Expert Coffee Chat Webinar: Chromatography and Protein Purification

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Our customers asked our Bio-Rad application scientists about the current issues most relevant to their lab-scale and process chromatography projects. A lively discussion was had about sample preparation, system maintenance, various purification method tips, coronavirus research, and more. Watch the video of this session and read the questions and answers below.

Air Date: May 6, 2020

We had so many great questions asked by the audience. Watch the whole video, or quickly jump to a specific question:

  • 00:10 Introduction
  • 02:40 How is chromatography utilized in the development and manufacturing of vaccines?
  • 06:20 How do researchers utilize protein chromatography to study novel viruses like MERS, SARS, and now SARS-CoV-2?
  • 09:20 What are some of your suggestions to help me increase yield and purity?
  • 14:10 How are lysates usually prepared before applied to the column, filtration, centrifugation etc. and how does the scale affect this procedure?
  • 20:00 What types of resins do you think undergraduate students should learn about/use in teaching labs to get them ready for biomedical research? Or, what are the most commonly used resins used in biomedical research?
  • 24:25 Have you any tips for peptide purification rather than protein purification?
  • 27:00 Is Expanded Bed Chromatography still being used in industries?
  • 28:18 You all mentioned a lot about resin chromatography? Do you offer any porous membrane for chromatography?
  • 29:14 I'm having problems with protein precipitation. What are your recommendations for aiding in solubility?
  • 32:36 Have you any experience with twin-column (tandem) chromatography?
  • 35:58 I am working on a new protein target. Where do I start? Are there any columns or resins you would recommend?
  • 40:20 What maintenance should I be performing on my instrument?
  • 44:25 I left the lab in a hurry to shelter in place, what would you recommend I do to my system before I use it again?
  • 47:48 Closing

Chromatography Questions Answered by Our Experts

In this section, you will find audience questions and thorough answers from our application scientists grouped by common chromatography topics.

Sample Preparation

 How are sample lysates usually prepared before applied to the column, filtration, centrifugation, etc., and how does the scale affect this procedure?

 I am having problems with protein precipitation. What are your recommendations for aiding in solubility?

Chromatography Methods

 What are some of your suggestions to help me increase yield and purity?

 Do you have any tips for peptide purification rather than protein purification?

 Is Expanded Bed Chromatography still being used in industry?

 Do you have any experience with twin-column (tandem) chromatography?

 I am working on a new protein target. Where do I start? Are there any columns or resins would you recommend?

 Do you recommend a "polish" purification before standard purification / exchange? If so, what would this look like?

 When should I choose chromatography compared to capillary electrophoresis (CE) for protein purification?

 What solvents are compatible with my chromatography system?

Chromatography System Maintenance

 What maintenance should I be performing on my instrument?

 I left the lab in a hurry to shelter-in-place, what would you recommend I do to my system before I use it again?

 Any tips for cleaning clogged columns?

 How long can I keep the gel filtration column in running buffer instead of 20% EtOH until the next run?

Coronavirus Research

 How is chromatography utilized in the development and manufacturing of vaccines?

 How do researchers utilize protein chromatography to study novel viruses like MERS, SARS, and now SARS-CoV-2?

Miscellaneous Chromatography

 What types of resins do you think undergraduate students should learn about/use in teaching labs to get them ready for biomedical research? Or, what are the most commonly used resins used in biomedical research?

 You all mentioned a lot about resin chromatography? Do you offer any porous membrane for chromatography?

Coffee Chat with Bio-Rad Application Scientists

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Chromatography and Protein Purification Products

  • NGC Chromatography Systems

    The family of NGC Medium-Pressure Chromatography Systems fit your throughput requirements when purifying biomolecules for analytical- and preparative-scale applications.

  • Chromatography Resins

    Bio-Rad offers a selection of resins for separation by ion exchange, hydroxyapatite and fluoroapatite, affinity, size exclusion (gel filtration), and hydrophobic interaction chromatography.

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