Digital PCR Library Quantification Kits

Image
digital-pcr-library-quantification-kits-detail.png

Overview

Bio-Rad's digital PCR kits allow accurate quantification of DNA libraries before next-generation sequencing (NGS) on Illumina RNA-Seq and Ion Torrent AmpliSeq Platforms.

Read full description Ordering Information

Description

Description

Precise quantification of next-generation sequencing (NGS) libraries is critical for the efficient use of NGS platforms. Bio-Rad's digital PCR platform and library quantification kits can be easily incorporated into the NGS library preparation workflow to precisely quantify and balance sequencing libraries on the Illumina or Ion Torrent Platforms.

The ddPCR library quantification assay generates data plots that are rich with qualitative library information, a feature not available with other current methodologies.

When paired with the QX200 Droplet Digital PCR System, these kits allow accurate quantification and provide a qualitative measure of the DNA library prior to sequencing on Illumina (186-3040).

Features of Digital PCR Library Quantification Kits

  • Contain all the necessary components to create droplets and quantify NGS libraries
  • Provide information about library quality, such as adapter dimers, and indicate library insert size
  • Provide more efficient and consistent loading of libraries for sequencing runs
  • Enable balancing of pooled library samples
  • Optimize the use of consumables, labor, and instrument time for the highest productivity with NGS

Related Products

Bio-Rad offers additional digital PCR supermixes including:

Additional Equipment

These digital PCR assays require the use of the following instruments and accessories:

Specifications

Specifications

Storage at –20°C
Guaranteed up to 18 months in a constant temperature freezer
(multiple freeze/thaws not recommended)

Ordering

Ordering

items

Use the filters below to refine results!

ddpcr-library-quantification-kit-for-ilumina-truseq-186-3040-thumb.jpg
1863040
200 x 20 µl reactions, includes ddPCR Library Quantification Assay and ddPCR Supermix for Probes (No dUTP), for quantification of Illumina TruSeq libraries using the QX200™/QX100™ ddPCR™ Systems

Documents

Documents

Number Description Options
6402
ddPCR Library Quantification Kit for Illumina TruSeq Flier, Rev B
10031986
ddPCR Library Quantification Kit for Illumina TruSeq Product Insert, Rev C
6343
QX100 Droplet Digital PCR System for Accurate Quantification of Illumina TruSeq Libraries, Rev B
6490
ddPCR Library Quantification Kit for Ion Torrent Flier, Rev A
10033630
ddPCR Library Quantification Kit for Ion Torrent Product Insert, Rev B
6495
Bio-Rad’s ddPCR™ Library Quantification Kit for Ion Torrent Enables Accurate Library Quantification and Excellent Balancing of Pooled Libraries, Rev A
6407
Droplet Digital PCR Applications Guide
6423
Droplet Digital PCR: Ultra-Sensitive Validation Technology for RNA-Seq Application Note, Rev A
[ Add to Cart (Free) ]
10023997
Instruction Manual, PX1 PCR Plate Sealer, Rev A