Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (immunodetection) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.
Related Topics: Protein Blotting Methods, Membranes and Blotting Papers, Transfer Buffers, and Transfer Conditions.
See how you can streamline your protein electrophoresis and western blotting experiments.
Protein detection systems and their sensitivity.
Call us at 1-800-4-BIORAD (1-800-424-6723)
Please reenter your email address in the correct format.
Please enter your email address.
Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile.
To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. You can elect to receive only the types of Bio-Rad communications that are of interest to you..
Copyright © 2016 Bio-Rad Laboratories, Inc. All rights reserved.