ReadyPrep™ Protein Extraction Kit (Signal)

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ReadyPrep Protein Extraction Kit (Signal)

Overview

Fractionation of Proteins by Cellular Location

Differences in 2-D patterns using ReadyPrep signal (A), membrane I (B), and membrane II (C) kits. Mouse liver samples were extracted using the recommended protocols for each kit. Purified protein (~450 µg) was focused on 17 cm pH 3–10 nonlinear ReadyStrip IPG strips and run on 8–16% PROTEAN II precast gels for the second dimension. Overall spot patterns differ for A, B, and C even though all three kits isolate membrane proteins, indicating each kit isolates different types of membrane proteins.

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The ReadyPrep protein extraction kit (signal) employs differential solubilization at 4°C in the presence of Triton X-100 to isolate proteins associated with lipid rafts. These proteins are often involved in intracellular membrane trafficking and signaling pathways and include the following: glycosylphosphatidylinositol (GPI)-anchored proteins, caveolin, caveolae (caveolin-associated proteins), acylated tyrosine kinases, heterotrimeric G proteins, and some proteins with transmembrane domains. These proteins are resolubilized in the protein solubilization buffer (PSB), which contains nondetergent sulfobetaine 201 (NDSB 201) along with urea, thiourea, and CHAPS, and which is compatible with a variety of downstream applications, including IEF.

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ReadyPrep™ Protein Extraction Kit (Signal), 50 preps

1632087
50 preps, includes 50 ml signal protein extraction buffer 1 (S1), 50 ml signal protein extraction buffer 2 (S2), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, instruction manual; for 50 extractions of 25–50 mg of cells or tissue

0 1632087 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2087_0921120739195.xml 1632087 /default/main/bio-rad/WORKAREA/default 09/21/09 12:07 PM en_US juso9ms9 2018-02-01 00:53:48 true true internet/product_SKU juso9ms9 templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2087_0921120739195.xml LSR 2019-05-04 16:23:46 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1556909733594 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 16:23:46 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2087_0921120739195.xml templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2087_0921120739195.xml 1632087-readyprep-protein-extraction-kit-signal-50-preps 1632087 ReadyPrep Protein Extraction Kit (Signal) ReadyPrep&trade; Protein Extraction Kit (Signal), 50 preps 50 preps, includes 50 ml signal protein extraction buffer 1 (S1), 50 ml signal protein extraction buffer 2 (S2), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, instruction manual; for 50 extractions of 25–50 mg of cells or tissue 50 preps, protein preparation and fractionation kit for signal proteins, Triton X-100 solubilization 50 preps, includes 50 ml signal protein extraction buffer 1 (S1), 50 ml signal protein extraction buffer 2 (S2), 25 g protein solubilization buffer (PSB), 30 ml PSB diluent, instruction manual; for 50 extractions of 25–50 mg of cells or tissue /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2087_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2087_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2087_feature.jpg ReadyPrep&trade; Protein Extraction Kit (Signal), 50 preps preview ,VN RepEx 163-2087 50 preps, protein preparation and fractionation kit for signal proteins, Triton X-100 solubilization 09/21/09 12:07 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Protein-Sample-Preparation/Protein-Fractionation N 0 <p>&nbsp;Choose the ReadyPrep Protein Extraction Kit (Signal) to enrich for signaling proteins. This kit uses differential solubilization at 4&deg;C in the presence of Triton X-100 to isolate proteins associated with lipid rafts.</p> <ul> <li>Isolate proteins involved in intracellular membrane trafficking and signaling pathways</li> <li>After fractionation, proteins are easily solubilized in the supplied buffer</li> <li>Solubilized proteins can be used immediately in IEF, SDS-PAGE and other applications</li> </ul> <p>Many proteins located within lipid rafts are resistant to solubilization at 4&deg;C by Triton X-100 and other nonionic detergents. The ReadyPrep Protein Extraction Kit (Signal) utilizes a proprietary mixture of nonionic detergents to extract and remove hydrophilic and soluble proteins, leaving behind the signal proteins in a detergent-insoluble fraction.</p> <p>Proteins that can be isolated using this kit include</p> <ul> <li>Gycosylphosphatidylinositol (GPI)-anchored proteins</li> <li>Caveolin and other caveolae-associated proteins</li> <li>Acylated tyrosine kinases</li> <li>Heterotrimeric G-proteins</li> <li>Some proteins with transmembrane domains</li> </ul> <h2 class="descHeader">Kit Components</h2> <p>For 50 extractions of 25&ndash;50 mg of cells or tissue:<strong></strong></p> <ul> <li>Signal protein extraction buffer 1 (S1), 50 ml</li> <li>Signal protein extraction buffer 2 (S2), 50 ml</li> <li>Protein solubilization buffer (PSB), 25 g</li> <li>PSB diluent, 30 ml</li> <li>Instruction manual </li> </ul> <p>Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="200" valign="top"><strong>ReadyPrep Kit for Fractionation by Cellular Location</strong></td> <td style="text-align: left;" valign="top"><strong>Proteins Targeted</strong></td> <td style="text-align: center;" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear)</td> <td style="text-align: left;" valign="top">Cytoplasmic and nuclear</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632089-readyprep-protein-extraction-kit-cytoplasmic-nuclear-50-preps?ID=1632089">1632089</a></td> </tr> <tr> <td valign="top">ReadyPrep Protein Extraction Kit (Membrane I)</td> <td style="text-align: left;" valign="top">Membrane-anchored, with one or two transmembrane domains, and hydrophobic</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632088-readyprep-protein-extraction-kit-membrane-i-50-preps?ID=1632088">1632088</a></td> </tr> <tr class="pd_colorbackground"> <td valign="top">ReadyPrep Protein Extraction Kit (Membrane II)</td> <td style="text-align: left;" valign="top">More than two transmembrane domains and/or proteins resistant to Membrane I Kit</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1632084-readyprep-protein-extraction-kit-membrane-ii-10-preps?ID=1632084">1632084 </a></td> </tr> <tr> <td valign="top">ReadyPrep Protein Extraction Kit (Signal)</td> <td style="text-align: left;" valign="top">Associated with lipid rafts</td> <td style="text-align: center;" valign="top">1632087</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li>ReadyPrep Kits for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility</a></li> <li>Aurum&trade; Ion Exchange Mini Kits for <a href="/en-us/category/fractionation-charge">Fractionation by Charge</a></li> <li>Rotofor<sup>&reg;</sup> Liquid-Phase IEF Cells for <a href="/en-us/category/fractionation-pi">Fractionation by pI</a></li> <li>Model 491 Prep Cell and Mini Prep Cell for <a href="/en-us/category/fractionation-solubility">Fractionation by Solubility </a></li> <li><a href="/en-us/product/readyprep-2-d-starter-kit">ReadyPrep&trade; 2-D Starter Kit</a></li> <li><a href="/en-us/product/protean-i12-ief-system">PROTEAN<sup>&reg;</sup> i12&trade; IEF System</a></li> <li><a href="/en-us/product/readystrip-ipg-strips">ReadyStrip&trade; IPG Strips</a></li> <li>ReadyPrep Reduction-Alkylation Kit (<a href="/en-us/sku/1632090-readyprep-reduction-alkylation-kit-100-preps?ID=1632090">1632090</a>)</li> <li><a href="/en-us/product/powerpac-hv-high-voltage-power-supply">PowerPac&trade; HV High-Voltage Power Supply</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/electrophoresis-chambers">Electrophoresis Chambers</a></li> <li><a href="/en-us/category/2-d-electrophoresis?ID=6916a228-0f95-4b26-a37a-5e4afe76add7-buffers-reagents">2-D Electrophoresis Buffers and Reagents</a></li> <li><a href="/en-us/product/ampholytes">Ampholytes</a></li> <li><a href="/en-us/category/tube-gel-ief-systems">Tube Gel IEF Systems</a></li> <li><a href="/en-us/category/imaging">Gel Imaging Systems</a></li> <li><a href="/en-us/product/2-d-electrophoresis-workflow">2-D Electrophoresis Workflow</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>ReadyPrep Protein Extraction Kit (Signal) <a href="/webroot/web/pdf/lsr/literature/MS4110143A.pdf" target="_blank">Instruction Manual</a></li> <li>Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion <a href="/webroot/web/pdf/lsr/literature/Bulletin_3096.pdf" target="_blank">Brochure</a></li> <li>ReadyStrip IPG Strip <a href="/webroot/web/pdf/lsr/literature/4006166H.pdf" target="_blank">Instruction Manual</a></li> <li>PROTEAN i12 IEF System <a href="/webroot/web/pdf/lsr/literature/Bulletin_6097A.pdf" target="_blank">Brochure</a></li> <li>Host Cell Protein (HCP) Workflow <a href="/webroot/web/pdf/lsr/literature/Bulletin_6493.pdf" target="_blank">Quick Guide</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1632087.html /webroot/web/html/lsr/products/SKUs/autopopulated/1632087.html false $262.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632087&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632087&CartQuote=QT&quantity= 1432 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632087&AddToDefaultWishList=true&quantity= 41115800 262.00 1632087
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4110149 Instruction Manual, ReadyPrep™ Protein Extraction Kit (Signal), Rev A Click to download

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