Overview
Description
Description
The ReadyPrep protein extraction kit (membrane II) applies sodium carbonate extraction methods (Fujiki et al. 1982, Molloy et al. 2000) to fractionate and isolate membrane proteins.
The ReadyPrep protein extraction kit (membrane II):
- Isolates complex proteins that contain more than two transmembrane domains or those that are resistant to extraction by the ReadyPrep protein extraction (membrane I) kit
- Requires ultracentrifugation
- Includes a strongly chaotropic, IEF-compatible rehydration/sample buffer that contains the detergent ASB-14 to efficiently solubilize proteins
References
Fujiki Y et al. (1982). Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J Cell Biology 93, 97–102.
Molloy MP et al. (2000). Proteomic analysis of the Escherichia coli outer membrane. Eur J Biochem 267, 2871–2881.
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10 preps, includes 2 x 5 ml 2-D Rehydration/Sample Buffer 1, 50 ml lysis buffer, 125 ml membrane protein concentrating reagent, 0.6 ml 200 mM tributylphosphine (TBP), 1 storage vial, instruction manual; for 10 extractions of 100–200 mg of cells or tissue