Bio-Plex Pro Human SARS-CoV-2 Variant Neutralization Antibody 11-Plex Panel #12016897

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Bio-Plex SARS-CoV-2 Variant SKU

Overview

1 x 96-well, includes SARS-CoV-2 wild-type and variant coupled magnetic capture beads, biotinylated detection ACE2 receptor, standard, ready-to-use positive control, assay buffer, wash buffer, serology sample diluent, streptavidin-phycoerythrin (SA-PE), 96-well flat bottom plate, and sealing tape. For the detection of human neutralization antibodies against SARS-CoV-2 RBD, S1, Alpha S1, Beta S1, Gamma RBD, D614G S1, E484K RBD, Epsilon RBD, K417N RBD, Kappa RBD, N501Y RBD antigens.

Safety Data Sheet

Safety Data Sheet

Description

Description

Contact and Expert

Questions? Ask a Bio-Plex Specialist.

Bio-Plex SARS-CoV-2 Neutralization Antibody Assays Webinar

The Bio-Plex Pro Human SARS-CoV-2 Variant Neutralization Antibody 11-Plex Panel quantitatively measure SARS-CoV-2 neutralizing antibodies against SARS-CoV-2 wild-type RBD, Spike 1 (S1), Alpha S1, Beta S1, Gamma RBD, D614G S1, E484K RBD, Epsilon RBD, K417N RBD, Kappa RBD, N501Y RBD antigens. SARS-CoV-2 variant antigens are coated on magnetic beads that bind neutralizing antibodies in competition with a biotinylated detection ACE2 receptor. The highly precise and specific assay panel enables researchers to assess efficacy of COVID-19 vaccines or compare effectiveness of naturally acquired or vaccine-induced antibody response to arising variants of concern. One kit includes all components for a complete assay.

Features and Benefits

  • Comprehensive panel coverage — detects neutralizing antibody response to two wild-type and nine variants of Receptor Binding Domain (RBD) and Spike 1 (S1) protein subunits in a single well
  • Fast protocol — results in less than 3 hours
  • High precision and specificity — for greater confidence in measuring SARS-CoV-2 specific neutralizing antibodies in serum and plasma samples
  • Reproducibility — attain consistent results in your experiments with a robust, magnetic bead-based workflow

Kit Components

  • Magnetic capture beads coupled with two SARS-CoV-2 wild type and nine variants of Receptor Binding Domain (RBD) and Spike 1 (S1) protein subunits:
    • RBD
    • S1
    • Alpha S1
    • Beta S1
    • Gamma RBD
    • D614G S1 
    • E484K RBD 
    • Epsilon RBD
    • K417N RBD
    • Kappa RBD
    • N501Y RBD
  • Biotinylated detection ACE2 receptor
  • Neutralization antibody standard
  • Ready-to-use positive control
  • Assay buffer and wash buffer
  • Serology sample diluent
  • Streptavidin-PE
  • 96-well flat-bottom plate and sealing tape

Individual kit components can also be purchased to create custom assays.

Instrument Compatibility

Compatible with all xMAP Platforms:

*For Research Use Only. Not for use in diagnostic procedures.

Specifications

Specifications

Reactive species
Human
Intra-assay precision, %CV
<10%
Inter-assay precision, %CV
<20%
Clinical specificity
(n = 118 negative samples)
RBD = 100%, S1 = 99%, Alpha S1 = 100%, Beta S1 = 100%, Gamma RBD = 97%, D614G S1 = 100%, E484K RBD = 99%, Epsilon RBD = 100%, K417N RBD = 96%, Kappa RBD = 99%, N501Y RBD = 99%
Clinical sensitivity
(n = 84 SARS-CoV-2 IgG positive samples)
RBD = 98%, S1 = 98%, Alpha S1 = 99%, Beta S1 = 90%, Gamma RBD = 90%, D614G S1 = 99%, E484K RBD = 89%, Epsilon RBD = 95%, K417N RBD = 99% , Kappa RBD = 93%, N501Y RBD = 99%
Compatible sample matrices
Serum, plasma

Documents

Documents

Number Description Options
10000147006
Instruction Manual, Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays
10000147009
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays Quick Guide
3263
Bio-Plex™ Pro Human SARS-CoV-2 Neutralization Antibody Assays Product Information Sheet
10000148774
Bio-Plex Amine Coupling Kit Instruction Manual
3286
Development of New SARS-CoV-2 Variant Neutralization Antibody Assays Using the Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit Application Note