Reliance One-Step Multiplex RT-qPCR Supermix

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Overview

Reliance One-Step Multiplex Supermix is a ready-to-use, room-temperature stable, 4x reaction master mix optimized for sensitive amplification of up to 5 targets in a single reaction.
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Description

Description

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Use the Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.

Features and Benefits



Exceptional Performance of 5-Target Multiplex

Reliance One-Step Multiplex Supermix enables researchers to get the most data out of their samples. Optimally formulated with a novel proprietary Reliance Reverse Transcriptase and robust DNA polymerases blend, this supermix amplifies up to 5 targets across a broad linear dynamic range from 1 pg to 1 µg of RNA input with maximum sensitivity and efficiency.




Unparalleled Room Temperature Stability for Up To 24 Hours

Reliance One-Step Multiplex Supermix is developed with high-throughput researchers in mind. Whether you want to queue up plates ahead of time or use an automated workflow, Reliance One-Step Multiplex Supermix will maintain its performance even after 24 hour incubation of assembled reactions.




Efficient Co-detection of RNA and DNA Targets

Reliance One-Step Multiplex Supermix is designed to efficiently detect both RNA and DNA targets in the same reaction. Whether samples are tested for both RNA and DNA viruses at the same time or are run with internal controls, Reliance One-Step Multiplex Supermix reliably amplifies multiple targets regardless of their nature.

View Application Note (PDF 143KB)




Streamline Your Workflow

4x Reliance One-Step Multiplex Supermix is Formulated with:

  • Optimal blend of Reliance chimeric reverse transcriptase* and high-performance DNA polymerases including Sso7d fusion DNA polymerase**
  • 4X reaction buffer containing dNTPs, RNase inhibitor, and other reaction optimizing components
  • Reaction stabilizers
  • Patented*** universal reference dye

Applications and Uses

  • Gene expression analysis
  • Viral/pathogen detection (RNA viruses, DNA viruses, bacterial viruses, and fungal viruses)
  • Diagnostic research
  • High-throughput screening (small molecules, drug discovery, dosage experiment, CRISPR screening, and toxicology study)

Instrument Compatibility

Reliance One-Step Multiplex Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0

Choose the Right One-Step RT-qPCR Product for Your Needs

  Reliance One-Step Multiplex Supermix iTaq Universal One-Step Probe Kit
Key Features
  • Sensitive, efficient amplification of up to 5 targets
  • Room temperature stable up to 24 hours
  • Reliable amplification of up to 2 targets
  • Compatible with a fast protocol
Ideal for
  • Viral/pathogen detection in high-multiplex reaction
  • High throughput screening
  • Viral/pathogen detection
  • Singleplex and duplex reactions

Supporting Documents

  • Amplification Reagents and Plastics Brochure
  • SingleShot Cell Lysis Kit Instruction Manual
  • One-Step Multiplex RT-qPCR Using PrimePCR Probe Assays Protocol
  • Sensitive Codetection of Viral-Pathogen RNA and DNA Using One-Step Multiplex RT-qPCR Application Note

Online Resources

What is Real-Time PCR (qPCR)?

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* Patent pending
** U.S. patents 6,627,424; 7,541,170; and 7,560,260.
*** US patent 9,493,824

Specifications

Specifications

Product format
Single-tube, ready-to use supermix
Concentration 
4x concentrated
Detection chemistry
Probes
Multiplex capability
Up to 5 targets
Assembled reaction stability
Up to 24 hours
Inhibitor tolerance
Tested with ethanol and hematin. Also compatible with up to 10 µl of crude lysates
5’ to 3’ exonuclease activity of the enzyme(s)
Yes
Hot-start, activation time
Yes, 10 min
Sample input
1 pg to 1 µg
Sensitivity
10 copies in a 5-plex reaction
Reference dye
Tented universal dye preblended in the supermix
Instrument compatibility
Both ROX-dependent (high- and low-) and -independent instruments
Guaranteed shelf life at –20°
12 months
Shelf life at 4°C (after thawing)
1 week

Ordering

Ordering

items

Use the filters below to refine results!

12010176_th.jpg
12010176
1 ml (1 x 1 ml vial), 4x one-step RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 200 x 20 μl rxns
12010220_th.jpg
12010220
5 ml (5 x 1 ml vials), 4x one-step RT-qPCR supermix, contains Reliance reverse transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 1,000 x 20 μl rxns
12010221_th.jpg
12010221
10 ml (2 x 5 ml vials), 4x one-step RT-qPCR supermix, contains Reliance reverse transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 2,000 x 20 μl rxns

Related Prod

Related Prod

single-shot-cell-lysis-kit-172-5080-thumb.jpg
1725080

Pkg of 1, kit includes 5 ml (1 x 5 ml) of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions.

single-shot-cell-lysis-kit-172-5081-thumb.jpg
1725081
Pkg of 1, kit includes 25 ml (5 x 5 ml) of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions
cfx-automation-system-II-184-5075-thumb.jpg
1845075
Plate handler and barcode scanner, mounting plate, automation software; does not include CFX Real-Time PCR Detection Systems

Documents

Documents

Number Description Options
10000108800
Reliance One-Step Multiplex Supermix Product Insert
7197
Protocol for One-Step Multiplex RT-qPCR Analysis Using PrimePCR Probe Assays and Reliance One-Step Multiplex Supermix
7175
Reliance One-Step Multiplex Supermix Product Information Sheet, Ver B
7178
Amplification: Real-Time PCR Reagents and Kits Brochure
7196
Sensitive Codetection of Viral-Pathogen RNA and DNA Using One-Step Multiplex RT-qPCR
7275
Do More with Less: Five Steps to Great Results Using One-Step Multiplex RT-qPCR
10000112808
User Manual, Singleshot Cell Lysis Kit
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
6629
High-Throughput Real-Time PCR Solutions Brochure
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]