QX200™ Droplet Digital™ PCR (ddPCR™) SystemFor analyzing up to 96 samples
QX200™ AutoDG™ Droplet Digital™ PCR SystemFor high-throughput analysis
ddPCR SupermixesFor sensitive DNA target detection
PrimePCR™ ddPCR™ AssaysExperimentally validated primer pairs
Digital PCR Library Quantification KitsFor quantifying sequencing libraries
Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification. It is particularly useful for low abundance targets, targets in complex backgrounds, allelic variants (SNPs), and for monitoring subtle changes in target levels that cannot be detected with real-time PCR.
Based on water-emulsion droplet technology, Droplet Digital PCR fractionates a DNA sample in 20,000 droplets. PCR amplification of the template subsequently occurs in each individual droplet, and counting the positive droplets gives precise, absolute target quantification.
Read more about Droplet Digital PCR technology.
DNA sample with target sequence is partitioned into droplets
Target and background DNA are randomly distributed among 20,000 droplets
Target sequence is amplified by end-point PCR in each droplet
Positive droplets are counted to give precise quantification of target sequences in sample
Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves
Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR
High throughput — simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps
Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR
Copy number variation (CNV) Accurate CNV quantification in single wells
Next generation sequencingAmplification and accurate quantification of NGS libraries with validation of sequencing results
Detection of rare sequences in cancer and diseaseDetection of mutations and viral load below levels detectable by current tests
Gene expression analysisDetection of rare mRNAs and miRNAs with rapid turnover
Pathogen detection and microbiome analysisViral load and pathogen detection in organ transplants, soil, and food
Single cell analysisHigh sensitivity make it a robust tool for single-cell analyses
Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments.
QX200 ddPCR SystemThe QX200 System includes Droplet Generator, Droplet Reader, and QuantaSoft™ Software.
QX200 AutoDG Droplet Digital PCR SystemDroplet generation can be automated for high throughput using the QX200 AutoDG Droplet Digital System.
C1000 Touch™ Thermal Cycler You can use the C1000 Touch Thermal Cycler with the 96–Deep Well Reaction Module to run the PCR step.
Digital PCR SupermixesddPCR Kits are offered in two chemistries, for probes and EvaGreen
Digital PCR AssaysFully validated assays for hydrolysis probe–based and EvaGreen Assays
Digital PCR Library Quantification KitsLibrary Quantification Kits for Illumina and Ion Torrent NGS
Pierceable Foil Heat SealSecure sealing while enabling sample retrieval from selected wells
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