QX200™ AutoDG™ Droplet Digital™ PCR System

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QX200 AutoDG Droplet Digital PCR


The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR™) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical.

The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.

Test Drive the QX200 AutoDG Droplet Digital PCR System

Launch the AutoDG Interactive Web App to explore the setup and loading steps of the Automated Droplet Generator and see how the AutoDG System turns your samples into droplets ready for Droplet Digital PCR.
Launch AutoDG Interactive Web App

Features and Benefits of the QX200 AutoDG Droplet Digital PCR System

Automated Droplet Generator — simplifies the ddPCR workflow and offers flexibility:

  • Generates droplets for 96 ddPCR reactions in under 45 minutes
  • Compatible with both the QX100™ and QX200™ Droplet Digital™ PCR Systems
  • A single AutoDG Instrument can supply 4–5 Droplet Readers continuously
  • Guides setup and loading with large, color touch-screen interface and LED lighting
  • User-definable plate layout with increments of 8 wells provides run-to-run flexibility
  • HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment

Droplet Digital PCR Technology — the most precise and sensitive digital PCR solution for a wide variety of applications, providing:

  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Nanoliter droplet partitioning — reduces bias from amplification efficiency differences and PCR inhibitors
  • Convenient assay design — standard curves are not required

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation — measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution.
  • Pathogen detection — employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring
  • Next generation sequencing — perform absolute quantification of an NGS library without the use of a standard curve
  • Gene expression analysis — achieve reliable and reproducible measurements of small fold changes for low-abundance mRNA and miRNA
  • Environmental monitoring — test a wide variety of environmental samples such as soil and water
  • Food testing — perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods

Required Instruments

The QX200 AutoDG Droplet Digital PCR System requires the use of the C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module and the PX1™ PCR Plate Sealer.

Required Consumables

ddPCR Reagents for EvaGreen or TaqMan Hydrolysis Probe-Based Applications

More Information

Droplet Digital PCR applications guide Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including:
  • Copy number variation analysis
  • Rare mutation and sequence detection
  • Gene expression
  • Next-generation sequencing library analysis

View Performance Data for the QX200 Droplet Digital PCR System.

Watch our Success Stories Videos to learn more about the applications of the Droplet Digital PCR System in diverse research fields.

Browse our Droplet Digital PCR Reference Library with links to scientific journal articles, conference posters, BioRadiations articles, and Bio-Rad technical reports.

Visit our Applications & Technologies pages to learn more about Droplet Digital PCR Technology and the digital PCR workflow.

Starting sample size, µl 20
Automated Droplet Generator capacity 1–96 samples/run
Droplets per 20 µl sample 20,000
QX200™ Droplet Reader capacity 1–96 samples
Sample illumination Light-emitting diodes
Sample detection Multi-pixel photon counter
Detection channels FAM (EvaGreen), HEX (VIC)
Linear dynamic range 5 orders of magnitude
Precision ±10%
Droplets per 96-well plate, million ~1.5
Automated Droplet Generator dimensions (W x D x H) Open: 66 x 56 x 89 cm (26 x 22 x 35 in)
Closed: 66 x 56 x 66 cm (26 x 22 x 26 in)
QX200 Droplet Reader dimensions (W x D x H) 66 x 52 x 29 cm (26 x 20 x 11 in)
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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

QX200™ AutoDG™ Droplet Digital™ PCR System

1864100
AutoDG Droplet Digital PCR System for EvaGreen or probe-based digital PCR applications, includes Automated Droplet Generator, QX200 Droplet Reader, laptop computer, and QuantaSoft™ Software

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Automated Droplet Generator

1864101
AutoDG™ Instrument for automated droplet generation of up to 96 samples, for use with the QX200™/QX100™ Droplet Digital™ PCR Systems

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QX200™ Droplet Reader

1864003
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200™ Droplet Digital™ System

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Number Description Options
6450 Droplet Digital™ PCR Publications List, Rev L Click to download
6561 QX200™ AutoDG™ Droplet Digital™ PCR System Flier, Rev B Click to download [ Add to Cart (Free) ]
10043138 Instruction Manual, Automated Droplet Generator for Droplet Digital™ PCR Systems, Rev B Click to download
10028376 QX200™ ddPCR™ EvaGreen Supermix Product Insert, Rev C Click to download
10048259 ddPCR™ Supermix for Residual DNA Quantification Product Insert, Rev A Click to download
6782 ddPCR™ Multiplex Mutation Screening Kits Flier, Ver B Click to download [ Add to Cart (Free) ]
6721 ddPCR™ Supermix for Residual DNA Quantification Flier, Rev A Click to download
6712 Ultra-Sensitive Quantification of Genome Editing Events Using Droplet Digital™ PCR Application Note, Ver B Click to download