StarBright Blue 520 Fluorescent Secondary Antibodies
Choose StarBright Blue 520 Fluorescent Secondary Antibodies, conjugated to a high-yield fluorophore, for ultra-sensitive fluorescence detection with very low background of single or multiple proteins in one blot without stripping or reprobing.
Overview
StarBright Blue 520 Secondary Antibodies, with an excitation maximum at 440 nm and emission maximum at 520 nm, are ideal for fluorescent western blotting. The StarBright fluorophore is a fluorescent nanoparticle that gives a signal that is considerably brighter than conventional dyes.
StarBright Blue 520 Conjugated Goat Anti-Mouse and Goat Anti-Rabbit Fluorescent Secondary Antibodies are ideal for the detection of a single-target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. StarBright Blue 520 Fluorescent Secondary Antibodies can be used with far-red emission StarBright Blue 700 Antibodies as well as traditional fluorescent detection reagents like Alexa Fluor and DyLight Conjugated Antibodies. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or with hFAB Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc MP Imaging System, permitting detection of up to three proteins in a single blot.
Features and Benefits
- Easy multiplexing — can be used with traditional fluorescent antibodies or with Bio-Rad's StarBright Blue 700 Fluorescent Secondary Antibodies to detect multiple proteins on the same blot
- High sensitivity — StarBright Blue 520 Fluorescent Secondary Antibodies (Exmax/Emmax = 440 nm/520 nm) have 2–4 fold higher sensitivity compared to traditional green emitters like Alexa Fluor 488 or DyLight 488 Antibodies
- Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
- Low nonspecific binding — the StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies
Applications and Uses
StarBright Blue 520 Fluorescent Secondary Antibodies can be used for multiplex fluorescent western blotting with Bio-Rad's ultra-sensitive near IR StarBright Blue 700 Antibodies and the hFAB Rhodamine Anti-Housekeeping Antibodies as described in the table below.
Recommended Fluorophore Combinations
| Targets | Primary Target (Fluorophore A) |
Second Target (Fluorophore B) |
Third Target (Fluorophore C) |
| 1 | StarBright Blue 700 | — | — |
| 2 | StarBright Blue 700 | hFAB Rhodamine HKP* Antibody (α-actin/tubulin/GAPDH) | — |
| 3 | StarBright Blue 700 | StarBright Blue 520 | hFAB Rhodamine HKP Antibody (α-actin/tubulin/GAPDH) |
* Stain-free gels can be used for total protein normalization in place of or in addition to housekeeping proteins (HKP).
Product Options
| Products | Cross-Adsorbed Species | Catalog Number | |||||
| Bovine | Goat | Human | Rabbit | Mouse | Rat | ||
| StarBright Blue 520 Goat Anti-Mouse IgG, 80 µl | • | • | • | • | • | 12005867 | |
| StarBright Blue 520 Goat Anti-Mouse IgG, 400 µl | • | • | • | • | • | 12005866 | |
| StarBright Blue 520 Goat Anti-Rabbit IgG, 80 µl | • | • | • | • | • | 12005870 | |
| StarBright Blue 520 Goat Anti-Rabbit IgG, 400 µl | • | • | • | • | • | 12005869 | |
Related Products
- StarBright Blue 700 Fluorescent Secondary Antibodies
- hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies
- PrecisionAb Western Blotting Primary Antibodies
- Mini-PROTEAN TGX Stain-Free Precast Gels
- ChemiDoc MP Imaging System
Related Categories
- DyLight Fluorescent Antibodies
Supporting Documents
- Fluorescent Western Blotting Antibodies Flier
- StarBright Blue Fluorescent Secondary Antibodies Instruction Manual
Online Resources
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