Aminex Organic Acid and Alcohol Analysis Columns

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Analysis of N-acetylneuramic acid on Aminex HPX-87H column

Analysis of N-acetylneuramic acid in urine samples on theAminex HPX-87H column. A. Standard N-acetylneuraminic acid; B. Urinesample from control subject; C. Urine sample from a patient with sialuria.Reproduced with permission, Mononen, I., J. Chrom., 381, 219 (1986).

Aminex® HPLC Columns Selection Guide

Selection guide for Aminex carbohydrate, organic acid, and alcohol analysis columns.

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Aminex HPLC columns are packed with a polymer-based matrix (polystyrene divinylbenzene). They separate compounds using the ion-moderated partition chromatography technique.

HPLC separations using Aminex columns employ the mechanisms of ion exclusion, ion exchange, ligand exchange, size exclusion, reversed phase, and normal phase partitioning. These multiple modes of interaction offer a unique ability to separate compounds. Several Aminex columns are available for small acidic and polar molecule analysis.

Aminex columns allow the use of simple isocratic methods, eluting with water or dilute acid. There is minimal sample preparation, usually just filtering through a 0.45 μm filter with no derivatization necessary. Aminex media have a high pressure stability, wide pH stability, and high column efficiency and selectivity.

Aminex columns are an industry standard for the analysis of organic acids and alcohols.Bio-Rad offers organic acid columns for simple, yet specific methods of food and beverage analysis. Use our selection guide to choose the column that fits your application needs.

Aminex HPX-87H Column
This hydrogen-form, 300 x 7.8 mm column performs most analyses in about 20 minutes, with sensitivity to the nanogram level. A simple isocratic elution with slightly acidified water is all that it takes to analyze organic acids in most samples, and filtration is the only sample preparation required before injection. Separations can be performed from ambient temperature to 60°C at flow rates of 0.4–1.0 ml/min. When many compounds in a formulation must be analyzed, or when high-resolution separations are required, this is the column of choice.

Fast Acid Analysis Column
This hydrogen-form, 100 x 7.8 mm column is optimized for the analysis of alcohols, glycols, and hydrophobic organic acids. Typically, analyses can be shortened 4-fold over those obtained with research-length columns. Because the fast acid column is shorter, components elute as taller, narrower peaks. Detection limits are improved, and smaller sample loads can be used. This column provides fast separations of specific organic acids in samples where only certain components are of interest. With these columns, analyses can be completed in 3–5 minutes. As with the Aminex HPX-87H column, dilute sulfuric acid is the only eluent used, and filtering is generally the only sample preparation required.

Fermentation Monitoring Column
This hydrogen-form, 150 x 7.8 mm column is optimized to resolve maltotriose, maltose, glucose, and fructose while also resolving acids and alcohols. When analysis of sugars in a fermentation broth is required, this is the column of choice. Dilute sulfuric acid is the only eluent used, and filtering is generally the only sample preparation required.

Aminex HPX-87C Column
This calcium-form, 300 x 7.8 mm column can be fine-tuned for organic acid analysis by using an eluent that contains a mixture of water, calcium sulfate, sulfuric acid, and acetonitrile. It is also suited for analyzing organic acids in combination with carbohydrates.

Aminex HPX-87H Column

125-0140
Pkg of 1, 300 x 7.8 mm, prepacked HPLC carbohydrate analysis column, hydrogen form, 9 µm particle size, 8% cross linkage, pH range 1–3

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Fast Acid Analysis Column

125-0100
Pkg of 1, 100 x 7.8 mm, prepacked HPLC organic acid and alcohol column, hydrogen form, 9 μm particle size, 8% cross linkage, pH range 1–3

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Fermentation Monitoring Column

125-0115
Pkg of 1, 150 x 7.8 mm, prepacked HPLC organic acid and alcohol column, hydrogen form, 9 μm particle size, 8% cross linkage, pH range 1–3

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Organic Acid Analysis Kit

125-0234
Application kit for food analysis, hydrogen form, 9 μm particle size, 8% cross linkage, pH range 1–3, includes guard column

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Number Description Options
6333 Aminex HPLC Columns Selection Guide, Interactive PDF, Rev A Click to download
M1250131 Instruction Manual, Standard Cartridge Holder and Deashing Holder, Rev B Click to download
M1250147 Instruction Manual, Seal Replacement Instructions for Micro-Guard Cartridges, Rev B Click to download
LIT15 Instruction Manual, Micro-Guard Cartridges, Rev D Click to download
1189 Micro-Guard HPLC Guard Column Product Information Sheet, Rev A Click to download
LIT42 Instruction Manual, Aminex Resin-Based Columns, Rev D Click to download [ Add to Cart (Free) ]
1895 Bibliography of Aminex HPLC Column References, Rev A Click to download
1928 Overview and Strategies for Bio-Organic Molecule Purification, Rev B Click to download