ddPCR Multiplex Mutation Screening Kits

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ddPCR Multiplex Mutation Screening Kits

Overview

QX200™ Droplet Digital™ PCR System Brochure

Droplet Digital PCR (ddPCR™) is Bio-Rad's unique digital PCR technology.

  • Unrivaled precision provides absolute quantification of target DNA or RNA molecules without the use of standard curves
  • Addresses the lack of scalable and practical technologies for digital PCR implementation

The QX200 ddPCR system makes this powerful technology accessible to you for unveiling previously unattainable
research levels.

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QX200™ Droplet Digital™ (ddPCR™) System Data
Detection of BRAF V600E Mutation

High sensitivity of the QX200 system allows quantification of BRAF V600E mutation in the presence of wild-type DNA using PrimePCR ddPCR assays. A, 2-D fluorescence amplitude plot generated by QuantaSoft™ software shows triplicate wells of a mixed mutant:wild-type sample. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for wild-type DNA only, the blue cluster represents the droplets that are positive for mutant DNA only, and the orange cluster represents the droplets that are positive for both mutant and wild-type DNA. B, 2-D fluorescence amplitude plot shows three replicates of a wild-type-only sample. C, fractional abundance plot shows the percentage frequency (orange markers) of the mutant DNA in a wild-type DNA background. The blue markers indicate the concentration of mutant DNA (copies/µl) and the green markers indicate the concentration of wild-type DNA (copies/µl) in each of three replicate samples. All error bars generated by QuantaSoft software represent the 95% confidence interval.



Detection of Herpes Simplex Virus and β2 Microglobulin

ddPCR enables precise and reproducible detection of herpes simplex virus 1 (HSV-1) and ß2 microglobulin (B2M) targets in a duplex assay. A, 2-D fluorescence amplitude plot shows three merged replicate samples of 10,000 copies/well of HSV-1 duplexed with 20,000 copies/well of Β2M. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for HSV-1 only, the blue cluster represents the droplets that are positive for B2M only, and the orange cluster represents the droplets that are positive for both HSV-1 and B2M targets. B, concentration plot shows merged triplicate wells across an HSV-1 sample dilution series from 0 to 40,000 copies in a constant background of 20,000 copies (66 ng human genomic DNA) of B2M. The blue markers indicate HSV-1 copies/µl and the green markers indicate B2M copies/µl. All error bars generated by QuantaSoft software represent the 95% confidence interval.



Detection of Copy Number for
Myelocytomatosis Oncogene and β-Globin

The QX200 system provides superior resolution with copy number (CN) calls from CN1 to CN13. A, 2-D fluorescence amplitude plot shows duplicate wells of a CN6 sample duplexed with myelocytomatosis oncogene (MYC) and β-globin (HBB) assays. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for MYC only, the blue cluster represents the droplets that are positive for HBB only, and the orange cluster represents the droplets that are positive for both MYC and HBB targets. B, copy number plot shows samples ranging from CN1 to CN13 with precise duplicate well values. All error bars generated by QuantaSoft software represent the 95% confidence interval. NTC, no template control.



Validation for Next-Generation Sequencing

ddPCR provides nearly 1,000-fold greater sensitivity and an amplification bias–free approach compared to RNA-Seq for validation. A, concentration plot shows a twofold dilution of cDNA generated using Bio-Rad’s iScript™ advanced cDNA synthesis kit for RT-qPCR. Two independent measurement sets were made: one at a high concentration range and one at a lower range, with four points overlapping. B, table shows comparative results between ddPCR and MiSeq. ddPCR detected thousands of copies per well with low-abundance transcripts (TBP and GUSB genes) using an input of 100 ng total RNA, which demonstrates enhanced sensitivity. The MiSeq sequencer detected only single-digit reads per kilobase per million reads (RPKM) for these transcripts. ddPCR, Droplet Digital PCR.



Gene Expression Analysis of Stem Cell Markers

High sensitivity and precision of the QX200 system allow reliable detection of small fold changes with rare transcripts. The TGFβ signaling pathway plays a role in ovarian stem cell regulation, and the addition of TGFβ will increase the number of stem cells in the population, increase the expression of putative stem cell markers, and decrease terminal differentiation markers like E-cadherin (ECAD). Data show expression levels of cKIT, CD133, OCT3/OCT4, and ECAD genes across ten samples with and without TGFβ treatment, normalized to PPIA in this study. Reproducible detection of cDNA copies as low as 0.5–1 target/µl in a 20 µl reaction was achieved. S, sample.


Digital PCR using QX200™ and AutoDG™ ddPCR Systems
Success Stories: Articles and Videos

Success Stories: Articles

Genomic Variation Analysis: Single Nucleotide Polymorphism: Validation of Allele-Specific Expression Predicted by RNA-Seq in Human Brain Specimens
In the context of human brain development, the QX100™ Droplet Digital™ PCR System yields unambiguous results for genotype identification of low-expression genes and provides absolute quantification of allele-specific expression (ASE) without the use of a standard.
Download the article.

Genomic Variation Analysis: Copy Number Variation: DUF1220 Copy Number Variation across Healthy and Diseased Populations
Studying copy number variation (CNV) with the QX100 Droplet Digital PCR System allows the robust identification of genetic factors such as DUF1220 in neurogenetic diseases between individuals with greater accuracy and precision, while effectively validating array CGH data.
Download the article.

Genomic Variation Analysis: Single Nucleotide Polymorphism: Detection of Single Nucleotide Variation Frequencies by Droplet Digital™ PCR
The QX100 Droplet Digital PCR System validated DNA sequencing results of induced pluripotent stem cells (iPSCs). In addition to confirming the high frequency of single nucleotide variations (SNVs), a de novo type of SNV was identified.
Download the article.

Cancer Mutation Analysis: How Droplet Digital PCR Has Revolutionized Noninvasive Plasma-Based Detection of Mutations
Performing Droplet Digital PCR with the QX100 Instrument effectively detected and accurately quantified the EGFR T790M marker in targeted therapies with limited cancer tumor samples.
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Viral Quantification: Adeno-Associated Virus Vector Genome Titer Assay
Determining the adeno-associated virus (AAV) vector genome (vg) titer assay is critical in gene therapy applications. The improved sensitivity observed with Droplet Digital PCR (ddPCR™) technology makes it highly suitable for titration of clinical-grade AAV vector preparations.
Download the article.

Gene Expression: Digital Quantification of Potential Therapeutic Target RNAs
Measuring the exact number of long noncoding RNAs (lncRNAs) in subcellular compartments with Droplet Digital PCR (ddPCR) provides insights into their role and mechanism of action.
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Third Generation PCR: A Novel Way to Accurately Measure Copy Number Variations in Human Genome
The McCarroll Lab uses Droplet Digital technology to study the distribution and molecular properties of the genome and its influence on gene expression, as well as the genetics underlying schizophrenia and bipolar disorder.
Download the article.

Success Stories: Videos

Droplet Digital™ PCR opens new perspectives in HIV research. Sangamo BioSciences, Inc. is developing HIV therapeutics toward a functional cure using the company’s proprietary zinc finger DNA-binding protein technology.
Download the article.


Professor Alec Morley, a pioneer in digital PCR, and his colleagues in Adelaide have developed tests for detecting acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) using the unrivaled sensitivity of the QX200 Droplet Digital PCR System.



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Droplet Digital PCR Publications

Browse the growing list of cited peer-reviewed journal articles in Droplet Digital PCR research featuring Bio-Rad products.

Browse Publications

Droplet Digital PCR (ddPCR) Multiplex Mutation Screening Kits are designed for rapid screening of several key cancer mutations in a single reaction with high precision and sensitivity. These kits are ideal for use with formalin-fixed, paraffin-embedded (FFPE) samples, liquid biopsy, fresh/frozen tissue, and samples with low yield or inhibitory substances. The kits contain an optimized, validated ddPCR Mutation Screening Multiplex Assay and ddPCR Supermix for Probes (No dUTP).

Key Benefits

  • Screen multiple mutations in a single well
  • Detect with high sensitivity (limit of detection ≤0.5%)
  • Process numerous samples in a rapid, cost-effective manner
  • Work with low amounts of input DNA without preamplification
  • Use with Bio-Rad’s QX100, QX200, or QX200 AutoDG Systems

Mutations Detected by ddPCR Multiplex Mutation Screening Kits

Catalog# ddPCR Multiplex Mutation Screening Kit* Mutations Detected
12001037 BRAF V600 V600E, V600K, V600R
1863506 KRAS G12/G13 G12A, G12C, G12D, G12R, G12S, G12V, G13D
12001626 KRAS Q61 Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R
12001094 NRAS G12 G12A, G12C, G12D, G12R, G12S, G12V
12001627 NRAS G12/G13 G12A, G12C, G12D, G12S, G12V, G13D, G13R, G13V
12001006 NRAS Q61 Q61H (183A>C), Q61H (183A>T), Q61K, Q61L, Q61R
12002392 EGFR exon19 deletions >15 EGFR exon19 deletions

*Assays for each of the mutations detected by these kits can also be ordered individually. Each kit contains 20x ddPCR Mutation Screening Multiplex Assay (1 x 200 μl) and 2x ddPCR Supermix for Probes (No dUTP) (2 x 1 ml).

Key Actionable Mutations in Various Cancers

Gene Lung Cancer Colorectal Cancer Ovarian Cancer
Thyroid Cancer Melanoma GIST Glioma Pancreatic Cancer
BRAF
KRAS
NRAS
EGFR

GIST, gastrointestinal stromal tumor.

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ddPCR™ KRAS G12/G13 Screening Kit

1863506
200 x 20 µl reactions, includes 20x KRAS G12/G13 assay and ddPCR Supermix for Probes (No dUTP) for screening 7 KRAS G12/G13 mutations using the QX200™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

1863506 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml 1863506 /default/main/bio-rad/WORKAREA/default 10/09/14 05:50 PM en_US juso9kmt 2017-11-14 07:07:31 true true internet/product_SKU juso9kmt templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml LSR 2019-05-04 16:19:21 /default/main/bio-rad/STAGING N /default/main/bio-rad en 10/09/24 05:52 PM 1555973502864 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:19:21 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml 1863506-ddpcr-kras-g12-g13-screening-kit 1863506 ddPCR KRAS Screening Multiplex Kit ddPCR&trade; <em>KRAS G12/G13</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>KRAS G12/G13</em> assay and ddPCR Supermix for Probes (No dUTP) for screening 7 <em>KRAS G12/G13</em> mutations using the QX200&trade; or QX200&trade; AutoDG&trade; Droplet Digital&trade; PCR Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ddpcr-kras-screening-multiplex-kit-186-3506-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ddpcr-kras-screening-multiplex-kit-186-3506.jpg ddPCR&trade; KRAS Screening Multiplex Kit preview ,VN Karen Moss ddPCR&trade; <em>KRAS G12/G13</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x KRAS screening multiplex assay and ddPCR Supermix for Probes (no dUTP), for screening seven KRAS mutations using the QX200&trade; and QX200&trade; AutoDG&trade; QX200&trade; ddPCR&trade; PCR Systems 1863506, ddpcr, kras, gene, mutation, multiplex, screening, assay, supermix, probes, qx200, autodg, droplet, digital, pcr s 10/09/14 05:50 PM 10/09/24 05:52 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <p>Use the ddPCR <em>KRAS G12/G13</em> Screening Kit to screen your samples for the following seven <em>KRAS </em>mutations in a single well:</p> <span class="hidden US-exclude CA-exclude AT-exclude BE-exclude CZ-exclude DK-exclude FI-exclude FR-exclude DE-exclude HU-exclude IT-exclude LU-exclude NO-exclude PL-exclude PT-exclude ES-exclude NL-exclude SE-exclude CH-exclude UK-exclude AU-exclude NZ-exclude SG-exclude CN-exclude TW-exclude HK-exclude MO-exclude TH-exclude ZA-exclude IL-exclude KR-exclude"> <ul> <li>G12A </li> <li>G12C </li> <li>G12D </li> <li>G12R </li> <li>G12S </li> <li>G12V </li> <li>G13D</li> </ul> </span> <div class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IT-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only IL-only KR-only"> <table class="pd_table pd_gridlines" style="width: 70%;" border="0"> <tbody> </tbody> <colgroup> <col width="95"></col> <col width="50"></col> </colgroup> <tbody> <tr> <td class="pd_centeredcell"><strong>Mutation</strong></td> <td class="pd_centeredcell"><strong>Individual Assay ID</strong><br /> <span class="small">(Use links to order assays individually)</span></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G12A</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510586">dHsaMDV2510586</a></td> </tr> <tr> <td class="pd_centeredcell">G12C</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510584">dHsaMDV2510584</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G12D</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510596">dHsaMDV2510596</a></td> </tr> <tr> <td class="pd_centeredcell" height="22">G12R</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510590">dHsaMDV2510590</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G12S</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510588">dHsaMDV2510588</a></td> </tr> <tr> <td class="pd_centeredcell">G12V</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510592">dHsaMDV2510592</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G13D</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510598">dHsaMDV2510598</a></td> </tr> </tbody> </table> </div> <p>This kit is recommended for use with fragmented samples such as FFPE tissue, cell-free DNA, and blood spots.</p> <p>Note: for research use only.</p> <h2 class="descHeader">Kit Components</h2> <ul> <li>20x ddPCR <em>KRAS G12/G13</em> Screening Multiplex Assay &mdash; 1 x 200 &micro;l</li> <li>ddPCR Supermix for Probes (No dUTP) &mdash; 2 x 1 ml</li> </ul> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li><strong>Superior performance</strong> &mdash; allows quantification and screening for multiple <em>KRAS</em> mutations in a single well</li> <li><strong>High sensitivity</strong> &mdash; provides sensitive and precise detection down to 0.2% in a single well</li> <li><strong>Robust workflow</strong> &mdash; allows screening of multiple samples in a rapid and cost-effective manner</li> </ul> <h2 class="descHeader">Additional Equipment</h2> <p>This kit is for use with one or more of the following instruments and accessories:</p> <ul> <li><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=186-4001">QX200 Droplet Digital PCR System</a></li> <li><a href="/en-us/product/qx200-autodg-droplet-digital-pcr-system">QX200 AutoDG Droplet Digital PCR System</a></li> <li><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=181-4000">PX1&trade; PCR Plate Sealer</a></li> <li><a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=185-1197">C1000 Touch&trade; Thermal Cycler with 96 Deep&ndash;Well Reaction Module</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about the features of other <a href="/en-us/product/digital-pcr/digital-pcr-assays">Digital PCR Assays</a> for copy number determination and mutation detection.</p> <h2 class="descHeader">Documents</h2> <ul> <li><a href="/webroot/web/pdf/lsr/literature/10048179.pdf" target="_blank">ddPCR <em>KRAS G12/G13</em> Screening Kit Product Insert</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6407.pdf" target="_blank">Droplet Digital PCR Applications Guide</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6628.pdf" target="_blank">Rare Mutation Detection Best Practices Guidelines</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ddpcr-kras-screening-multiplex-kit-186-3506-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ddpcr-kras-screening-multiplex-kit-186-3506-desc.html Storage at –20°C 2199 Guaranteed up to 18 months in a constant temperature freezer 1 0 1863500 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3500_1412901996.xml 1863503 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3503_1412902192.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1863506&CartQuote=QT&quantity= 1352 false true false $2,320.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1863506&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1863506&quantity= 1863506
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ddPCR™ KRAS Q61 Screening Kit

12001626
200 x 20 µl reactions, includes 20x KRAS Q61 screening assay and ddPCR Supermix for Probes (No dUTP) for screening 5 KRAS Q61 mutations using the QX200™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

12001626 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001626_1449774548.xml 12001626 /default/main/bio-rad/WORKAREA/default 12/10/15 10:54 AM en_US juso9kn5 2017-11-14 07:07:28 true true internet/product_SKU juso9kn5 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001626_1449774548.xml LSR 2019-05-05 00:54:37 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/10/25 11:07 AM 1555973498995 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-05 00:54:37 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001626_1449774548.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001626_1449774548.xml 12001626-ddpcr-kras-q61-screening-kit 12001626 ddPCR KRAS Q61 Screening Kit ddPCR&trade; <em>KRAS Q61</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>KRAS Q61</em> screening assay and ddPCR Supermix for Probes (No dUTP) for screening 5 <em>KRAS Q61</em> mutations using the QX200&trade; or QX200&trade; AutoDG&trade; Droplet Digital&trade; PCR Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ddpcr-kras-q61-screening-kit-12001626-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ddpcr-kras-q61-screening-kit-12001626.jpg ddPCR&trade; <em>KRAS Q61</em> Screening Kit preview ,VN Karen Moss ddPCR <em>KRAS Q61</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>KRAS</em> Q61 screening assay and ddPCR Supermix for Probes (No dUTP) for screening 5 <em>KRAS</em> mutations using the QX200&trade; or QX200&trade; AutoDG&trade; Droplet Digital&trade; PCR Systems 12/10/15 10:54 AM 12/10/25 11:07 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <p>Use the ddPCR <em>KRAS Q61</em> Screening Kit to screen your samples for the following five <em>KRAS</em> mutations in a single well:</p> <span class="hidden US-exclude CA-exclude AT-exclude BE-exclude CZ-exclude DK-exclude FI-exclude FR-exclude DE-exclude HU-exclude IT-exclude LU-exclude NO-exclude PL-exclude PT-exclude ES-exclude NL-exclude SE-exclude CH-exclude UK-exclude AU-exclude NZ-exclude SG-exclude CN-exclude TW-exclude HK-exclude MO-exclude TH-exclude ZA-exclude IL-exclude KR-exclude"> <ul> <li>Q61K</li> <li>Q61L</li> <li>Q61R</li> <li>Q61H 183A&gt;T</li> <li>Q61H 183A&gt;C</li> </ul> </span> <div class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IT-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only IL-only KR-only"> <table class="pd_table pd_gridlines" style="width: 70%;" border="0"> <tbody> </tbody> <colgroup> <col width="95"></col> <col width="50"></col> </colgroup> <tbody> <tr> <td class="pd_centeredcell"><strong>Mutation</strong></td> <td class="pd_centeredcell"><strong>Individual Assay ID</strong><br /> <span class="small">(Use links to order assays individually)</span></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">Q61K</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDS2511862">dHsaMDS2511862</a></td> </tr> <tr> <td class="pd_centeredcell">Q61L</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010101">dHsaMDV2010101</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">Q61R</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010135">dHsaMDV2010135</a></td> </tr> <tr> <td class="pd_centeredcell">Q61H 183A&gt;T</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010131">dHsaMDV2010131</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">Q61H 183A&gt;C</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010133">dHsaMDV2010133</a></td> </tr> </tbody> </table> </div> <p>This kit is recommended for use with fragmented samples such as FFPE tissue, cell-free DNA, and blood spots.</p> <p>Note: for research use only.</p> <h2 class="descHeader">Kit Components</h2> <ul> <li>20x ddPCR <em>KRAS Q61</em> Screening Assay &mdash; 1 x 200 &micro;l</li> <li>ddPCR Supermix for Probes (No dUTP) &mdash; 2 x 1 ml (<a href="/en-us/sku/1863023-ddpcr-supermix-for-probes-no-dutp">1863023</a>)</li> </ul> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li><strong>Superior performance</strong> &mdash; allows quantification and screening for multiple <em>KRAS</em> mutations in a single well</li> <li><strong>High sensitivity</strong> &mdash; provides sensitive and precise detection down to 0.5% in a single well</li> <li><strong>Robust workflow</strong> &mdash; allows screening of multiple samples in a rapid and cost-effective manner</li> </ul> <h2 class="descHeader">Additional Equipment</h2> <p>This kit is for use with one or more of the following instruments and accessories:</p> <ul> <li><a href="/en-us/sku/1864001-qx200-droplet-digital-pcr-system">QX200 Droplet Digital PCR System</a></li> <li><a href="/en-us/product/qx200-autodg-droplet-digital-pcr-system ">QX200 AutoDG Droplet Digital PCR System</a></li> <li><a href="/en-us/sku/1814000-px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer</a></li> <li><a href="/en-us/sku/1851197-c1000-touch-thermal-cycler-with-96-ndash-deep-well-reaction-module">C1000 Touch&trade; Thermal Cycler with 96 Deep&ndash;Well Reaction Module </a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about the features of other <a href="/en-us/product/digital-pcr/digital-pcr-assays">Digital PCR Assays</a> for copy number determination and mutation detection.</p> <h2 class="descHeader">Documents</h2> <ul> <li><a href="/webroot/web/pdf/lsr/literature/10000049691.pdf" target="_blank">ddPCR <em>KRAS Q61</em> Screening Kit Product Insert</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6407.pdf" target="_blank">Droplet Digital PCR Applications Guide</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6628.pdf" target="_blank">Rare Mutation Detection Best Practices Guidelines</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ddpcr-kras-q61-screening-kit-120001626-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ddpcr-kras-q61-screening-kit-120001626-desc.html Storage at –20°C 2199 Guaranteed up to 18 months in a constant temperature freezer 1 0 1863506 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml 12001006 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001006_1449780127.xml 12001037 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001037_1449780230.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001626&CartQuote=QT&quantity= 1352 false true false $2,320.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001626&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001626&quantity= 12001626
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ddPCR™ NRAS G12 Screening Kit

12001094
200 x 20 µl reactions, includes 20x NRAS G12 screening assay and ddPCR Supermix for Probes (No dUTP) for screening 6 NRAS G12 mutations using the QX200™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

12001094 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001094_1449777968.xml 12001094 /default/main/bio-rad/WORKAREA/default 12/10/15 11:55 AM en_US juso9kl5 2017-11-14 07:07:28 true true internet/product_SKU juso9kl5 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001094_1449777968.xml LSR 2019-05-05 00:54:28 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/10/25 12:03 AM 1555973498953 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-05 00:54:28 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001094_1449777968.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001094_1449777968.xml 12001094-ddpcr-nras-g12-screening-kit 12001094 ddPCR NRAS G12 Screening Kit ddPCR&trade; <em>NRAS G12</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>NRAS G12</em> screening assay and ddPCR Supermix for Probes (No dUTP) for screening 6 <em>NRAS G12</em> mutations using the QX200&trade; or QX200&trade; AutoDG&trade; Droplet Digital&trade; PCR Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ddpcr-nras-g12-screening-kit-12001094-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ddpcr-nras-g12-screening-kit-12001094.jpg ddPCR&trade; <em>NRAS G12</em> Screening Kit preview ,VN Karen Moss ddPCR <em>NRAS G12</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>NRAS G12</em> screening assay and ddPCR Supermix for Probes (No dUTP) for screening 6 <em>NRAS</em> mutations using the QX200&trade;/QX100&trade; or AutoDG&trade; Droplet Digital&trade; Systems 12/10/15 11:55 AM 12/10/25 12:03 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <p>Use the ddPCR <em>NRAS G12</em> Screening Kit to screen your samples for the following six <em>NRAS</em> mutations in a single well:</p> <span class="hidden US-exclude CA-exclude AT-exclude BE-exclude CZ-exclude DK-exclude FI-exclude FR-exclude DE-exclude HU-exclude IT-exclude LU-exclude NO-exclude PL-exclude PT-exclude ES-exclude NL-exclude SE-exclude CH-exclude UK-exclude AU-exclude NZ-exclude SG-exclude CN-exclude TW-exclude HK-exclude MO-exclude TH-exclude ZA-exclude IL-exclude KR-exclude"> <ul> <li>G12A <span class="hidden US-only CA-only AT-only BE-only DK-only FI-only FR-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only KR-only">(can be ordered individually: <a href="/digital-assays/#/assay-detail/dHsaMDS42165742">dHsaMDS42165742</a>)</span></li> <li>G12C <span class="hidden US-only CA-only AT-only BE-only DK-only FI-only FR-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only KR-only">(can be ordered individually: <a href="/digital-assays/#/assay-detail/dHsaMDV2510530">dHsaMDV2510530</a>)</span></li> <li>G12D <span class="hidden US-only CA-only AT-only BE-only DK-only FI-only FR-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only KR-only">(can be ordered individually: <a href="/digital-assays/#/assay-detail/dHsaMDV2010095">dHsaMDV2010095</a>)</span></li> <li>G12R <span class="hidden US-only CA-only AT-only BE-only DK-only FI-only FR-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only KR-only">(can be ordered individually: <a href="/digital-assays/#/assay-detail/dHsaMDV2510560">dHsaMDV2510560</a>)</span></li> <li>G12S <span class="hidden US-only CA-only AT-only BE-only DK-only FI-only FR-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only KR-only">(can be ordered individually: <a href="/digital-assays/#/assay-detail/dHsaMDV2010093">dHsaMDV2010093</a>)</span></li> <li>G12V <span class="hidden US-only CA-only AT-only BE-only DK-only FI-only FR-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only KR-only"> (can be ordered individually: <a href="/digital-assays/#/assay-detail/dHsaMDV2510528">dHsaMDV2510528</a>)</span></li> </ul> </span> <div class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IT-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only IL-only KR-only"> <table class="pd_table pd_gridlines" style="width: 70%;" border="0"> <tbody> </tbody> <colgroup> <col width="95"></col> <col width="50"></col> </colgroup> <tbody> <tr> <td class="pd_centeredcell"><strong>Mutation</strong></td> <td class="pd_centeredcell"><strong>Individual Assay ID</strong><br /> <span class="small">(Use links to order assays individually)</span></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G12A</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDS42165742">dHsaMDS42165742</a></td> </tr> <tr> <td class="pd_centeredcell">G12C</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510530">dHsaMDV2510530</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G12D</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010095">dHsaMDV2010095</a></td> </tr> <tr> <td class="pd_centeredcell" height="22">G12R</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510560">dHsaMDV2510560</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G12S</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010093">dHsaMDV2010093</a></td> </tr> <tr> <td class="pd_centeredcell">G12V</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510528">dHsaMDV2510528</a></td> </tr> </tbody> </table> </div> <p>This kit is recommended for use with fragmented samples such as FFPE tissue, cell-free DNA, and blood spots.</p> <p>Note: for research use only.</p> <h2 class="descHeader">Kit Components</h2> <ul> <li>20x ddPCR <em>NRAS G12</em> Screening Assay &mdash; 1 x 200 &micro;l</li> <li>ddPCR Supermix for Probes (no dUTP) &mdash; 2 x 1 ml (<a href="/en-us/sku/1863023-ddpcr-supermix-for-probes-no-dutp">1863023</a>)</li> </ul> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li><strong>Superior performance</strong> &mdash; allows quantification and screening for multiple <em>NRAS</em> mutations in a single well</li> <li><strong>High sensitivity</strong> &mdash; provides sensitive and precise detection down to 0.5% in a single well</li> <li><strong>Robust workflow</strong> &mdash; allows screening of multiple samples in a rapid and cost-effective manner</li> </ul> <h2 class="descHeader">Additional Equipment</h2> <p>This kit is for use with one or more of the following instruments and accessories:</p> <ul> <li><a href="/en-us/sku/1864001-qx200-droplet-digital-pcr-system">QX200 Droplet Digital PCR System</a></li> <li><a href="/en-us/product/qx200-autodg-droplet-digital-pcr-system">QX200 AutoDG Droplet Digital PCR System</a></li> <li><a href="/en-us/sku/1814000-px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer</a></li> <li><a href="/en-us/sku/1851197-c1000-touch-thermal-cycler-with-96-ndash-deep-well-reaction-module">C1000 Touch&trade; Thermal Cycler with 96 Deep&ndash;Well Reaction Module </a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about the features of other <a href="http://www.bio-rad.com/en-us/product/digital-pcr/digital-pcr-assays">Digital PCR Assays</a> for copy number determination and mutation detection.</p> <h2 class="descHeader">Documents</h2> <ul> <li><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10000049688.pdf" target="_blank">ddPCR <em>NRAS G12</em> Screening Kit Product Insert</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6407.pdf" target="_blank">Droplet Digital PCR Applications Guide</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6628.pdf" target="_blank">Rare Mutation Detection Best Practices Guidelines</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ddpcr-nras-g12-screening-kit-12001094-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ddpcr-nras-g12-screening-kit-12001094-desc.html Storage at –20°C 2199 Guaranteed up to 18 months in a constant temperature freezer 1 0 12001627 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001627_1449779815.xml 12001006 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001006_1449780127.xml 1863506 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001094&CartQuote=QT&quantity= 1352 false true false $2,320.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001094&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001094&quantity= 12001094
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ddPCR™ NRAS G12/G13 Screening Kit

12001627
200 x 20 µl reactions, includes 20x NRAS G12/G13 screening assay and ddPCR Supermix for Probes (No dUTP) for screening 8 NRAS G12/G13 mutations using the QX200™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

12001627 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001627_1449779815.xml 12001627 /default/main/bio-rad/WORKAREA/default 12/10/15 12:34 PM en_US juso9kn2 2017-11-14 07:07:28 true true internet/product_SKU juso9kn2 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001627_1449779815.xml LSR 2019-05-05 00:54:24 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/10/25 12:36 AM 1555973499037 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-05 00:54:24 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001627_1449779815.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001627_1449779815.xml 12001627-ddpcr-nras-g12-g13-screening-kit 12001627 ddPCR NRAS G12/G13 Screening Kit ddPCR&trade; <em>NRAS G12/G13</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>NRAS G12/G13</em> screening assay and ddPCR Supermix for Probes (No dUTP) for screening 8 <em>NRAS G12/G13</em> mutations using the QX200&trade; or QX200&trade; AutoDG&trade; Droplet Digital&trade; PCR Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ddpcr-nras-g12-g13-screening-kit-12001627-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ddpcr-nras-g12-g13-screening-kit-12001627.jpg ddPCR&trade; <em>NRAS G12/G13</em> Screening Kit preview ,VN Karen Moss ddPCR <em>NRAS G12/G13</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>NRAS G12/G13</em> screening assay and ddPCR Supermix for Probes (No dUTP) for screening 8 <em>NRAS</em> mutations using the QX200&trade;/QX100&trade; or AutoDG&trade; Droplet Digital&trade; Systems 12/10/15 12:34 PM 12/10/25 12:36 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <p>Use the ddPCR <em>NRAS G12/G13</em> Screening Kit to screen your samples for the following eight <em>NRAS</em> mutations in a single well:</p> <span class="hidden US-exclude CA-exclude AT-exclude BE-exclude CZ-exclude DK-exclude FI-exclude FR-exclude DE-exclude HU-exclude IT-exclude LU-exclude NO-exclude PL-exclude PT-exclude ES-exclude NL-exclude SE-exclude CH-exclude UK-exclude AU-exclude NZ-exclude SG-exclude CN-exclude TW-exclude HK-exclude MO-exclude TH-exclude ZA-exclude IL-exclude KR-exclude"> <ul> <li>G12A</li> <li>G12C</li> <li>G12D</li> <li>G12S</li> <li>G12V</li> <li>G13D</li> <li>G13R</li> <li>G13V</li> </ul> </span> <div class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IT-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only IL-only KR-only"> <table class="pd_table pd_gridlines" style="width: 70%;" border="0"> <tbody> </tbody> <colgroup> <col width="95"></col> <col width="50"></col> </colgroup> <tbody> <tr> <td class="pd_centeredcell"><strong>Mutation</strong></td> <td class="pd_centeredcell"><strong>Individual Assay ID</strong><br /> <span class="small">(Use links to order assays individually)</span></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G12A</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDS42165742">dHsaMDS42165742</a></td> </tr> <tr> <td class="pd_centeredcell">G12C</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510530">dHsaMDV2510530</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G12D</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010095">dHsaMDV2010095</a></td> </tr> <tr> <td class="pd_centeredcell" height="22">G12S</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010093">dHsaMDV2010093</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G12V</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510528">dHsaMDV2510528</a></td> </tr> <tr> <td class="pd_centeredcell">G13D</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510526">dHsaMDV2510526</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">G13R</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510534">dHsaMDV2510534</a></td> </tr> <tr> <td class="pd_centeredcell">G13V</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510524">dHsaMDV2510524</a></td> </tr> </tbody> </table> </div> <p>This kit is recommended for use with fragmented samples such as FFPE tissue, cell-free DNA, and blood spots.</p> <p>Note: for research use only.</p> <h2 class="descHeader">Kit Components</h2> <ul> <li>20x ddPCR <em>NRAS G12/G13</em> Screening Assay &mdash; 1 x 200 &micro;l</li> <li>ddPCR Supermix for Probes (no dUTP) &mdash; 2 x 1 ml (<a href="/en-us/sku/1863023-ddpcr-supermix-for-probes-no-dutp">1863023</a>)</li> </ul> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li><strong>Superior performance</strong> &mdash; allows quantification and screening for multiple <em>NRAS</em> mutations in a single well</li> <li><strong>High sensitivity</strong> &mdash; provides sensitive and precise detection down to 0.5% in a single well</li> <li><strong>Robust workflow</strong> &mdash; allows screening of multiple samples in a rapid and cost-effective manner</li> </ul> <h2 class="descHeader">Additional Equipment</h2> <p>This kit is for use with one or more of the following instruments and accessories:</p> <ul> <li><a href="/en-us/sku/1864001-qx200-droplet-digital-pcr-system">QX200 Droplet Digital PCR System</a></li> <li><a href="/en-us/product/qx200-autodg-droplet-digital-pcr-system">QX200 AutoDG Droplet Digital PCR System</a></li> <li><a href="/en-us/sku/1814000-px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer</a></li> <li><a href="/en-us/sku/1851197-c1000-touch-thermal-cycler-with-96-ndash-deep-well-reaction-module">C1000 Touch&trade; Thermal Cycler with 96 Deep&ndash;Well Reaction Module </a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about the features of other <a href="http://www.bio-rad.com/en-us/product/digital-pcr/digital-pcr-assays">Digital PCR Assays</a> for copy number determination and mutation detection.</p> <h2 class="descHeader">Documents</h2> <ul> <li><a href="/webroot/web/pdf/lsr/literature/10000049690.pdf" target="_blank">ddPCR <em>NRAS G12/G13</em> Screening Kit Product Insert</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6407.pdf" target="_blank">Droplet Digital PCR Applications Guide</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6628.pdf" target="_blank">Rare Mutation Detection Best Practices Guidelines</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ddpcr-nras-g12-g13-screening-kit-12001627-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ddpcr-nras-g12-g13-screening-kit-12001627-desc.html Storage at –20°C 2199 Guaranteed up to 18 months in a constant temperature freezer 1 0 12001094 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001094_1449777968.xml 12001006 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001006_1449780127.xml 12001037 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001037_1449780230.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001627&CartQuote=QT&quantity= 1352 false true false $2,320.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001627&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001627&quantity= 12001627
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ddPCR™ NRAS Q61 Screening Kit

12001006
200 x 20 µl reactions, includes 20x NRAS Q61 screening assay and ddPCR Supermix for Probes (No dUTP) for screening 5 NRAS Q61 mutations using the QX200™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

12001006 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001006_1449780127.xml 12001006 /default/main/bio-rad/WORKAREA/default 12/10/15 12:40 PM en_US juso9kmz 2017-11-14 07:07:28 true true internet/product_SKU juso9kmz templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001006_1449780127.xml LSR 2019-05-05 00:54:24 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/10/25 12:41 AM 1555973498785 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-05 00:54:24 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001006_1449780127.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001006_1449780127.xml 12001006-ddpcr-nras-q61-screening-kit 12001006 ddPCR NRAS Q61 Screening Kit ddPCR&trade; <em>NRAS Q61</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>NRAS Q61</em> screening assay and ddPCR Supermix for Probes (No dUTP) for screening 5 <em>NRAS Q61</em> mutations using the QX200&trade; or QX200&trade; AutoDG&trade; Droplet Digital&trade; PCR Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ddpcr-nras-q61-screening-kit-12001006-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ddpcr-nras-q61-screening-kit-12001006.jpg ddPCR&trade; <em>NRAS Q61</em> Screening Kit preview ,VN Karen Moss ddPCR <em>NRAS Q61</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>NRAS Q61</em> screening assay and ddPCR Supermix for Probes (No dUTP) for screening 5 <em>NRAS</em> mutations using the QX200&trade;/QX100&trade; or AutoDG&trade; Droplet Digital&trade; Systems 12/10/15 12:40 PM 12/10/25 12:41 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <p>Use the ddPCR <em>NRAS Q61</em> Screening Kit to screen your samples for the following five <em>NRAS</em> mutations in a single well:</p> <span class="hidden US-exclude CA-exclude AT-exclude BE-exclude CZ-exclude DK-exclude FI-exclude FR-exclude DE-exclude HU-exclude IT-exclude LU-exclude NO-exclude PL-exclude PT-exclude ES-exclude NL-exclude SE-exclude CH-exclude UK-exclude AU-exclude NZ-exclude SG-exclude CN-exclude TW-exclude HK-exclude MO-exclude TH-exclude ZA-exclude IL-exclude KR-exclude"> <ul> <li>Q61K</li> <li>Q61L</li> <li>Q61R</li> <li>Q61H</li> <li>Q61H 183A&gt;C</li> </ul> </span> <div class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IT-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only IL-only KR-only"> <table class="pd_table pd_gridlines" style="width: 70%;" border="0"> <tbody> </tbody> <colgroup> <col width="95"></col> <col width="50"></col> </colgroup> <tbody> <tr> <td class="pd_centeredcell"><strong>Mutation</strong></td> <td class="pd_centeredcell"><strong>Individual Assay ID</strong><br /> <span class="small">(Use links to order assays individually)</span></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">Q61K</td> <td class="pd_centeredcell"><span class="hidden US-only CA-only AT-only BE-only DK-only FI-only FR-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only KR-only"><a href="/digital-assays/#/assay-detail/dHsaMDV2010067">dHsaMDV2010067</a></span></td> </tr> <tr> <td class="pd_centeredcell">Q61L</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010069">dHsaMDV2010069</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">Q61R</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010071">dHsaMDV2010071</a></td> </tr> <tr> <td class="pd_centeredcell" height="22">Q61H 183A&gt;T</td> <td class="pd_centeredcell"><span class="hidden US-only CA-only AT-only BE-only DK-only FI-only FR-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only KR-only"><a href="/digital-assays/#/assay-detail/dHsaMDV2010065">dHsaMDV2010065</a></span></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">Q61H 183A&gt;C</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2510578">dHsaMDV2510578</a></td> </tr> </tbody> </table> </div> <p>This kit is recommended for use with fragmented samples such as FFPE tissue, cell-free DNA, and blood spots.</p> <p>Note: for research use only.</p> <h2 class="descHeader">Kit Components</h2> <ul> <li>20x ddPCR <em>NRAS Q61</em> Screening Assay &mdash; 1 x 200 &micro;l</li> <li>ddPCR Supermix for Probes (no dUTP) &mdash; 2 x 1 ml (<a href="/en-us/sku/1863023-ddpcr-supermix-for-probes-no-dutp">1863023</a>)</li> </ul> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li><strong>Superior performance</strong> &mdash; allows quantification and screening for multiple <em>NRAS</em> mutations in a single well</li> <li><strong>High sensitivity</strong> &mdash; provides sensitive and precise detection down to 0.5% in a single well</li> <li><strong>Robust workflow</strong> &mdash; allows screening of multiple samples in a rapid and cost-effective manner</li> </ul> <h2 class="descHeader">Additional Equipment</h2> <p>This kit is for use with one or more of the following instruments and accessories:</p> <ul> <li><a href="/en-us/sku/1864001-qx200-droplet-digital-pcr-system">QX200 Droplet Digital PCR System</a></li> <li><a href="/en-us/product/qx200-autodg-droplet-digital-pcr-system">QX200 AutoDG Droplet Digital PCR System</a></li> <li><a href="/en-us/sku/1814000-px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer</a></li> <li><a href="/en-us/sku/1851197-c1000-touch-thermal-cycler-with-96-ndash-deep-well-reaction-module">C1000 Touch&trade; Thermal Cycler with 96 Deep&ndash;Well Reaction Module </a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about the features of other <a href="http://www.bio-rad.com/en-us/product/digital-pcr/digital-pcr-assays">Digital PCR Assays</a> for copy number determination and mutation detection.</p> <h2 class="descHeader">Documents</h2> <ul> <li><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10000049686.pdf" target="_blank">ddPCR <em>NRAS Q61</em> Screening Kit Product Insert</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6407.pdf" target="_blank">Droplet Digital PCR Applications Guide</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6628.pdf" target="_blank">Rare Mutation Detection Best Practices Guidelines</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ddpcr-nras-q61-screening-kit-12001006-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ddpcr-nras-q61-screening-kit-12001006-desc.html Storage at –20°C 2199 Guaranteed up to 18 months in a constant temperature freezer 1 0 12001094 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001094_1449777968.xml 12001627 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001627_1449779815.xml 12001037 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001037_1449780230.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001006&CartQuote=QT&quantity= 1352 false true false $2,320.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001006&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001006&quantity= 12001006
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ddPCR™ BRAF V600 Screening Kit

12001037
200 x 20 µl reactions, includes 20x BRAF V600 screening assay and ddPCR Supermix for Probes (No dUTP) for screening 3 BRAF V600 mutations using the QX200™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

12001037 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001037_1449780230.xml 12001037 /default/main/bio-rad/WORKAREA/default 12/10/15 12:42 PM en_US juso9kkh 2017-11-14 07:07:28 true true internet/product_SKU juso9kkh templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001037_1449780230.xml LSR 2019-05-05 00:54:26 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/10/25 12:43 AM 1555973498827 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-05 00:54:26 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001037_1449780230.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001037_1449780230.xml 12001037-ddpcr-braf-v600-screening-kit 12001037 ddPCR BRAF V600 Screening Kit ddPCR&trade; <em>BRAF V600</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>BRAF V600</em> screening assay and ddPCR Supermix for Probes (No dUTP) for screening 3 <em>BRAF V600</em> mutations using the QX200&trade; or QX200&trade; AutoDG&trade; Droplet Digital&trade; PCR Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ddpcr-braf-v600-screening-kit-12001037-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ddpcr-braf-v600-screening-kit-12001037.jpg ddPCR&trade; <em>BRAF V600</em> Screening Kit preview ,VN Karen Moss ddPCR <em>BRAF V600</em> Screening Kit 200 x 20 &micro;l reactions, includes 20x <em>BRAF V600</em> screening assay and ddPCR Supermix for Probes (No dUTP) for screening 3 <em>BRAF</em> mutations using the QX200&trade;/QX100&trade; or AutoDG&trade; Droplet Digital&trade; Systems 12/10/15 12:42 PM 12/10/25 12:43 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <p>Use the ddPCR <em>BRAF V600</em> Screening Kit to screen your samples for the following three <em>BRAF </em>mutations in a single well:</p> <span class="hidden US-exclude CA-exclude AT-exclude BE-exclude CZ-exclude DK-exclude FI-exclude FR-exclude DE-exclude HU-exclude IT-exclude LU-exclude NO-exclude PL-exclude PT-exclude ES-exclude NL-exclude SE-exclude CH-exclude UK-exclude AU-exclude NZ-exclude SG-exclude CN-exclude TW-exclude HK-exclude MO-exclude TH-exclude ZA-exclude IL-exclude KR-exclude"> <ul> <li>V600E</li> <li>V600K</li> <li>V600R</li> </ul> </span> <div class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IT-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only IL-only KR-only"> <table class="pd_table pd_gridlines" style="width: 70%;" border="0"> <tbody> </tbody> <colgroup> <col width="95"></col> <col width="50"></col> </colgroup> <tbody> <tr> <td class="pd_centeredcell"><strong>Mutation</strong></td> <td class="pd_centeredcell"><strong>Individual Assay ID</strong><br /> <span class="small">(Use links to order assays individually)</span></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">V600E</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010027">dHsaMDV2010027</a></td> </tr> <tr> <td class="pd_centeredcell">V600K</td> <td class="pd_centeredcell"><a href="/digital-assays/#/assay-detail/dHsaMDV2010035">dHsaMDV2010035</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">V600R</td> <td class="pd_centeredcell"><span class="hidden US-only CA-only AT-only BE-only DK-only FI-only FR-only DE-only IT-only LU-only NO-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only PL-only HU-only CZ-only IL-only KR-only"><a href="/digital-assays/#/assay-detail/dHsaMDV2010037">dHsaMDV2010037</a></span></td> </tr> </tbody> </table> </div> <p>This kit is recommended for use with fragmented samples such as FFPE tissue, cell-free DNA, and blood spots.</p> <p>Note: for research use only.</p> <h2 class="descHeader">Kit Components</h2> <ul> <li>20x ddPCR <em>BRAF V600</em> Screening Assay &mdash; 1 x 200 &micro;l</li> <li>ddPCR Supermix for Probes (no dUTP) &mdash; 2 x 1 ml (<a href="/en-us/sku/1863023-ddpcr-supermix-for-probes-no-dutp">1863023</a>)</li> </ul> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li><strong>Superior performance</strong> &mdash; allows quantification and screening for multiple <em>BRAF</em> mutations in a single well</li> <li><strong>High sensitivity</strong> &mdash; provides sensitive and precise detection down to 0.5% in a single well</li> <li><strong>Robust workflow</strong> &mdash; allows screening of multiple samples in a rapid and cost-effective manner</li> </ul> <h2 class="descHeader">Additional Equipment</h2> <p>This kit is for use with one or more of the following instruments and accessories:</p> <ul> <li><a href="/en-us/sku/1864001-qx200-droplet-digital-pcr-system">QX200 Droplet Digital PCR System</a></li> <li><a href="/en-us/product/qx200-autodg-droplet-digital-pcr-system">QX200 AutoDG Droplet Digital PCR System</a></li> <li><a href="/en-us/sku/1814000-px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer</a></li> <li><a href="/en-us/sku/1851197-c1000-touch-thermal-cycler-with-96-ndash-deep-well-reaction-module">C1000 Touch&trade; Thermal Cycler with 96 Deep&ndash;Well Reaction Module </a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about the features of other <a href="http://www.bio-rad.com/en-us/product/digital-pcr/digital-pcr-assays">Digital PCR Assays</a> for copy number determination and mutation detection.</p> <h2 class="descHeader">Documents</h2> <ul> <li><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10000049687.pdf" target="_blank">ddPCR <em>BRAF V600 </em>Screening Kit Product Insert</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6407.pdf" target="_blank">Droplet Digital PCR Applications Guide</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6628.pdf" target="_blank">Rare Mutation Detection Best Practices Guidelines</a></li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global ddpcr-braf-v600-screening-kit-12001037-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/ddpcr-braf-v600-screening-kit-12001037-desc.html Storage at –20°C 2199 Guaranteed up to 18 months in a constant temperature freezer 1 0 1863506 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml 12001627 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001627_1449779815.xml 12001626 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001626_1449774548.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001037&CartQuote=QT&quantity= 1352 false true false $2,320.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001037&AddToDefaultWishList=true&quantity= true false true 41106300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12001037&quantity= 12001037
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ddPCR™ EGFR Exon 19 Deletions Screening Kit

12002392
200 x 20 µl reactions, includes 20x EGFR Exon 19 Deletions screening assay and ddPCR Supermix for Probes (no dUTP) for screening 15 EGFR Exon 19 Deletions using the QX100™, QX200™ or AutoDG™ Droplet Digital™ Systems

12002392 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12002392_1471982952.xml 12002392 /default/main/bio-rad/WORKAREA/default 08/23/16 01:04 PM en_US juso9kkz 2017-11-14 07:07:28 true true internet/product_SKU juso9kkz templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12002392_1471982952.xml LSR 2019-05-05 00:54:15 /default/main/bio-rad/STAGING N /default/main/bio-rad en 08/23/26 01:05 PM 1555973499248 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-05 00:54:15 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12002392_1471982952.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12002392_1471982952.xml 12002392-ddpcr-egfr-exon-19-deletions-screening-kit 12002392 ddPCR? EGFR Exon 19 Deletions Screening Kit ddPCR™ <em>EGFR</em> Exon 19 Deletions Screening Kit 200 x 20 µl reactions, includes 20x <em>EGFR</em> Exon 19 Deletions screening assay and ddPCR Supermix for Probes (no dUTP) for screening 15 <em>EGFR</em> Exon 19 Deletions using the QX100™, QX200™ or AutoDG™ Droplet Digital™ Systems 200 x 20 µl reactions, includes 20x <em>EGFR</em> Exon 19 Deletions screening assay and ddPCR Supermix for Probes (no dUTP) for screening 15 <em>EGFR</em> Exon 19 Deletions using the QX100™, QX200™ or AutoDG™ Droplet Digital™ Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ddpcr-egfr-exon-19-deletions-screening-kit-12002392-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ddpcr-egfr-exon-19-deletions-screening-kit-12002392.jpg preview ,VN Jessica Low ddPCR™ EGFR Exon 19 Deletions Screening Kit ddPCR™ EGFR Exon 19 Deletions Screening Kit 12002392, ddpcr, droplet digital pcr, digital pcr, mutation, screening, assay, egfr, exon 19, deletion, deletions 08/23/16 01:04 PM 08/23/26 01:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <p>Use the ddPCR <em>EGFR</em> Exon 19 Deletions Screening Kit to screen your samples for the following 15 <em>EGFR</em> Exon 19 Deletions mutations in a single well:</p> <span class="hidden US-exclude CA-exclude AT-exclude BE-exclude CZ-exclude DK-exclude FI-exclude FR-exclude DE-exclude HU-exclude IT-exclude LU-exclude NO-exclude PL-exclude PT-exclude ES-exclude NL-exclude SE-exclude CH-exclude UK-exclude AU-exclude NZ-exclude SG-exclude CN-exclude TW-exclude HK-exclude MO-exclude TH-exclude ZA-exclude IL-exclude KR-exclude"> <ul> <li>2235_2252&gt;AAT</li> <li>2235_2249del15</li> <li>2236_2250del15</li> <li>2238_2252&gt;GCA</li> <li>2238_2255del18</li> <li>2239_2253&gt;CAA</li> <li>2239_2251&gt;C</li> <li>2239_2258&gt;CA</li> <li>2239_2252&gt;CA</li> <li>2239_2256del18</li> <li>2239_2248TTAAGAGAAG&gt;C</li> <li>2239_2253del15</li> <li>2239_2247delTTAAGAGAA</li> <li>2240_2254del15</li> <li>2240_2257del18</li> </ul> </span> <div class="hidden US-only CA-only AT-only BE-only CZ-only DK-only FI-only FR-only DE-only HU-only IT-only LU-only NO-only PL-only PT-only ES-only NL-only SE-only CH-only UK-only AU-only NZ-only SG-only CN-only TW-only HK-only MO-only TH-only ZA-only IL-only KR-only"> <table class="pd_table pd_gridlines" style="width: 80%;" border="0"> <tbody> </tbody> <colgroup> <col width="5"></col> <col width="2"></col> </colgroup> <tbody> <tr> <td class="pd_centeredcell"><strong>Mutation</strong></td> <td class="pd_centeredcell"><strong>Individual Assay ID</strong><br /> <span class="small">(Use links to order assays individually)</span></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">2235_2252&gt;AAT</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDS391737271">dHsaMDS391737271</a></td> </tr> <tr> <td class="pd_centeredcell">2235_2249del15</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDV2010039">dHsaMDV2010039</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">2236_2250del15</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDS542127747">dHsaMDS542127747</a></td> </tr> <tr> <td class="pd_centeredcell" height="22">2238_2252&gt;GCA</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDS435546404">dHsaMDS435546404</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">2238_2255del18</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDS529140399">dHsaMDS529140399</a></td> </tr> <tr> <td class="pd_centeredcell">2239_2253&gt;CAA</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDS703176693">dHsaMDS703176693</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">2239_2251&gt;C</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDV2516890">dHsaMDV2516890</a></td> </tr> <tr> <td class="pd_centeredcell">2239_2258&gt;CA</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDS196056967">dHsaMDS196056967</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">2239_2252&gt;CA</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDS651224081">dHsaMDS651224081</a></td> </tr> <tr> <td class="pd_centeredcell">2239_2256del18</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDV2516758">dHsaMDV2516758</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">2239_2248TTAAGAGAAG&gt;C</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDV2516748">dHsaMDV2516748</a></td> </tr> <tr> <td class="pd_centeredcell">2239_2253del15</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDV2516752">dHsaMDV2516752</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">2239_2247delTTAAGAGAA</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDS88236242">dHsaMDS88236242</a></td> </tr> <tr> <td class="pd_centeredcell">2240_2254del15</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDS778667043">dHsaMDS778667043</a></td> </tr> <tr class="pd_colorbackground"> <td class="pd_centeredcell">2240_2257del18</td> <td class="pd_centeredcell"><a href="https://www.bio-rad.com/digital-assays/#/assay-detail/dHsaMDV2510546">dHsaMDV2510546</a></td> </tr> </tbody> </table> </div> <p>This kit is recommended for use with fragmented samples such as FFPE tissue, cell-free DNA, and blood spots.</p> <p>Note: for research use only.</p> <h2 class="descHeader">Kit Components</h2> <ul> <li>20x ddPCR EGFR Exon 19 Deletions Screening Assay &mdash; 1 x 200 &micro;l</li> <li>ddPCR Supermix for Probes (no dUTP) &mdash; 2 x 1 ml (<a href="/en-us/sku/1863023-ddpcr-supermix-for-probes-no-dutp">1863023</a>)</li> </ul> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li><strong>Superior performance </strong>&mdash; allows quantification and screening of 15<em> EGFR </em>Exon 19 Deletions in a single well</li> <li><strong>High sensitivity</strong> &mdash; provides sensitive and precise detection down to 0.5%</li> <li><strong>Robust workflow </strong>&mdash; allows screening of multiple samples in a rapid and cost-effective manner</li> </ul> <h2 class="descHeader">Additional Equipment</h2> <p>This kit is for use with one or more of the following instruments and accessories:</p> <ul> <li><a href="/en-us/sku/1864001-qx200-droplet-digital-pcr-system">QX200 Droplet Digital PCR System</a>&nbsp; </li> <li><a href="/en-us/product/qx200-autodg-droplet-digital-pcr-system">QX200 AutoDG Droplet Digital PCR System </a></li> <li><a href="/en-us/sku/1814000-px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer </a></li> <li><a href="/en-us/sku/1851197-c1000-touch-thermal-cycler-with-96-ndash-deep-well-reaction-module">C1000 Touch&trade; Thermal Cycler with 96 Deep&ndash;Well Reaction Module </a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about the features of other <a href="http://www.bio-rad.com/en-us/product/digital-pcr/digital-pcr-assays">Digital PCR Assays</a> for copy number determination and mutation detection.</p> <h2 class="descHeader">Documents</h2> <ul> <li><a href="/webroot/web/pdf/lsr/literature/10000052123.pdf" target="_blank">ddPCR <em>EGFR</em> Exon 19 Deletions Screening Kit Product Insert</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6407.pdf" target="_blank">Droplet Digital PCR Applications Guide</a></li> <li><a href="/webroot/web/pdf/lsr/literature/Bulletin_6628.pdf" target="_blank">Rare Mutation Detection Best Practices Guidelines</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated ddpcr_egfr_exon_19_deletions_screen_kit_12002392.html /webroot/web/html/lsr/products/SKUs/autopopulated/ddpcr_egfr_exon_19_deletions_screen_kit_12002392.html Storage at –20°C 2199 Guaranteed up to 18 months in a constant temperature freezer 1 0 1863506 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml 12001627 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001627_1449779815.xml 12001037 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001037_1449780230.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12002392&CartQuote=QT&quantity= 1352 false true false $2,320.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12002392&AddToDefaultWishList=true&quantity= true false true 12352200 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=12002392&quantity= 12002392
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Number Description Options
10048179 ddPCR™ KRAS Screening Multiplex Kit Product Insert, Rev A Click to download
10000049686 ddPCR™ NRAS Q61 Screening Kit Product Insert, Rev A Click to download
10000049687 ddPCR™ BRAF V600 Screening Kit Product Insert, Rev A Click to download
10000049688 ddPCR™ NRAS G12 Screening Kit Product Insert, Rev A Click to download
10000049690 ddPCR™ NRAS G12/G13 Screening Kit Product Insert, Rev B Click to download
10000049691 ddPCR™ KRAS Q61 Screening Kit Product Insert, Rev A Click to download
10000052123 ddPCR™ EGFR Exon 19 Deletions Screening Kit Product Insert, Ver C Click to download

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