Droplet Digital PCR (ddPCR)

  •  

    In cell and gene therapy, there's no room for error.

     

Droplet Digital™ PCR (ddPCR™) is a breakthrough technology that provides ultrasensitive nucleic acid detection and absolute quantification. It is highly effective for resolving low abundance targets, such as allelic or structural variants, that are below the level of detection of other platforms.

With advanced multiplexing offerings, ddPCR technology maximizes the number of targets analyzed per well without sacrificing performance.

Featured PRODUCTQX600 Droplet Digital PCR System

Since its introduction, ddPCR technology has advanced critical fields of research through unmatched sensitivity, precision, and absolute quantitation. Now the QX600 System provides advanced, 6-color multiplexing to maximize the number of biomarkers interrogated per well, allowing the preservation of precious samples and reducing lab burden.

Start Your Journey

Digital PCR Assays & Kits

Image
Droplet Digital PCR (ddPCR) Assays and Kits

Bio-Rad offers a comprehensive portfolio of Digital PCR Assays and Kits for numerous applications including Mutation Detection, Copy Number Determination, Genome Edit Detection, Gene Expression, Expert Design Assays, Residual DNA Quantification and Library Quantification.

Explore ddPCR Assays & Kits

 
  • Image
    Droplet Digital PCR Assays

    Featured Product Digital PCR Assays

    Get the most out of your experiment with assays designed specifically for Droplet Digital PCR. Simply enter your target of interest or sequence and order a ddPCR Assay.

    Buy Digital PCR Assays

  • Image
    ddPCR-MSI-Kit

    NEW PRODUCT ddPCR Microsatellite Instability (MSI) Kit

    The only kit designed to analyze both plasma and FFPE tissue, it provides highly sensitive and reproducible results across all relevant cancer samples without the need for normal tissue. Automated analysis and a streamlined workflow make this the premier MSI solution.

    Empower Your Research

Digital PCR Systems, Software, and Consumables

ddpcr systems

Droplet Digital PCR Systems

Bio-Rad’s Droplet Digital PCR Systems provide ultrasensitive and absolute nucleic acid quantification.

QX Software for Digital PCR Systems

QX Software for Digital PCR Systems

QX Software is a family of powerful software packages for acquisition and analysis of your Droplet Digital PCR (ddPCR) data. When connected to a ddPCR System, QX Software provides all necessary functionality to create, run, and analyze experiments on your samples.

Learn More about QX Software for Digital PCR Systems »

Digital PCR Consumables and Reagents

Purchase supermixes, droplet generation oil, cartridges, gaskets, plates & seals, plus pipette tips, and more, designed for Droplet Digital PCR Systems.

ddPCR Consumables

QXDx Products for In Vitro Diagnostics (IVD)

Droplet Digital PCR Systems

QXDx AutoDG ddPCR System

IVD Consumables, Assays, and Kits

ddPCR Certified Kit Provider Partnership Program

Partnering with Bio-Rad for ddPCR Test Kit Development

Bio-Rad partners with test kit providers to develop Droplet Digital™ PCR (ddPCR) assays and diagnostic test kits. We help kit manufacturers to develop and commercialize IVD and registered ddPCR test kits using our Droplet Digital PCR platform. We can help you with assay design, in-licensing, co-development, and co-marketing opportunities.

Learn More

Droplet Digital PCR Technology

Based on water-emulsion droplet technology, Droplet Digital PCR fractionates a DNA sample in 20,000 droplets. PCR amplification of the template subsequently occurs in each individual droplet, and counting the positive droplets gives precise, absolute target quantification.

Read more about Droplet Digital PCR technology or learn how to transition your assay from qPCR to ddPCR.

  • DNA sample with target sequence is partitioned into droplets

    DNA sample with target sequence is partitioned into droplets

  • Target and background DNA are randomly distributed among 20,000 droplets

    Target and background DNA are randomly distributed among 20,000 droplets

  • Target sequence is amplified by end-point PCR in each droplet

    Target sequence is amplified by end-point PCR in each droplet

  • Positive droplets are counted to give a precise quantitation of target sequences in sample

    Positive droplets are counted to give precise quantification of target sequences in sample

Benefits of Droplet Digital PCR

Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves

Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR

Removal of PCR efficiency bias — error rates are reduced by removing the reliance on amplification efficiency of qPCR

Explore Absolute Quantification with Droplet Digital PCR, download the White Paper on Molecular Counting.

Droplet Digital PCR Applications

Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments.

Bio-Rad is the Trusted Choice for Digital PCR

When it comes to digital PCR, in particular Droplet Digital PCR (ddPCR), Bio-Rad stands as a trusted leader with a track record that speaks for itself. With over 10 years of continuous innovation in digital PCR, and with 8,000+ publications utilizing our ddPCR technology, Bio-Rad has established its reputation as a reliable and respected provider.

 
Image
Scientist looking at tablet and writing notes

8,000+ Publications and Counting

Browse the growing list of cited peer-reviewed journal articles in Droplet Digital PCR research.

Browse Publications

 

Over a Decade of Droplet Digital PCR Innovation and Breakthroughs

  • Image
    Bio-Rad Acquires QuantaLife and Digital PCR Technology
    2011

    Bio-Rad Acquires QuantaLife & Digital PCR Technology
    This next-generation PCR technology builds on the well-established method of amplifying DNA that is used in research laboratories around the world.
    Read the Press Release »

  • Image
    QX100 Droplet Digital PCR System
    2012

    QX100™ Droplet Digital™ PCR System
    Evaluating a treatment for AIDS. Analyzing archival cancer samples. Tracking the RNA of a mutated gene known to cause cancer.
    Read the Press Release »

  • Image
    Scientists using the QX100 Droplet Digital PCR System
    2012

    QX100 Droplet Digital PCR
    Bio-Rad releases QX100 Droplet Digital PCR Success Stories video, interviewing technicians from labs using Droplet Digital PCR system.
    Watch the Video

  • Image
    QX200 Droplet Digital PCR System
    2013

    Launch of the QX200™ Droplet Digital™ PCR System
    Providing absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.
    See the System »

  • Image
    Using Droplet Digital PCR to Diagnose Leukemia
    2013

    Using Droplet Digital PCR to Diagnose Leukemia
    Prof. Alec Morley, a pioneer in digital PCR, describes his use of Droplet Digital PCR (ddPCR) technology to advance the diagnosis and treatment of leukemia.
    Watch the Video

  • Image
    QX200 AutoDG Droplet Digital PCR System
    2014

    Launch of the QX200™ AutoDG Droplet Digital™ PCR System
    The AutoDG Instrument simplifies the Droplet Digital PCR workflow, making digital PCR both scalable and practical.
    See the System »

  • Image
    ddPCR Multiplex Mutation Screening Kit
    2014

    ddPCR Multiplex Mutation Screening Kit Launch
    Designed for rapid screening of several key cancer mutations in a single reaction with high precision and sensitivity.
    See the Kit »

  • Image
    Bio-Rad ddPCR Used in Published Medical Journal Article
    2014

    Bio-Rad ddPCR Used in Published Medical Journal Article
    A Droplet Digital PCR detection method for rare L1 insertions in tumors.
    Read the Article

  • Image
    Droplet Digital PCR PrimePCR Assay Kits for Spinal Muscular Atrophy Research
    2015

    Bio-Rad Introduces Droplet Digital™ PCR PrimePCR™ Assay Kits for Spinal Muscular Atrophy Research
    This release expands Bio-Rad’s offering of predesigned, fully wet-lab validated assays with kits for SMN1 and SMN2 copy number determination.
    Read the Press Release »

  • Image
    ddPCR Residual DNA Quantification Kits
    2015

    ddPCR Residual DNA Quantification Kits Launch
    Bio-Rad’s ddPCR Residual DNA Quantification Kits are ideal for highly precise quantification of HCD in complex bioprocess intermediates.
    See the Kits »

  • Image
    Droplet Digital PCR Proves Highly Reproducible at Identifying Viral RNA Mutations in Clinical Samples
    2015

    Droplet Digital PCR Proves Highly Reproducible at Identifying Viral RNA Mutations in Clinical Samples
    Bio-Rad’s Droplet Digital PCR (ddPCR) technology identifies viral RNA mutations in clinical samples with greater precision, sensitivity, and reproducibility than quantitative reverse-transcription PCR (RT-qPCR), according to a new study in the Journal of Virological Methods.
    Read the Press Release »

  • Image
    1-Step RT-ddPCR Advanced Kit for Probes
    2015

    1-Step RT-ddPCR Advanced Kit for Probes
    This digital PCR supermix kit enables absolute quantification of target RNA molecules in a one-step format.
    See the Kits »

  • Image
    CE IVD Mark
    2016

    The First Digital PCR Instrument with the CE IVD Mark
    Bio-Rad announces CE IVD marking of its QX200 Droplet Digital PCR System for use as an in vitro diagnostic.
    Read the Press Release »

  • Image
    Host Cell Kits and Multiplex Mutation Screening
    2016

    Launch of Host Cell Kits & Multiplex Mutation Screening
    New application allows precise and accurate cell counts of an edited or modified cell population with minimal input cells.
    Read the Bulletin »

  • Image
    Portfolio of Digital PCR Assays and Kits
    2016

    Bio-Rad Launches a Comprehensive Portfolio of Digital PCR Assays and Kits
    Applications including Mutation Detection, Copy Number Determination, Genome Edit Detection, Gene Expression, Expert Design Assays, Residual DNA Quantification, and Library Quantification.
    See the Kits »

  • Image
    QXDx Universal Kit for AutoDG ddPCR System
    2017

    Bio-Rad Launches QXDx™ Universal Kit for AutoDG ddPCR™ System
    Targeted for optimized use with the automated QXDx System, this kit enables users to complete every step of the automated Droplet Digital PCR in vitro diagnostics workflow with high efficiency.
    See the Kit »

  • Image
    QXDx BCR-ABL %IS Kit (IVD/CE-IVD)
    2017

    Bio-Rad Unveils QXDx BCR-ABL %IS Kit (IVD/CE-IVD)
    Intended to monitor patients with Chronic Myeloid Leukemia (CML), this FDA-cleared ddPCR kit reports in both International Scale (%IS) and Molecular Response (MR).
    See the Kit »

  • Image
    ddPCR Genome Edit Detection Assays
    2017

    Bio-Rad Releases Paper on ddPCR Genome Edit Detection Assays
    Assays offer a fast, precise, simple, and cost-effective method for detection of genome editing events.
    Read the Bulletin »

  • Image
    QXDx AutoDG ddPCR System
    2019

    The QXDx AutoDG ddPCR System is Shown for the First Time
    Designed for high-throughput processing of samples in a 96-well format, this system simplifies the ddPCR workflow and eliminates user-to-user variability in IVD applications.
    See the System »

  • Image
    Bio-Rad Logo
    2019

    Bio-Rad Releases First FDA-Cleared Digital PCR System & Test for Monitoring Chronic Myeloid Leukemia Treatment Response
    The QXDx AutoDG ddPCR System and QXDx BCR-ABL %IS Kit represent the first-ever digital PCR solution that can monitor and directly quantitate the molecular response of patients with chronic myeloid leukemia under tyrosine kinase inhibitor therapy.
    Read the Press Release »

  • Image
    QX ONE Droplet Digital PCR (ddPCR) System
    2020

    QX ONE™ Droplet Digital™ PCR (ddPCR™) System Release
    A high throughput, multiplexed and integrated Droplet Digital PCR Sytem.
    See the System »

  • Image
    Droplet Digital PCR SARS-CoV-2 Test Kit
    2020

    Bio-Rad Receives FDA Emergency Use Authorization for Droplet Digital PCR SARS-CoV-2 Test Kit
    The SARS-CoV-2 Droplet Digital PCR (ddPCR) test runs on Bio-Rad's QX200 and QXDx ddPCR Systems.
    Read the Press Release »

  • Image
    Vericheck ddPCR Mycoplasma Detection Kit
    2021

    Vericheck ddPCR Mycoplasma Detection Kit Release
    The probe-based Vericheck ddPCR Mycoplasma detection Kit brings confidence in testing for mycoplasma contamination. The specificity and reproducibility of this kit allows for a precise measurement of 112 Mycoplasma species using Droplet Digital PCR.
    See the Kit »

  • Image
    QX600 Droplet Digital PCR System
    2022

    Launch of the QX600™ Droplet Digital™ PCR System
    The QX600 System provides advanced, 6-color multiplexing to maximize the number of biomarkers interrogated per well, allowing the preservation of precious samples and reducing lab burden.
    See the System »

 
 
+ -
 
Image
Talk with a Specialist

Empower Your Research with ddPCR Technology

Our commitment to advancing the field of molecular diagnostics is reflected in the accuracy, sensitivity, and reliability of our ddPCR solutions, making Bio-Rad the trusted choice for researchers worldwide. Join the growing community of scientists who rely on Bio-Rad's ddPCR technology for their groundbreaking discoveries.

Contact a Sales Specialist