iScript™ Reverse Transcription Supermix, 500 x 20 µl rxns, 2 ml #1708841BUN



500 x 20 µl reactions, 20 x 100 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl2, stabilizers; 4 x 50 reactions of no-RT control supermix



Choose iScript Reverse Transcription Supermix for RT-qPCR for fast synthesis of first-strand cDNA for direct use in real-time qPCR. This single-tube supermix contains all the components for reverse transcription except RNA template.

Key Features and Benefits

  • Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
  • Contains RNase H+ MMLV reverse transcriptase and has a linear range of 1 µg–1 pg total input RNA
  • Amplify cDNA for genes with low expression levels
  • Fast 26-minute 1-tube cDNA synthesis protocol
  • 50 reactions of no-RT controls for optional detection of gDNA amplicon contamination

Packaging Options

Kit Size Supermix Volume, µl Catalog Number
25 x 20 µl reactions 100 (1 x 100 µl) 1708840
100 x 20 µl reactions 400 (4 x 100 µl) 1708841
500 x 20 µl reactions 2000 (20 x 100 µl) 1708841BUN

See the iScript Reverse Transcription Supermix product page for more information and documentation.

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