PX1™ PCR Plate Sealer

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PX1 PCR Plate Sealer - Amplification | PCR

Overview

PX1™ Heat Sealing Performance

 

Integrating the PX1 PCR Plate Sealer into the Thermal Cycling Workflow Helps Generate Highly Reproducible and Uniform PCR Data

Real-time PCR experiments using the PX1 PCR plate sealer in the workflow demonstrate highly uniform amplification curves. A, a SYBR® Green uniformity assay examined amplification uniformity across an entire 96-well plate sealed using the PX1 PCR plate sealer. The extremely low quantification cycle (Cq) standard deviation of all 96 wells (0.09 cycles) demonstrates exceptional experimental uniformity. B, the SYBR® Green uniformity melt curve analysis demonstrates a single, consistent melt peak across all wells, which indicates product specificity is maintained in plates sealed with the PX1 PCR plate sealer. RFU, relative fluorescence units.

The optically clear heat seal prevents sample evaporation that can lead to large Cq standard deviations. Five-color multiplex linearity test demonstrates highly reliable qPCR results. A–E, fluorescence data from a series of tenfold dilutions of plasmid DNA (108–102 copies) amplified using reporter dyes to monitor five targets. The standard curves of five multiplexed genes have an R2 >0.990. F, standard curves generated from data in A–E. Reaction efficiencies are between 90 and 105%. Cq, quantification cycle; RFU, relative fluorescence units.

Intuitive User Interface
A
  B

The intuitive touch screen makes it easy to program the PX1™ PCR plate sealer. A, high-resolution touch screen presents a button-driven graphical interface to help personalize heat sealing conditions and easily view instrument status; B, the customizable sealing function saves up to 12 unique sealing protocols, eliminating the need to recall optimized sealing conditions.

Heat Sealing Films and Foils
  Optically Clear
Heat Seal
Permanent Clear
Heat Seal
Pierceable Foil
Heat Seal
Peelable Foil
Heat Seal
Catalog number 181-4030 181-4035 181-4040 181-4045
Applications – Real-time PCR
– PCR
– Water bath cycling
– PCR
– QX100™ Droplet Digital™
  PCR system
– PCR
– Sample storage
– PCR
Maximum temperature 110°C 110°C 110°C 110°C
Minimum temperature –80°C –80°C –20°C –200°C
Peelable seal +   + +
Pierceable seal     +  
DNase and RNase free + + + +
Sealing compatibility PP, COC, PE,* PS PP PP, PS* PP, COC*
COC, cyclic olefin copolymer; PE, polyethylene; PP, polypropylene; PS, polystyrene.
* Limited and variable compatibility based on plate composition.

Amplification Reagents and Plastics Brochure
The Amplification Reagents and Plastics Brochure includes information about

 

  • Products for gene expression analysis


    RNA Isolation


    Reverse Transcription Reagents


    Real-Time qPCR Reagents


    PCR Plastic Consumables

  • Product performance data

  • Quick and easy product selection tables

  • Ordering information

 

Download your free copy of the Amplification Reagents and Plastics Brochure.

PX1™ PCR Plate Sealer FAQs

Which heat seal is best for my PCR application?
Use the Heat Sealing Films and Foils table to determine the seal that best suits your experimental needs.

 

How do I determine the best sealing time and temperature?
Optimal sealing parameters vary according to the heat films and microplates used. We recommend sealing an empty plate using the guidelines found in Suggested Heat Sealing Parameters. After sealing, examine the entire plate for effective sealing and optimize sealing conditions if necessary.

 

Some of my wells are not fully sealed. What should I do?
Sealing temperature and/or sealing time may be increased to help improve sealing performance. Use an empty plate to optimize seal conditions. See the instruction manual for additional details about seal optimization.

 

When should I use the sealing frame?
The sealing frame helps secure the heat seal in place and prevents the film from moving during sealing. The sealing frame must be used for the permanent clear heat seal and the peelable foil heat seal.

 

What happens if I accidently place a seal upside down when sealing?
The PX1 heated platen was designed with an easy to clean, nonstick surface. See the PX1™ PCR Plate Sealer Instruction Manual for directions on cleaning the heated platen. Ensure the PX1 sealer cools for at least 1 hour before cleaning.

 

Is it safe to leave the heat sealer on when unattended?
Yes. The PX1 PCR plate sealer was designed for reliability and safety. The instrument has an adjustable auto-off feature that automatically shuts the heater off when not in use for a designated amount of time. Simply touch the screen and the instrument will automatically resume heating.

 

Can I reseal the same plate twice?
A plate sealed with a peelable film may be resealed after peeling an existing film. However, resealing a plate for subsequent use in real-time PCR is not recommended. A plate sealed with a pierceable foil heat seal may also be resealed by sealing another pierceable foil heat seal directly on top. Note: only a single seal can be used in the QX100™ Droplet Digital™ PCR system workflow. Sealing parameters may need to be adjusted.

 

The current platen temperature is higher than the required sealing temperature. How can I reduce the time needed to reach sealing temperature?
The heated platen cools passively. Opening the PX1 PCR plate sealer drawer can help accelerate the cooling process.

 

Can I use traditional adhesive or pressure-sensitive seals with the PX1 sealer?
No. Our heat sealing films feature a special hot melt adhesive that effectively seals microplates when used with the PX1 sealer. The temperature and pressure delivered by the PX1 will not successfully seal a microplate when traditional adhesive and pressure-sensitive seals are used.

The PX1 PCR plate sealer provides consistent and uniform sealing across an entire microplate. This semiautomated heat sealer is ready to seal in less than 3 minutes. The PX1 sealer removes human variability from plate sealing and minimizes sample evaporation from the PCR workflow. This versatile instrument is compatible with a variety of heat sealing films and foils and a wide range of PCR plates.

The PX1 PCR plate sealer features an intuitive touch-screen user interface. The thermal sealing process is simplified by allowing sealing temperature and time to be modified with the touch of a button.

Key Features and Benefits

  • Fast startup time — avoid delaying an experiment while the sealer warms up
  • Extremely intuitive — save time programming the instrument
  • Small footprint — accommodates crowded laboratory benches
  • Quickly access sealing protocols — save time by easily using stored protocols

Heat up time, min <3
Display, cm (in) 10.9 (4.3) color touch screen
Temperature range, °C 100–190, off
Time range, sec 0.5–10.0
Dimensions (W x D x H), cm (in) 19.2 x 24.8 x 21.9 (7.6 x 9.8 x 8.6)
Weight, kg (lb) 7.3 (16)
Power, watts 840
Voltage 100–240 VAC, 50–60 Hz
PX1™ PCR Plate Sealer

181-4000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

List Price:   $3,495.00
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Sealing Frame

181-4080
Sealing frame, helps hold heat sealing films and foils in position, for use with PX1™ PCR plate sealer

List Price:   $62.00
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Plate Support Block

181-4085
Plate support block, holds 96- and 384-well PCR plates in position, for use with PX1™ PCR plate sealer

List Price:   $261.00
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Number Description Options
6257 PX1 PCR Plate Sealer and Heat Sealing Consumables Brochure, Rev A Click to download
10023997 Instruction Manual, PX1 PCR Plate Sealer, Rev A Click to download
10025819 Optically Clear Heat Seal Product Insert, Rev A Click to download
10025820 Permanent Clear Heat Seal Product Insert, Rev A Click to download
10025821 Pierceable Foil Heat Seal Product Insert, Rev A Click to download
10025822 Peelable Foil Heat Seal Product Insert, Rev A Click to download