Zeta-Probe Membranes

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Overview

Zeta-Probe membranes are high-tensile-strength cationized nylon membranes that carry a high-density quaternary amine charge. These pliable, flexible membranes are resistant to tearing.
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Description

Description

Effective September 25, 2020 — The Zeta Probe Blotting Membrane release paper, used to wrap membrane rolls or placed in between individual membrane discs, has been changed from blue to brown. The new, brown paper is FDA food contact grade and has BSE/TSE certification. This change should have no impact on the performance nor properties of the products that they are used to package. There have been no changes to the shelf-life, form, fit, claims, release specifications, literature, validation guide, nor the quality certificate.

Zeta-Probe Membranes are high-tensile-strength cationized nylon membranes that carry a high-density quaternary amine charge. These pliable, flexible membranes are resistant to shrinking, tearing, and becoming brittle during transfer, hybridization, or reprobing procedures.

Zeta-Probe Membranes bind nucleic acids independently of buffer pH, so they can be used in traditional Southern blots, rapid alkaline Southern or northern blotting techniques, and electrophoretic transfer of nucleic acids from agarose and polyacrylamide gels.

Zeta-Probe Membranes can be repeatedly hybridized and stripped at 100°C in 0.5% SDS, 0.1x SSC as many as 20 times for DNA (Li et al. 1987) and 6 times for RNA (Gatti et al. 1984). Oligonucleotides as short as 6 bases will bind to the membrane, and oligonucleiotides ≥ 20 bases long will be retained after repeated hybridization and washing.

References

Gatti RA et al. (1984). Multiple uses of Southern blots. Biotechniques 2, 148-155.

Li JK et al. (1987). Rapid alkaline blot-transfer of viral dsRNAs. Anal Biochem 163, 210-218.

Ordering

Ordering

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1620153
Pkg of 15, 9 x 12 cm, high-strength nylon membrane positively charged with quaternary amine groups
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1620154
Pkg of 15, 10 x 15 cm, high-strength nylon membrane positively charged with quaternary amine groups
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1620155
Pkg of 15, 15 x 15 cm, high-strength nylon membrane positively charged with quaternary amine groups
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1620156
Pkg of 15, 15 x 20 cm, high-strength nylon membrane positively charged with quaternary amine groups
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1620157
Pkg of 15, 20 x 20 cm, high-strength nylon membrane positively charged with quaternary amine groups
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1620158
Pkg of 3, 20 x 25 cm, high-strength nylon membrane positively charged with quaternary amine groups
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1620159
Pkg of 1 roll, 30 cm x 3.3 m, high-strength nylon membrane positively charged with quaternary amine groups
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1620165
Pkg of 1 roll, 20 cm x 3.3 m, high-strength nylon membrane positively charged with quaternary amine groups
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1620206
The 7 x 10 cm Zeta-Probe Membrane is now discontinued, and the 10 x 15 cm Zeta-Probe Membrane is recommended as an alternative product.

Kits & Reagents

Kits & Reagents

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(Discontinued) Gel Drying Solution
1610752
1 L, gel drying solution for use with polyacrylamide gels
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1610781
1 L, detergent, for easy pipetting
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(Discontinued) CHAPS
1610460
1 g, CHAPS detergent powder
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1610418
1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution
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1610416
250 ml, 10% (w/v) sodium dodecyl sulfate (SDS) solution
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1610407
500 ml, Triton X-100 detergent
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1610301
100 g, sodium dodecyl sulfate (SDS) powder
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(Discontinued) Cleaning Concentrate
1610722
1 kg, 50x alkaline detergent for cleaning glass plates and other laboratory equipment

Documents

Documents

Number Description Options
1110
Zeta-Probe® Blotting Membranes for Northern, Southern, Alkaline Southern, and Western Blotting Bulletin, Rev A
LIT234
Instruction Manual, Zeta-Probe Blotting Membranes, Rev C