Description
Description
Teach PCR, gel electrophoresis, and bioinformatics with this DNA barcoding lab activity in which students determine the species of a fish based on the DNA sequence of its cytochrome c oxidase I (COI) gene. Students first extract DNA from tissue, use PCR to amplify the DNA, and perform agarose gel electrophoresis to verify PCR products. Next, after receiving DNA sequencing results from a third party (sequencing services not included), students then use bioinformatics to genetically identify the species using the sequence obtained. The unique COI sequence produced for a given sample is considered its DNA barcode.
This cost-saving bundle includes agarose and TAE electrophoresis buffer.
With this kit, you can teach your students how to:
- Perform DNA extraction, PCR amplification, and gel electrophoresis up to 16 fish DNA and control samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation)
- Use DNA sequencing and bioinformatics to determine species identity
- Perform investigations in their area to examine biodiversity or see if marketplace substitution is occurring
- Contribute to global initiatives for barcoding life (Barcode of Life Database)
Learn more on the DNA Barcoding Kits product page.
Timeline
Finish all lab activities in three 45-minute class periods.
Product Contents
- DNA Extraction Module: reagents and consumable plastics for DNA extraction (12016408EDU)
- Fish PCR Module: reagents and consumable plastics for PCR amplification of the fish COI gene, control DNA, DNA loading dye and stain, and molecular weight ruler (12016300EDU)
- Certified molecular biology agarose, 25 g (1613100EDU)
- 50x Tris/Acetic Acid/EDTA (TAE), 100 ml (1660742EDU)
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