StarBright Blue 520 Fluorescent Secondary Antibodies

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Overview

Choose StarBright Blue 520 Fluorescent Secondary Antibodies, conjugated to a high-yield fluorophore, for ultra-sensitive fluorescence detection with very low background of single or multiple proteins in one blot without stripping or reprobing.

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Description

Description

StarBright Blue 520 Secondary Antibodies, with an excitation maximum at 440 nm and emission maximum at 520 nm, are ideal for fluorescent western blotting. The StarBright fluorophore is a fluorescent nanoparticle that gives a signal that is considerably brighter than conventional dyes.

StarBright Blue 520 Conjugated Goat Anti-Mouse and Goat Anti-Rabbit Fluorescent Secondary Antibodies are ideal for the detection of a single-target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. StarBright Blue 520 Fluorescent Secondary Antibodies can be used with far-red emission StarBright Blue 700 Antibodies as well as traditional fluorescent detection reagents like Alexa Fluor and DyLight Conjugated Antibodies. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or with hFAB Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc MP Imaging System, permitting detection of up to three proteins in a single blot.

Features and Benefits

  • Easy multiplexing — can be used with traditional fluorescent antibodies or with Bio-Rad's StarBright Blue 700 Fluorescent Secondary Antibodies to detect multiple proteins on the same blot
  • High sensitivity — StarBright Blue 520 Fluorescent Secondary Antibodies (Exmax/Emmax = 440 nm/520 nm) have 2–4 fold higher sensitivity compared to traditional green emitters like Alexa Fluor 488 or DyLight 488 Antibodies
  • Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
  • Low nonspecific binding — the StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies

Applications and Uses

StarBright Blue 520 Fluorescent Secondary Antibodies can be used for multiplex fluorescent western blotting with Bio-Rad's ultra-sensitive near IR StarBright Blue 700 Antibodies and the hFAB Rhodamine Anti-Housekeeping Antibodies as described in the table below.

Recommended Fluorophore Combinations

Targets Primary Target
(Fluorophore A)
Second Target
(Fluorophore B)
Third Target
(Fluorophore C)
1 StarBright Blue 700
2 StarBright Blue 700 hFAB Rhodamine HKP* Antibody (α-actin/tubulin/GAPDH)
3 StarBright Blue 700 StarBright Blue 520 hFAB Rhodamine HKP Antibody (α-actin/tubulin/GAPDH)

* Stain-free gels can be used for total protein normalization in place of or in addition to housekeeping proteins (HKP).

Product Options

Products Cross-Adsorbed Species Catalog Number
Bovine Goat Human Rabbit Mouse Rat
StarBright Blue 520 Goat Anti-Mouse IgG, 80 µl   12005867
StarBright Blue 520 Goat Anti-Mouse IgG, 400 µl   12005866
StarBright Blue 520 Goat Anti-Rabbit IgG, 80 µl   12005870
StarBright Blue 520 Goat Anti-Rabbit IgG, 400 µl   12005869

Related Products

Related Categories

  • DyLight Fluorescent Antibodies

Supporting Documents

Online Resources

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starbright-blue-520-goat-anti-mouse-igg-80-ul-12005867-tn.jpg
12005867
Pkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 80 µl
starbright-blue-520-goat-anti-rabbit-igg-400-ul-12005866-tn.jpg
12005866
Pkg of 1, lyophilized goat anti-mouse IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 400 µl
starbright-blue-520-goat-anti-rabbit-igg-80-ul-12005870-tn.jpg
12005870
Pkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 80 µl
starbright-blue-520-goat-anti-rabbit-igg-400-ul-12005869-tn.jpg
12005869
Pkg of 1, lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 520 Fluorophore; reconstitute to 400 µl

Documents

Documents

Number Description Options
6911
Fluorescent Western Blotting Antibodies Flier
10000079534
Instruction Manual, StarBright Blue Fluorescent Secondary Antibodies
10000078050
Comprehensive Guide to Fluorescent Western Blotting with StarBright and hFAB™ Rhodamine Antibodies