ReadyPrep™ Protein Extraction Kit (Membrane I)

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ReadyPrep Protein Extraction Kit (Membrane I)


The ReadyPrep protein extraction kit (membrane I) uses temperature-dependent phase partitioning and Triton X-114 detergent (Bordier 1981, Santoni et al. 2000) to prepare fractions that are enriched in membrane and hydrophobic proteins.

The ReadyPrep protein extraction kit (membrane I):

  • Isolates proteins associated with membranes or containing two transmembrane domains
  • Does not require the use of ultracentrifugation or density gradients
  • Contains a strongly chaotropic extraction buffer to efficiently solubilize proteins
  • Includes the components of the ReadyPrep 2-D cleanup kit to remove compounds from the extraction buffer that might interfere with IEF

 

References
Bordier C (1981). Phase separation of integral membrane proteins in Triton X-114. J Biol Chem 256, 1604–1607.
Santoni V et al. (2000). Membrane proteomics: Use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoresis properties. Electrophoresis 21, 3329–3344.

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ReadyPrep™ Protein Extraction Kit (Membrane I), 50 preps

1632088
50 preps, includes 50 ml membrane protein extraction buffer 1 (M1), 50 ml membrane protein extraction buffer 1 (M1), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 25–50 mg of cells or tissue

0 1632088 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2088_0921120739021.xml 1632088 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US jcjsxy9u 2017-11-14 07:08:59 true true internet/product_SKU jcjsxy9u templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2088_0921120739021.xml LSR 2019-05-04 16:23:38 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556909733638 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:23:38 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2088_0921120739021.xml templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2088_0921120739021.xml 1632088-readyprep-protein-extraction-kit-membrane-i-50-preps 1632088 ReadyPrep Protein Extraction Kit (Membrane I) ReadyPrep&trade; Protein Extraction Kit (Membrane I), 50 preps 50 preps, includes 50 ml membrane protein extraction buffer 1 (M1), 50 ml membrane protein extraction buffer 1 (M1), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 25–50 mg of cells or tissue 50 preps, protein preparation and fractionation kit for most membrane proteins, temperature dependent differential solubilization with Triton X-114 50 preps, includes 50 ml membrane protein extraction buffer 1 (M1), 50 ml membrane protein extraction buffer 1 (M1), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, 1 ReadyPrep 2-D Cleanup Kit (1632130), instruction manual; for 50 extractions of 25–50 mg of cells or tissue /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2088_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2088_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2088_feature.jpg ReadyPrep&trade; Protein Extraction Kit (Membrane I), 50 preps preview ,VN RepEx 163-2088 50 preps, protein preparation and fractionation kit for most membrane proteins, temperature dependent differential solubilization with Triton X-114 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Protein-Sample-Preparation/Protein-Fractionation N 0 <p>Use the ReadyPrep Protein Extraction Kit (Membrane I) for simple, rapid and reproducible preparation of cellular protein fractions highly enriched in membrane and hydrophobic proteins. Protein isolated using this kit can be used for many applications including 2-D gel analysis, SDS-PAGE, and western blotting.</p> <ul type="disc"> <li>Isolates proteins containing 1&ndash;2 transmembrane domains, membrane-associated proteins, and hydrophobic proteins </li> <li>Protocol can be used with a wide variety of biological samples</li> <li>Does not require the use of ultracentrifugation or density gradients</li> <li>Contains a strongly chaotropic extraction buffer to efficiently solubilize proteins</li> <li>Includes the components of the ReadyPrep 2-D Cleanup Kit to remove constituents of the extraction buffer that can interfere with IEF</li> </ul> <p>The ReadyPrep Protein Extraction Kit (Membrane I) separates membrane proteins by temperature-dependent phase partitioning using Triton X-114 detergent (Bordier 1981, Santoni et al. 2000). Proteins anchored to the membrane or proteins containing one or two transmembrane domains are efficiently partitioned to the detergent-rich phase. Cytoplasmic proteins partition into the aqueous phase. An insoluble pellet contains more complex membrane proteins. Proteins extracted into the two phases are treated using the included <a href="/en-us/sku/1632130-readyprep-2-d-cleanup-kit-for-ief-50-preps?ID=1632130">ReadyPrep 2-D Cleanup Kit</a> to remove kit components that interfere with IEF. Pellets are then efficiently solubilized using the strongly chaotropic extraction buffer. The solubilized proteins can then be immediately used in 2-D gel electrophoresis or other applications.</p> <h2 class="descHeader">Kit Components</h2> <p>For 50 extractions of 25&ndash;50 mg of cells or tissue:<strong></strong></p> <ul> <li>Membrane protein extraction buffer 1 (M1), 50 ml</li> <li>Membrane protein extraction buffer 2 (M2), 50 ml</li> <li>Protein solubilization buffer (PSB), 25 g</li> <li>PSB diluent, 30 ml</li> <li>ReadyPrep 2-D Cleanup Kit (<a href="/en-us/sku/1632130-readyprep-2-d-cleanup-kit-for-ief-50-preps?ID=1632130">1632130</a>)</li> <li>Instruction manual</li> </ul> <p>Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="200" valign="top"><strong>ReadyPrep Kit for Fractionation by Cellular Location</strong></td> <td style="text-align: left;" valign="top"><strong>Proteins Targeted</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear)</td> <td style="text-align: left;" valign="top">Cytoplasmic and nuclear</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632089-readyprep-protein-extraction-kit-cytoplasmic-nuclear-50-preps?ID=1632089">1632089</a></td> </tr> <tr> <td valign="top">ReadyPrep Protein Extraction Kit (Membrane I)</td> <td style="text-align: left;" valign="top">Membrane-anchored, with one or two transmembrane domains, and hydrophobic</td> <td style="text-align: center;" valign="top">1632088</td> </tr> <tr class="pd_colorbackground"> <td valign="top">ReadyPrep Protein Extraction Kit (Membrane II)</td> <td style="text-align: left;" valign="top">More than two transmembrane domains and/or proteins resistant to Membrane I Kit</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632084-readyprep-protein-extraction-kit-membrane-ii-10-preps?ID=1632084">1632084 </a></td> </tr> <tr> <td valign="top">ReadyPrep Protein Extraction Kit (Signal)</td> <td style="text-align: left;" valign="top">Associated with lipid rafts</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632087-readyprep-protein-extraction-kit-signal-50-preps?ID=1632087">1632087</a></td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li>ReadyPrep Kits for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility</a></li> <li>Aurum&trade; Ion Exchange Mini Kits for <a href="/en-us/category/fractionation-charge">Fractionation by Charge</a></li> <li>Rotofor<sup>&reg;</sup> Liquid-Phase IEF Cells for <a href="/en-us/category/fractionation-pi">Fractionation by pI</a></li> <li>Model 491 Prep Cell and Mini Prep Cell for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility </a></li> <li><a href="/en-us/product/readyprep-2-d-starter-kit">ReadyPrep&trade; 2-D Starter Kit</a></li> <li><a href="/en-us/product/protean-i12-ief-system">PROTEAN<sup>&reg;</sup> i12&trade; IEF System</a></li> <li><a href="/en-us/product/readystrip-ipg-strips">ReadyStrip&trade; IPG Strips</a></li> <li>ReadyPrep Reduction-Alkylation Kit (<a href="/en-us/sku/1632090-readyprep-reduction-alkylation-kit-100-preps?ID=1632090">1632090</a>)</li> <li><a href="/en-us/product/powerpac-hv-high-voltage-power-supply">PowerPac&trade; HV High-Voltage Power Supply</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/electrophoresis-chambers">Electrophoresis Chambers</a></li> <li><a href="/en-us/category/2-d-electrophoresis?ID=6916a228-0f95-4b26-a37a-5e4afe76add7-buffers-reagents">2-D Electrophoresis Buffers and Reagents</a></li> <li><a href="/en-us/product/ampholytes">Ampholytes</a></li> <li><a href="/en-us/category/tube-gel-ief-systems">Tube Gel IEF Systems</a></li> <li><a href="/en-us/category/imaging">Gel Imaging Systems</a></li> <li><a href="/en-us/product/2-d-electrophoresis-workflow">2-D Electrophoresis Workflow</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>ReadyPrep Protein Extraction Kit (Membrane I) <a href="/webroot/web/pdf/lsr/literature/MS4110148A.pdf" target="_blank">Instruction Manual</a></li> <li>Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion <a href="/webroot/web/pdf/lsr/literature/Bulletin_3096.pdf" target="_blank">Brochure</a></li> <li>ReadyStrip IPG Strip <a href="/webroot/web/pdf/lsr/literature/4006166H.pdf" target="_blank">Instruction Manual</a></li> <li>PROTEAN i12 IEF System <a href="/webroot/web/pdf/lsr/literature/Bulletin_6097A.pdf" target="_blank">Brochure</a></li> <li>Host Cell Protein (HCP) Workflow <a href="/webroot/web/pdf/lsr/literature/Bulletin_6493.pdf" target="_blank">Quick Guide</a></li> </ul> <h2 class="descHeader">References</h2> <p>Bordier C (1981). Phase separation of integral membrane proteins in Triton X-114. J Biol Chem 256, 1604&ndash;1607. PMID: <a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=6257680">6257680</a><br /> Santoni V et al. (2000). Membrane proteomics: Use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoresis properties. Electrophoresis 21, 3329&ndash;3344. PMID: <a href="http://www.ncbi.nlm.nih.gov/pubmed/11079553">11079553</a></p> /webroot/web/html/lsr/products/SKUs/autopopulated 1632088.html /webroot/web/html/lsr/products/SKUs/autopopulated/1632088.html false $508.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632088&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632088&CartQuote=QT&quantity= 1432 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632088&AddToDefaultWishList=true&quantity= 41115800 508.00 1632088
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3103 Removal of Abundant Myofilament Proteins From Rabbit Myocardium Using the ReadyPrep Protein Extraction Kit (Membrane I) Click to download
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