ReadyPrep™ Protein Extraction Kit (Cytoplasmic/Nuclear)

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ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear)


The ReadyPrep protein extraction kit (cytoplasmic/nuclear) prepares fractions enriched in cytoplasmic and nuclear proteins from eukaryotic samples, such as cultured cells and tissues.

The ReadyPrep protein extraction kit (cytoplasmic/nuclear):

  • Employs a specially formulated buffer and differential centrifugation to isolate intact nuclei (Dignam et al. 1983, Zerivitz and Akusjarvi 1989)
  • Contains a strongly chaotropic extraction buffer to efficiently solubilize nuclear proteins
  • Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that may interfere with IEF

References
Dignam JD et al. (1983). Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acid Res 11, 1475–1489.

Zerivitz K and Akusjarvi G (1989). An improved nuclear extract preparation method. Gene Anal Tech 6, 101–109.

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ReadyPrep™ Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps

1632089
50 preps, includes 50 ml cytoplasmic protein extraction buffer (CPEB), 50 ml protein solubilization buffer, 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 50 mg of cells or tissue

0 1632089 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2089_0921120738879.xml 1632089 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US juso9msb 2018-02-01 00:53:48 true true internet/product_SKU juso9msb templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2089_0921120738879.xml LSR 2019-05-04 16:23:50 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556909733691 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:23:50 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2089_0921120738879.xml templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2089_0921120738879.xml 1632089-readyprep-protein-extraction-kit-cytoplasmic-nuclear-50-preps 1632089 ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) ReadyPrep&trade; Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps 50 preps, includes 50 ml cytoplasmic protein extraction buffer (CPEB), 50 ml protein solubilization buffer, 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 50 mg of cells or tissue 50 preps, protein preparation and fractionation kit for cytoplasmic and nuclear proteins from eukaryotic samples 50 preps, includes 50 ml cytoplasmic protein extraction buffer (CPEB), 50 ml protein solubilization buffer, 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 50 mg of cells or tissue /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2089_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2089_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2089_feature.jpg ReadyPrep&trade; Protein Extraction Kit (Cytoplasmic/Nuclear), 50 preps preview ,VN RepEx 163-2089 50 preps, protein preparation and fractionation kit for cytoplasmic and nuclear proteins from eukaryotic samples 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Protein-Sample-Preparation/Protein-Fractionation N 0 <p>Use the ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) to enrich for both cytoplasmic and nuclear proteins. The kit separates cytoplasmic proteins from intact nuclei and then solubilizes nuclei.</p> <ul> <li>Employs a specially formulated buffer and differential centrifugation to isolate intact nuclei </li> <li>Contains a strongly chaotropic extraction buffer to efficiently solubilize nuclear proteins</li> <li>Includes the components of the ReadyPrep 2-D Cleanup Kit to remove extraction buffer that may interfere with IEF</li> <li>Extracted proteins are suitable for IEF, SDS-PAGE, and western blotting</li> </ul> <p>The ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) uses a buffer/differential centrifugation protocol to generate enriched cytoplasmic fractions and intact nuclei (Dignam et al. 1983, Zerivitz and Akusjarvi 1989). The nuclei are treated with a strongly chaotropic extraction buffer to efficiently solubilize the nuclear proteins. The cytoplasmic fraction is processed with the included <a href="/en-us/sku/1632130-readyprep-2-d-cleanup-kit-for-ief-50-preps?ID=1632130">ReadyPrep 2-D Cleanup Kit</a> to remove IEF-inhibiting compounds.&nbsp; Although this kit is directed towards the preparation of nuclear and cytoplasmic protein samples for 2-D gel electrophoresis, the fractions are suitable for SDS-PAGE, western blotting, and other applications.</p> <h2 class="descHeader">Kit Components</h2> <p>For 50 extractions of 50 mg of cells or tissue:<strong></strong></p> <ul> <li>Cytoplasmic protein extraction buffer (CPEB), 50 ml</li> <li>Protein solubilization buffer (PSB), 25 g</li> <li>3PSB diluent, 30 ml</li> <li>ReadyPrep 2-D Cleanup Kit (<a href="/en-us/sku/1632130-readyprep-2-d-cleanup-kit-for-ief-50-preps?ID=1632130">1632130</a>)</li> <li>Instruction manual</li> </ul> <p>Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="200" valign="top"><strong>ReadyPrep Kit for Fractionation by Cellular Location</strong></td> <td style="text-align: left;" valign="top"><strong>Proteins Targeted</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear)</td> <td style="text-align: left;" valign="top">Cytoplasmic and nuclear</td> <td style="text-align: center;" valign="top">1632089</td> </tr> <tr> <td valign="top">ReadyPrep Protein Extraction Kit (Membrane I)</td> <td style="text-align: left;" valign="top">Membrane-anchored, with one or two transmembrane domains, and hydrophobic</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632088-readyprep-protein-extraction-kit-membrane-i-50-preps?ID=1632088">1632088</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">ReadyPrep Protein Extraction Kit (Membrane II)</td> <td style="text-align: left;" valign="top">More than two transmembrane domains and/or proteins resistant to Membrane I Kit</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632084-readyprep-protein-extraction-kit-membrane-ii-10-preps?ID=1632084">1632084 </a></td> </tr> <tr> <td valign="top">ReadyPrep Protein Extraction Kit (Signal)</td> <td style="text-align: left;" valign="top">Associated with lipid rafts</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632087-readyprep-protein-extraction-kit-signal-50-preps?ID=1632087">1632087</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li>ReadyPrep Kits for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility</a></li> <li>Aurum&trade; Ion Exchange Mini Kits for <a href="/en-us/category/fractionation-charge">Fractionation by Charge</a></li> <li>Rotofor<sup>&reg;</sup> Liquid-Phase IEF Cells for <a href="/en-us/category/fractionation-pi">Fractionation by pI</a></li> <li>Model 491 Prep Cell and Mini Prep Cell for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility </a></li> <li><a href="/en-us/product/readyprep-2-d-starter-kit">ReadyPrep&trade; 2-D Starter Kit</a></li> <li><a href="/en-us/product/protean-i12-ief-system">PROTEAN<sup>&reg;</sup> i12&trade; IEF System</a></li> <li><a href="/en-us/product/readystrip-ipg-strips">ReadyStrip&trade; IPG Strips</a></li> <li>ReadyPrep Reduction-Alkylation Kit (<a href="/en-us/sku/1632090-readyprep-reduction-alkylation-kit-100-preps?ID=1632090">1632090</a>)</li> <li><a href="/en-us/product/powerpac-hv-high-voltage-power-supply">PowerPac&trade; HV High-Voltage Power Supply</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/electrophoresis-chambers">Electrophoresis Chambers</a></li> <li><a href="/en-us/category/2-d-electrophoresis?ID=6916a228-0f95-4b26-a37a-5e4afe76add7-buffers-reagents">2-D Electrophoresis Buffers and Reagents</a></li> <li><a href="/en-us/product/ampholytes">Ampholytes</a></li> <li><a href="/en-us/category/tube-gel-ief-systems">Tube Gel IEF Systems</a></li> <li><a href="/en-us/category/imaging">Gel Imaging Systems</a></li> <li><a href="/en-us/product/2-d-electrophoresis-workflow">2-D Electrophoresis Workflow</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>ReadyPrep Protein Extraction Kit (Cytoplasmic Nuclear) <a href="/webroot/web/pdf/lsr/literature/MS4110147A.pdf" target="_blank">Instruction Manual</a></li> <li>Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion <a href="/webroot/web/pdf/lsr/literature/Bulletin_3096.pdf" target="_blank">Brochure</a></li> <li>ReadyStrip IPG Strip <a href="/webroot/web/pdf/lsr/literature/4006166H.pdf" target="_blank">Instruction Manual</a></li> <li>PROTEAN i12 IEF System <a href="/webroot/web/pdf/lsr/literature/Bulletin_6097A.pdf" target="_blank">Brochure</a></li> <li>Host Cell Protein (HCP) Workflow <a href="/webroot/web/pdf/lsr/literature/Bulletin_6493.pdf" target="_blank">Quick Guide</a></li> </ul> <h2 class="descHeader">References</h2> <p>Dignam JD et al. (1983). Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acid Res 11, 1475&ndash;1489. PMID: <a href="http://www.ncbi.nlm.nih.gov/pubmed/6828386">6828386</a> <br />Zerivitz K and Akusjarvi G (1989). An improved nuclear extract preparation method. Gene Anal Tech 6, 101&ndash;109. PMID: <a href="http://www.ncbi.nlm.nih.gov/pubmed/2555292">2555292</a></p> /webroot/web/html/lsr/products/SKUs/autopopulated 1632089.html /webroot/web/html/lsr/products/SKUs/autopopulated/1632089.html false $508.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632089&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632089&CartQuote=QT&quantity= 1432 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632089&AddToDefaultWishList=true&quantity= 41115800 508.00 Product detail page 1632089
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4110147 Instruction Manual, ReadyPrep™ Protein Extraction Kit (Cytoplasmic/Nuclear), Rev A Click to download
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download

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