ddPCR Residual DNA Quantification Kits

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ddPCR Residual DNA Quantification Kits

Overview

QX200™ Droplet Digital™ PCR System Brochure

Droplet Digital PCR (ddPCR™) is Bio-Rad's unique digital PCR technology.

  • Unrivaled precision provides absolute quantification of target DNA or RNA molecules without the use of standard curves
  • Addresses the lack of scalable and practical technologies for digital PCR implementation

The QX200 ddPCR system makes this powerful technology accessible to you for unveiling previously unattainable
research levels.

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QX200™ Droplet Digital™ (ddPCR™) System Data
Detection of BRAF V600E Mutation

High sensitivity of the QX200 system allows quantification of BRAF V600E mutation in the presence of wild-type DNA using PrimePCR ddPCR assays. A, 2-D fluorescence amplitude plot generated by QuantaSoft™ software shows triplicate wells of a mixed mutant:wild-type sample. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for wild-type DNA only, the blue cluster represents the droplets that are positive for mutant DNA only, and the orange cluster represents the droplets that are positive for both mutant and wild-type DNA. B, 2-D fluorescence amplitude plot shows three replicates of a wild-type-only sample. C, fractional abundance plot shows the percentage frequency (orange markers) of the mutant DNA in a wild-type DNA background. The blue markers indicate the concentration of mutant DNA (copies/µl) and the green markers indicate the concentration of wild-type DNA (copies/µl) in each of three replicate samples. All error bars generated by QuantaSoft software represent the 95% confidence interval.



Detection of Herpes Simplex Virus and β2 Microglobulin

ddPCR enables precise and reproducible detection of herpes simplex virus 1 (HSV-1) and ß2 microglobulin (B2M) targets in a duplex assay. A, 2-D fluorescence amplitude plot shows three merged replicate samples of 10,000 copies/well of HSV-1 duplexed with 20,000 copies/well of Β2M. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for HSV-1 only, the blue cluster represents the droplets that are positive for B2M only, and the orange cluster represents the droplets that are positive for both HSV-1 and B2M targets. B, concentration plot shows merged triplicate wells across an HSV-1 sample dilution series from 0 to 40,000 copies in a constant background of 20,000 copies (66 ng human genomic DNA) of B2M. The blue markers indicate HSV-1 copies/µl and the green markers indicate B2M copies/µl. All error bars generated by QuantaSoft software represent the 95% confidence interval.



Detection of Copy Number for
Myelocytomatosis Oncogene and β-Globin

The QX200 system provides superior resolution with copy number (CN) calls from CN1 to CN13. A, 2-D fluorescence amplitude plot shows duplicate wells of a CN6 sample duplexed with myelocytomatosis oncogene (MYC) and β-globin (HBB) assays. The black cluster on the plot represents the negative droplets, the green cluster represents the droplets that are positive for MYC only, the blue cluster represents the droplets that are positive for HBB only, and the orange cluster represents the droplets that are positive for both MYC and HBB targets. B, copy number plot shows samples ranging from CN1 to CN13 with precise duplicate well values. All error bars generated by QuantaSoft software represent the 95% confidence interval. NTC, no template control.



Validation for Next-Generation Sequencing

ddPCR provides nearly 1,000-fold greater sensitivity and an amplification bias–free approach compared to RNA-Seq for validation. A, concentration plot shows a twofold dilution of cDNA generated using Bio-Rad’s iScript™ advanced cDNA synthesis kit for RT-qPCR. Two independent measurement sets were made: one at a high concentration range and one at a lower range, with four points overlapping. B, table shows comparative results between ddPCR and MiSeq. ddPCR detected thousands of copies per well with low-abundance transcripts (TBP and GUSB genes) using an input of 100 ng total RNA, which demonstrates enhanced sensitivity. The MiSeq sequencer detected only single-digit reads per kilobase per million reads (RPKM) for these transcripts. ddPCR, Droplet Digital PCR.



Gene Expression Analysis of Stem Cell Markers

High sensitivity and precision of the QX200 system allow reliable detection of small fold changes with rare transcripts. The TGFβ signaling pathway plays a role in ovarian stem cell regulation, and the addition of TGFβ will increase the number of stem cells in the population, increase the expression of putative stem cell markers, and decrease terminal differentiation markers like E-cadherin (ECAD). Data show expression levels of cKIT, CD133, OCT3/OCT4, and ECAD genes across ten samples with and without TGFβ treatment, normalized to PPIA in this study. Reproducible detection of cDNA copies as low as 0.5–1 target/µl in a 20 µl reaction was achieved. S, sample.


Digital PCR using QX200™ and AutoDG™ ddPCR Systems
Success Stories: Articles and Videos

Success Stories: Articles

Genomic Variation Analysis: Single Nucleotide Polymorphism: Validation of Allele-Specific Expression Predicted by RNA-Seq in Human Brain Specimens
In the context of human brain development, the QX100™ Droplet Digital™ PCR System yields unambiguous results for genotype identification of low-expression genes and provides absolute quantification of allele-specific expression (ASE) without the use of a standard.
Download the article.

Genomic Variation Analysis: Copy Number Variation: DUF1220 Copy Number Variation across Healthy and Diseased Populations
Studying copy number variation (CNV) with the QX100 Droplet Digital PCR System allows the robust identification of genetic factors such as DUF1220 in neurogenetic diseases between individuals with greater accuracy and precision, while effectively validating array CGH data.
Download the article.

Genomic Variation Analysis: Single Nucleotide Polymorphism: Detection of Single Nucleotide Variation Frequencies by Droplet Digital™ PCR
The QX100 Droplet Digital PCR System validated DNA sequencing results of induced pluripotent stem cells (iPSCs). In addition to confirming the high frequency of single nucleotide variations (SNVs), a de novo type of SNV was identified.
Download the article.

Cancer Mutation Analysis: How Droplet Digital PCR Has Revolutionized Noninvasive Plasma-Based Detection of Mutations
Performing Droplet Digital PCR with the QX100 Instrument effectively detected and accurately quantified the EGFR T790M marker in targeted therapies with limited cancer tumor samples.
Download the article.

Viral Quantification: Adeno-Associated Virus Vector Genome Titer Assay
Determining the adeno-associated virus (AAV) vector genome (vg) titer assay is critical in gene therapy applications. The improved sensitivity observed with Droplet Digital PCR (ddPCR™) technology makes it highly suitable for titration of clinical-grade AAV vector preparations.
Download the article.

Gene Expression: Digital Quantification of Potential Therapeutic Target RNAs
Measuring the exact number of long noncoding RNAs (lncRNAs) in subcellular compartments with Droplet Digital PCR (ddPCR) provides insights into their role and mechanism of action.
Download the article.

Third Generation PCR: A Novel Way to Accurately Measure Copy Number Variations in Human Genome
The McCarroll Lab uses Droplet Digital technology to study the distribution and molecular properties of the genome and its influence on gene expression, as well as the genetics underlying schizophrenia and bipolar disorder.
Download the article.

Success Stories: Videos

Droplet Digital™ PCR opens new perspectives in HIV research. Sangamo BioSciences, Inc. is developing HIV therapeutics toward a functional cure using the company’s proprietary zinc finger DNA-binding protein technology.
Download the article.


Professor Alec Morley, a pioneer in digital PCR, and his colleagues in Adelaide have developed tests for detecting acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) using the unrivaled sensitivity of the QX200 Droplet Digital PCR System.



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Droplet Digital PCR Publications

Browse the growing list of cited peer-reviewed journal articles in Droplet Digital PCR research featuring Bio-Rad products.

Browse Publications

Residual host cell DNA (HCD) that carries over during the process of manufacturing therapeutic proteins and vaccines poses safety concerns and must not exceed levels established by regulatory agencies such as the U.S. Food and Drug Administration and the World Health Organization. Bio-Rad’s ddPCR Residual DNA Quantification Kits are ideal for highly precise quantification of HCD in complex bioprocess intermediates. The kits contain an optimized ddPCR CHO or E. coli Residual Quantification Assay and ddPCR Supermix for Residual DNA Quantification. Both assay and supermix are guaranteed free of contaminating DNA.

Key Benefits

The Residual DNA Quantification Kits are optimized to enable:

  • Highly precise, femtogram-level quantification of residual CHO or coli DNA
  • Direct quantification without DNA purification steps
  • Compatible with Bio-Rad’s QX100, QX200, or QX200 AutoDG Droplet Digital PCR Systems

Sample Data

Direct Sample Input: Eliminate DNA Extraction Prior to Residual Host Cell DNA Quantification

Many biopharmaceutical products (biologics, vaccines, etc.) are produced in mammalian or bacterial host cells. DNA levels and matrix conditions vary during the purification process, making residual host cell DNA extraction difficult. Inhibitory substances may also be present, making quantification by qPCR difficult and inaccurate. ddPCR eliminates the need for DNA extraction, as partitioning in ddPCR minimizes the effect of inhibitory substances on the PCR reaction, enabling accurate quantification from complex bioprocess intermediates using direct sample input. Proteinase K and DTT can also be used prior to ddPCR to enable direct input
of samples containing up to 5 mg/ml of protein (Figure 1).

Fig. 1. DNA detection in samples with high IgG levels using Proteinase K pretreatment. CHO DNA was spiked into a sample matrix composed of 100 mg/ml IgG, 3% mannitol, 2% sucrose, 10 mM L-arginine, and 0.01% Tween 20. The sample was then treated with PK and diluted to 100, 10, and 5 fg of CHO DNA prior to ddPCR analysis. CHO standards were also included as controls to determine recovery of the test sample. Full recovery of the CHO DNA was obtained at the three concentrations tested. The data show good linearity, sensitivity, and IgG tolerance. The amount of IgG equivalent in the 100 fg CHO DNA sample is 5 mg/ml. CHO DNA standard (); CHO spiked matrix + PK ().

Sensitive and Precise HCD Quantification

ddPCR CHO and E. coli Residual DNA Quantification Kits enable highly sensitive and precise detection and quantification of host cell DNA without a standard curve. The ddPCR CHO Residual DNA Quantification Kit can reliably detect as little as 1 fg of DNA with a limit of quantification (LOQ) of ≤15 fg per 20 µl reaction and a linear range of 3 fg–3 pg. The ddPCR E. coli Residual DNA Quantification Kit can reliably detect as little as 15 fg of DNA with an LOQ of ≤30 fg per 20 µl reaction and a linear range of 30 fg–3 pg (Figure 2).

Free of CHO and E. coli Contaminating DNA

The ddPCR CHO and E. coli Residual DNA Quantification Assays and ddPCR Residual DNA Quantification Supermixes are guaranteed to be free of contaminating CHO and E. coli DNA.


Fig. 2. Titration of E. coli and CHO DNA. A, titration of E. coli DNA from 3 fg to 30 pg; B, titration of CHO DNA from 0.3 fg to 3 pg. Both plots display highly reproducible results between different lots of assays. Lot 1 (); lot 2 (); lot 3 ().

Consistent Performance Even with Highly Fragmented DNA

The ddPCR CHO and E. coli Residual DNA Quantification Assays yield accurate quantification of results, regardless of the size of the DNA molecules. There is less than 20% variation in concentration calls with intact or fragmented DNA.

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ddPCR™ E. coli Residual DNA Quantification Kit

17000032
200 x 20 µl reactions, includes 20x E. coli Residual DNA Quantification Assay and ddPCR Supermix for Residual DNA Quantification. For use with the QX100™, QX200™, or AutoDG™ Droplet Digital™ PCR Systems

17000032 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000032_1456425911.xml 17000032 /default/main/bio-rad/WORKAREA/default 02/25/16 10:10 AM en_US juso9kmd 2017-11-14 07:07:28 true true internet/product_SKU juso9kmd templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000032_1456425911.xml LSR 2019-05-05 00:54:20 /default/main/bio-rad/STAGING N /default/main/bio-rad en 02/25/26 10:42 AM 1555973499838 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-05 00:54:20 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000032_1456425911.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000032_1456425911.xml 17000032-ddpcr-e-coli-residual-dna-quantification-kit 17000032 ddPCR Residual DNA Quant E.coli Kit ddPCR&trade; <i>E. coli</i> Residual DNA Quantification Kit 200 x 20 &micro;l reactions, includes 20x <em>E. coli</em> Residual DNA Quantification Assay and ddPCR Supermix for Residual DNA Quantification. For use with the QX100&trade;, QX200&trade;, or AutoDG&trade; Droplet Digital&trade; PCR Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ddpcr-e-coli-residual-dna-quantification-kit-sku-17000032-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ddpcr-e-coli-residual-dna-quantification-kit-sku-17000032-view.jpg preview ,VN Molly Gregg ddPCRTM E. coli Residual DNA Quantification Kit 200 x 20 µl reactions, includes 20x E.coli Residual DNA Quantification Assay and ddPCR Supermix for Residual DNA Quantification for E.coli residual DNA quantification using the QX100™, QX200™, or AutoDG™ Droplet Digital™ Systems 02/25/16 10:10 AM 02/25/26 10:42 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <p>Use the ddPCR <em>E. coli</em> Residual DNA Quantification Kit to detect femtogram amounts of residual <em>E. coli</em> host cell DNA (HCD) in various types of bioproduction intermediates.</p> <p>To ensure product quality and safety of biopharmaceutical products, the amount of residual host cell DNA must meet guidelines established by regulatory agencies including the World Health Organization, the European Pharmacopeia, and the U.S. Food and Drug Administration.</p> <p>Note: for research use only.</p> <h2 class="descHeader">Kit Components</h2> <ul> <li> 20x ddPCR <em>E. coli</em> Residual DNA Quantification Assay &mdash; 1 x 200 &micro;l</li> <li> ddPCR Supermix for Residual DNA Quantification &mdash; 2 x 1 ml </li> </ul> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li> <strong>Direct sample input </strong>&mdash; eliminates the need for DNA extraction; partitioning minimizes the effects of inhibitors</li> <li> <strong>Highly sensitive, precise detection and quantification of HCD without a standard curve:</strong> <ul> <li>LOD = 15 fg or better</li> <li>LOQ &le;30 fg per 20 &micro;l reaction</li> <li>Range = 30 fg&ndash;30 pg</li> </ul> </li> <li><strong>Minimal method development</strong> &mdash; assay and supermix guaranteed free of detectable levels of CHO and <em>E. coli</em> DNA</li> <li> <strong>Accurate quantification even with highly fragmented DNA</strong> &mdash; kit detects 16s rRNA genes in fragments &ge;200 bp</li> <li> <strong>Fast, simple workflow</strong> &mdash; completed within hours rather than days</li> <li> <strong>High sample throughput</strong> &mdash; allows processing of several hundred samples in a single day</li> </ul> <h2 class="descHeader">Additional Equipment</h2> <p>This kit is for use with one or more of the following instruments and accessories:</p> <ul> <li> <a href="/en-us/sku/1864001-qx200-droplet-digital-pcr-system">QX200 Droplet Digital PCR System</a></li> <li> <a href="/en-us/product/qx200-autodg-droplet-digital-pcr-system">QX200 AutoDG Droplet Digital PCR System</a></li> <li> <a href="/en-us/sku/1814000-px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer</a></li> <li> <a href="/en-us/sku/1851197-c1000-touch-thermal-cycler-with-96-ndash-deep-well-reaction-module">C1000 Touch&trade; Thermal Cycler with 96 Deep&ndash;Well Reaction Module</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about the features of other <a href="/en-us/product/digital-pcr-assays ">Digital PCR Assays</a> for copy number determination and mutation detection.</p> <h2 class="descHeader">Documents</h2> <ul> <li><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10000050187.pdf" target="_blank">ddPCR <em>E. coli</em> Residual DNA Quantification Kit Product Insert</a> </li> </ul> /webroot/web/html/lsr/products/SKUs 1700003.html /webroot/web/html/lsr/products/SKUs/1700003.html Storage at –20°C 2199 Guaranteed up to 18 months in a constant temperature freezer 1 0 17000031 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000031_1456426161.xml 1863506 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml 12001006 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001006_1449780127.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=17000032&CartQuote=QT&quantity= 1352 false true false $3,010.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=17000032&AddToDefaultWishList=true&quantity= true false true 12352200 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=17000032&quantity= 17000032
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ddPCR™ CHO Residual DNA Quantification Kit

17000031
200 x 20 µl reactions, includes 20x CHO Residual DNA Quantification Assay and ddPCR Supermix for Residual DNA Quantification. For use with the QX100™, QX200™, or QX200™ AutoDG™ Droplet Digital™ PCR Systems

17000031 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000031_1456426161.xml 17000031 /default/main/bio-rad/WORKAREA/default 02/25/16 10:09 AM en_US juso9kne 2017-11-14 07:07:28 true true internet/product_SKU juso9kne templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000031_1456426161.xml LSR 2019-05-05 00:54:26 /default/main/bio-rad/STAGING N /default/main/bio-rad en 02/25/26 10:48 AM 1555973499796 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-05 00:54:26 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000031_1456426161.xml templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000031_1456426161.xml 17000031-ddpcr-cho-residual-dna-quantification-kit 17000031 ddPCR Residual DNA Quant CHO Kit ddPCR&trade; CHO Residual DNA Quantification Kit 200 x 20 &micro;l reactions, includes 20x CHO Residual DNA Quantification Assay and ddPCR Supermix for Residual DNA Quantification. For use with the QX100&trade;, QX200&trade;, or QX200&trade; AutoDG&trade; Droplet Digital&trade; PCR Systems /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ddpcr-cho-residual-dna-quantification-kit-sku-17000031-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ddpcr-cho-residual-dna-quantification-kit-sku-17000031-view.jpg preview ,VN Molly Gregg ddPCR CHO Residual DNA Quantification Kit 200 x 20 µl reactions, includes 20x CHO Residual DNA Quantification Assay and ddPCR Supermix for CHO Residual DNA Quantification using the QX100™, QX200™, or QX200™ AutoDG™ Droplet Digital™ Systems 02/25/16 10:09 AM 02/25/26 10:48 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/Droplet-Digital-PCR-System N 0 <p>Use the ddPCR CHO Residual DNA Quantification Kit to detect femtogram amounts of residual CHO host cell DNA (HCD) in various types of bioproduction intermediates.</p> <p>To ensure product quality and safety of biopharmaceutical products, the amount of residual host cell DNA must meet guidelines established by regulatory agencies including the World Health Organization, the European Pharmacopeia, and the U.S. Food and Drug Administration.</p> <p>Note: for research use only.</p> <h2 class="descHeader">Kit Components</h2> <ul> <li> 20x ddPCR CHO Residual DNA Quantification Assay &mdash; 1 x 200 &micro;l</li> <li> ddPCR Supermix for Residual DNA Quantification &mdash; 2 x 1 ml</li> </ul> <h2 class="descHeader">Key Features and Benefits</h2> <ul> <li> <strong>Direct sample input </strong>&mdash; eliminates the need for DNA extraction; partitioning minimizes the effects of inhibitors</li> <li> <strong>Highly sensitive, precise detection and quantification of HCD without a standard curve:</strong> <ul> <li>LOD = 1 fg or better</li> <li>LOQ &le;15 fg per 20 &micro;l reaction </li> <li>Range = 3 fg&ndash;3 pg</li> </ul> </li> <li><strong>Minimal method development</strong> &mdash; assay and supermix guaranteed free of detectable levels of CHO and <em>E. coli</em> DNA</li> <li> <strong>Accurate quantification even with highly fragmented DNA</strong> &mdash; kit detects SINE fragments &ge;200 bp</li> <li> <strong>Fast, simple workflow</strong> &mdash; completed within hours rather than days</li> <li> <strong>High sample throughput</strong> &mdash; allows processing of several hundred samples in a single day</li> </ul> <h2 class="descHeader">Additional Equipment</h2> <p>This kit is for use with one or more of the following instruments and accessories:</p> <ul> <li> <a href="/en-us/sku/1864001-qx200-droplet-digital-pcr-system">QX200 Droplet Digital PCR System</a></li> <li> <a href="/en-us/product/qx200-autodg-droplet-digital-pcr-system">QX200 AutoDG Droplet Digital PCR System</a></li> <li> <a href="/en-us/sku/1814000-px1-pcr-plate-sealer">PX1&trade; PCR Plate Sealer</a></li> <li> <a href="/en-us/sku/1851197-c1000-touch-thermal-cycler-with-96-ndash-deep-well-reaction-module">C1000 Touch&trade; Thermal Cycler with 96 Deep&ndash;Well Reaction Module</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>Learn more about the features of other <a href="/en-us/product/digital-pcr-assays">Digital PCR Assays</a> for copy number determination and mutation detection.</p> <h2 class="descHeader">Documents</h2> <ul> <li><a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10000050186.pdf" target="_blank">ddPCR CHO Residual DNA Quantification Kit Product Insert</a> </li> </ul> /webroot/web/html/lsr/products/SKUs 17000031.html /webroot/web/html/lsr/products/SKUs/17000031.html Storage at –20°C 2199 Guaranteed up to 18 months in a constant temperature freezer 1 0 17000032 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/17000032_1456425911.xml 1863506 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/186-3506_1412902404.xml 12001006 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/Droplet-Digital-PCR-System/12001006_1449780127.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=17000031&CartQuote=QT&quantity= 1352 false true false $3,120.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=17000031&AddToDefaultWishList=true&quantity= true false true 12352200 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=17000031&quantity= 17000031
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Number Description Options
10000050186 ddPCR™ CHO Residual DNA Quantification Kit, Rev A Click to download
10000050187 ddPCR™ E. coli Residual DNA Quantification Kit, Rev A Click to download

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