Sequi-Blot PVDF Membrane

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Sequi-Blot PVDF (for Protein Sequencing)

Overview

Bio-Rad Protein Transfer Membrane Offerings
Protein Membranes

Bio-Rad's PVDF membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

Immun-Blot® PVDF Membrane
  • Ideal for chemiluminescent and colorimetric western blots
  • Retains target protein very strongly during protein transfer but also reduces nonspecific protein binding
Immun-Blot® Low Fluorescence PVDF Membrane
  • Same benefits as standard PVDF membranes plus minimal autofluorescence and high sensitivity
  • Best choice for multiplex fluorescent immunoblotting
Sequi-Blot™ PVDF Membrane
  • Ideal for protein sequencing: retains target protein very strongly during protein transfer
  • Provides the binding capacity to sequence even low-abundance samples
Nitrocellulose Membrane, 0.45 µm
  • Proven medium for western, northern, and Southern blotting
  • 45 µm pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers
  • 0.2 µm pore size is optimized for transferring nucleic acids and low molecular–weight proteins
Nitrocellulose Membrane, 0.2 µm
Supported Nitrocellulose Membrane, 0.45 µm
  • Dense 100% nitrocellulose cast on both sides of an inert polyester support
  • Easy to handle, wets readily, and does not have the brittleness often associated with unsupported nitrocellulose
Supported Nitrocellulose Membrane, 0.2 µm
PVDF Membranes

Ideal for:

  • Protein blotting
    (Immun-Blot® PVDF membrane)
  • Protein sequencing
    (Sequi-Blot™ PVDF membrane)

 

Comparison of Membranes

Superior retention of proteins by Sequi-Blot™ PVDF membrane.

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.

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Sequi-Blot™ PVDF membrane is ideal for protein sequencing: it retains target protein very strongly during protein transfer and provides the binding capacity to sequence even low-abundance samples.

Features and Benefits

  • Mechanical strength and chemical resistance provide ideal substrate for solvents involved in protein sequencing
  • Available in three formats:
    • Convenient precut membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Protein transfer from SDS-PAGE gels to membrane
  • Protein sequencing of target protein

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

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Sequi-Blot PVDF Membrane

1620184
Pkg of 1 roll, 0.2 μm, 26 cm x 3.3 m, bulk membrane for protein sequencing; binding capacity 170–200 μg/cm2

0 1620184 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0184_0921120407770.xml 1620184 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jdl0t1v7 2017-11-14 07:08:24 true true internet/product_SKU jdl0t1v7 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0184_0921120407770.xml LSR 2019-05-04 20:20:22 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556001556174 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:20:22 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0184_0921120407770.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0184_0921120407770.xml 1620184-sequi-blot-pvdf-membrane 1620184 Sequi-Blot PVDF Membrane Sequi-Blot PVDF Membrane Pkg of 1 roll, 0.2 μm, 26 cm x 3.3 m, bulk membrane for protein sequencing; binding capacity 170–200 μg/cm<sup>2</sup> Pkg of 1 roll, 0.2 μm, 26 cm x 3.3 m, bulk membrane for protein sequencing; binding capacity 170–200 μg/cm2 Pkg of 1 roll, 0.2 μm, 26 cm x 3.3 m, bulk membrane for protein sequencing; binding capacity 170–200 μg/cm<sup>2</sup> /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0184_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0184_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/162-0184_feature.jpg Sequi-Blot PVDF Membrane #162-0184 RepEx Sequi-Blot PVDF Membrane #162-0184 Pkg of 1 roll, 0.2 μm, 26 cm x 3.3 m, bulk membrane for protein sequencing; binding capacity 170–200 μg/cm2 1620184, sequi-blot, pvdf, membrane, 0.2 um, bulk, roll, protein, sequencing 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 ,VN <p>This Sequi-Blot&trade; PVDF membrane is packaged as a 26 cm wide, 3.3 m long bulk roll. The pore size is 0.2 &micro;m and binding capacity is 170&ndash;200 &micro;g/cm<sup>2</sup>.</p> <p>Sequi-Blot PVDF membrane is ideal for protein sequencing because it retains all protein during protein transfer and provides the binding capacity required to sequence even low-abundance samples.</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 162-0184_0921120407770_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//162-0184_0921120407770_en.html 1800 Material type PVDF 1 0 1991 Pore size, &micro;m 0.2 1 0 1660 Format Roll 1 0 1570 Dimensions, cm 26 x 3,300 1 0 false $409.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620184&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620184&CartQuote=QT&quantity= 1199 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620184&AddToDefaultWishList=true&quantity= 41105300 409.00 1620184
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Sequi-Blot PVDF Membrane

1620182
Pkg of 10, 0.2 μm, 20 x 20 cm, precut membrane for protein sequencing; binding capacity 170–200 μg/cm2

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Sequi-Blot PVDF Membrane (1620184)
Sequi-Blot PVDF Membrane

1620181
Pkg of 10, 0.2 μm, 15 x 15 cm, precut membrane for protein sequencing; binding capacity 170–200 μg/cm2

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Sequi-Blot PVDF Membrane (1620184)
Sequi-Blot PVDF Membrane

1620180
Pkg of 10, 0.2 μm, 10 x 15 cm, precut membrane for protein sequencing; binding capacity 170–200 μg/cm2

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Sequi-Blot PVDF Membrane (1620184)
Sequi-Blot PVDF Membrane

1620186
Pkg of 10, 0.2 μm, 7 x 8.4 cm, precut membrane for protein sequencing; binding capacity 170–200 μg/cm2

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Sequi-Blot PVDF Membrane (1620184)
Sequi-Blot PVDF/Filter Paper Sandwiches

1620236
Pkg of 20, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Criterion and Criterion XT gels

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Sequi-Blot PVDF Membrane (1620184)
Sequi-Blot PVDF/Filter Paper Sandwiches

1620237
Pkg of 50, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Criterion and Criterion XT gels

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • Sequi-Blot PVDF Membrane (1620184)
(Discontinued) Sequi-Blot PVDF/Filter Paper Sandwiches

1620216
Pkg of 20, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Mini-PROTEAN® and Ready Gel® precast gels

This product is no longer available. Please contact technical support for more information on replacement product.
Sequi-Blot™ PVDF/Filter Paper Sandwiches

1620217
Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Mini-PROTEAN® and Ready Gel® precast gels

9 1620217 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0217_0921120407684.xml 1620217 /default/main/bio-rad/WORKAREA/default 09/21/09 12:04 PM en_US jdl0t1uv 2017-11-14 07:08:24 true true internet/product_SKU jdl0t1uv templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0217_0921120407684.xml LSR 2019-05-04 19:50:56 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556001556473 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 19:50:56 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0217_0921120407684.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/162-0217_0921120407684.xml 1620217-sequi-blot-pvdf-filter-paper-sandwiches 1620217 Sequi-Blot PVDF/Filter Paper Sandwiches Sequi-Blot&trade; PVDF/Filter Paper Sandwiches Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Mini-PROTEAN<sup>&reg;</sup> and Ready Gel<sup>&reg;</sup> precast gels Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Ready Gel/Mini-PROTEAN gels /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/162-0217_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/162-0217_view.jpg Sequi-Blot PVDF/Filter Paper Sandwiches #162-0217 ,VN RepEx Sequi-Blot PVDF/Filter Paper Sandwiches #162-0217 Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Mini-PROTEAN and Ready Gel precast gels 1620217, sequi-blot, pvdf/filter, paper, sandwiches, pvdf, filter, 0.2 um, precut, preassembled, membrane, protein, sequencing, mini-protean, mini, protean, ready, gel 09/21/09 12:04 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>This Sequi-Blot&trade; PVDF membrane package contains 50 sheets of 7 x 8.5 cm precut, preassembled blotting membrane and filter paper sandwiches, which fit Ready Gel<sup>&reg;</sup> and Mini-PROTEAN<sup>&reg;</sup> precast gels. The pore size is 0.2 &micro;m and binding capacity is 170&ndash;200 &micro;g/cm<sup>2</sup>.</p> <p>Sequi-Blot PVDF membrane is ideal for protein sequencing because it retains all protein during protein transfer and provides the binding capacity required to sequence even low-abundance samples. The precut and preassembled blotting membrane and filter paper sets are designed to simplify western blotting setup.</p> /webroot/web/html/lsr/products/SKUs/autopopulated/ 162-0217_0921120407684_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//162-0217_0921120407684_en.html Material type 1800 PVDF and filter paper 1 0 Pore size, &micro;m 1991 0.2 1 0 Format 1660 Precut membrane and filter paper 1 0 Dimensions, cm 1570 7 x 8.5 1 0 false $520.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620217&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620217&CartQuote=QT&quantity= 1199 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1620217&AddToDefaultWishList=true&quantity= 41105300 520.00 1620217
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Number Description Options
2895 Protein Blotting Guide, Ver C Click to download
2212 Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C Click to download
LIT-240 Instruction Manual, Sequi-Blot PVDF Click to download

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