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Description
Description
Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. This resin can be used as part of the Protein Expression and Purification Series .
- Chelating ligand is iminodiacetic acid (IDA)
- Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems
- Stable across a wide pH range (1–14)
- Works under native or denaturing conditions
- Can be used for single-step purification from crude samples or contaminant removal
Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.
Related Products
- Centrifugation Purification Module (1665041EDU) used in the third module purification option in Protein Expression and Purification Series Classroom Study Kit
- Prepacked Bio-Scale™ Mini Profinity™ IMAC Cartridges with Ni-charged IDA, 5 x 1 ml (7324610EDU), 1 x 5 ml (7324612EDU), and 5 x 5 ml (7324614EDU)
- Poly-Prep® Chromatography Columns (7311550EDU) hold up to 2ml support
Related Categories
- Protein Expression and Purification Series
- Biotechnology: A Laboratory Skills Course
- Chromatography
- BioLogic™ Low-Pressure Liquid Chromatography Systems
- Fraction Collectors
- Chromatography Media
- Prepacked Columns
Supporting Documents
- Profinity IMAC Resins Instruction Manual
- Profinity IMAC Resin Product Information Sheet
- Profinity IMAC Ni-Charged Resin Flier
- Profinity IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification Tech Note
- Bio-Scale Mini Profinity IMAC Cartridges, 1 and 5 ml Instruction Manual
- Bio-Scale Mini Profinity IMAC Cartridge Product Information Sheet