-qpcr-workflow?ID=OUJ92R15">lncRNAs</a></span> <span class="hidden US-exclude CA-exclude AT-exclude BE-exclude CZ-exclude DK-exclude FI-exclude FR-exclude DE-exclude HU-exclude IL-exclude IT-exclude JP-exclude KR-exclude LU-exclude NO-exclude PL-exclude PT-exclude ES-exclude NL-exclude SE-exclude CH-oexclude UK-exclude AU-exclude NZ-exclude SG-exclude CN-exclude TW-exclude HK-exclude MO-exclude TH-exclude ZA-exclude">lncRNAs</span></li> <li>Facilitates screening a large number of genes from limited samples, especially powerful when using PrimePCR Arrays</li> <li>Ideal for single cell RT-qPCR workflows</li> <li>For highly sensitive detection when working with limited sample, including laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded (FFPE) tissues</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td><strong>Kit Size</strong></td> <td style="text-align: center;"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td>50 x 50 &micro;l reactions</td> <td style="text-align: center;"><a href="/en-us/sku/12004856-iscript-explore-one-step-rt-preamp-kit-50-x-50-ul-rxns?ID=12004856">12004856</a></td> </tr> <tr> <td>250 x 50 &micro;l reactions</td> <td style="text-align: center;">17002826</td> </tr> </tbody> </table> <h2 class="descHeader">Preamplification Product Comparison</h2> <p>Choose the preamplification product that best suits your needs.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="248"><strong>Products</strong></td> <td style="text-align: left;" width="248"><strong>Description</strong></td> <td style="text-align: left;" width="248"><strong>Key Benefits</strong></td> </tr> <tr class="pd_colorbackground"> <td width="248" align="left" valign="top"><a href="/en-us/product/iscript-explore-one-step-rt-preamp-kit?ID=OSMVGR4VY">iScript Explore One-Step RT and PreAmp Kit</a></td> <td width="248" align="left" valign="top">cDNA synthesis and preamplification done in a single step <ul> <li>RNA is used as sample input</li> <li>Reverse transcription done using gene specific priming</li> </ul> </td> <td width="248" align="left" valign="top"> <ul> <li>Unbiased preamplification</li> <li>Protocol minimizes hands-on time</li> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only">Compatible with PrimePCR PreAmp Control (PAQ)</li> <li>Kit integrates an effective gDNA digestion step</li> </ul> </td> </tr> <tr> <td width="248" align="left" valign="top"><a href="/en-us/product/preamplification-supermix">SsoAdvanced&trade; PreAmp Supermix</a></td> <td width="248" align="left" valign="top">cDNA synthesis and preamplification are performed using separate kits in two distinct steps <ul> <li>Perform cDNA synthesis using any of the iScript Reverse Transcription Reagents</li> <li>cDNA is used as sample input for preamplification reaction</li> </ul> </td> <td width="248" align="left" valign="top"> <ul> <li>Unbiased preamplification</li> <li>Flexibility with cDNA synthesis method of choice</li> <li>Archive cDNA for future studies of different genes</li> </ul> </td> </tr> </tbody> </table> <h2 class="descHeader">Related Categories</h2> <ul> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only"><a href="/en-us/category/lncrna-rt-qpcr-workflow?ID=OUJ92R15">lncRNA RT-qPCR Workflow</a></li> <li><a href="/en-us/category/real-time-pcr-supermixes-kits">Real-Time PCR Supermixes</a></li> <li><a href="/en-us/category/real-time-pcr-detection-systems">Real-Time PCR Detection Systems</a></li> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only"><a href="/en-us/product/primepcr-pcr-primers-assays-arrays">PrimePCR PCR Primers &amp; Assays</a></li> <li><a href="/en-us/category/pcr-plastic-consumables">PCR Plastic Consumables</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Amplification Reagents and Plastics <a href="/webroot/web/pdf/lsr/literature/Bulletin_6090.pdf" target="_blank" rel="noopener noreferrer">Brochure</a></li> <li class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IL-only IT-only JP-only KR-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only">Simplified Long Noncoding RNA Discovery <a href="/webroot/web/pdf/lsr/literature/Bulletin_6984.pdf" target="_blank" rel="noopener noreferrer">Brochure</a></li> <li>iScript Explore One-Step RT and PreAmp Kit <a href="/webroot/web/pdf/lsr/literature/10000086144.pdf" target="_blank" rel="noopener noreferrer">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/what-real-time-pcr-qpcr">What is Real-Time PCR (qPCR)?</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 17002826-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/17002826-desc.html Shelf life 2149 12 months (after shipping date) 1 0 Storage 2198 Store DNase buffer at 4&deg;C upon receipt. Store all other kit components at <br/>&ndash;20&deg;C. 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=17002826&CartQuote=QT&quantity= false true false $3,930.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=17002826&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=17002826&quantity= 17002826
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10000086144 Instruction Manual, iScript™ Explore One-Step RT and PreAmp Kit Click to download
6984 Simplified Long Noncoding RNA Discovery Brochure Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]

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