1-Step Quantitative Reverse Transcription PCR (RT-qPCR) from RNA

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Overview

iTaq Universal One-Step RT-qPCR Kits provide high PCR efficiency in sensitive SYBR<sup>&reg;</sup> Green or probe assays, even with difficult targets and challenging samples. Ideal for high-throughput RT-qPCR screening.

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Description

Description

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iTaq Universal One-Step RT-qPCR Kits are a fast and convenient solution for real-time PCR using the powerful combination of iScript RNase H+ MMLV reverse transcriptase and hot-start iTaq DNA Polymerase in one reaction, providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity, and inhibitor tolerance without affecting performance.

Available for SYBR®Green and probe-based qPCR assays, iTaq Universal One-Step Kits are ideal for low-to mid-throughput real-time PCR screening and validation. A simplified work flow and reduced cycling times enable screening and validation of the greatest number of samples and targets in the shortest period of time.

Features and Benefits of iTaq Universal One-Step RT-qPCR Kits

  • Enhanced efficiency, specificity, and sensitivity— our patented inhibitor reducer* prevents interference of the DNA polymerase by the RT enzyme during cDNA replication
  • Compatible with all real-time PCR instruments — our universal reference dye enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
  • Superior gene expression results under various cycling conditions — our robust formulation delivers consistent performance in standard or fast cycling conditions, primer concentrations, sample and target types, and temperature ranges, allowing cost and time savings
  • Obtain superior results with singleplex and multiplex reactions — advanced formulation enables the simultaneous amplification of up to two targets, resulting in higher data precision with fewer pipet steps and reduced sample usage

Instrument Compatibility

iTaq Universal One-Step RT-qPCR Kits are compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses of iTaq Universal One-Step RT-qPCR Kits

  • Gene expression analysis
  • Multiplexing
  • Absolute quantification
  • Mutation detection
  • Pathogen detection
  • Viral detection
  • Genetically modified organisms (GMO) characterization
  • Genetic profiling

Related Categories

Supporting Documentation

Online Resources

* U.S. patent 8,338,094.

Specifications

Specifications

Product format
Two-tube system
Kit content
iScript reverse transcriptase + 2x iTaq PCR reaction mix
Detection chemistry
SYBR Green or probes
Multiplex capability (for probes only)
Duplex (triplex possible depending on the abundance of the targets)
Inhibitor tolerance
Tested with ethanol. Also compatible with up to 4 µl of crude lysates
5’ to 3’ exonuclease activity of the enzyme(s)
Yes
Hot-start, activation time
Yes, 1–3 min
Sample input
100 fg to 100 ng
Sensitivity
10 copies in a duplex reaction
Reference dye
Patented universal dye preblended in the iTaq reaction mix
Instrument compatibility
Both ROX-dependent (high- and low-) and -independent instruments
Product expiration (after shipping date)
12 months
RT storage at –20°C (after shipping date)
12 months
Reaction mix storage at 4°C (after thawing)
Up to 3 months
Customer guarantee (minimum usage time) at –20°C
12 months

Ordering

Ordering

items

Use the filters below to refine results!

itaq-universal-sybr-green-one-step-kit-1ml-172-5150-thumb.jpg
1725150
100 x 20 µl reactions, 1 ml (1 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water
itaq-universal-sybr-green-one-step-kit-5ml-172-5151-thumb.jpg
1725151
500 x 20 µl reactions, 5 ml (5 x 1 ml), one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript™ reverse transcriptase; and nuclease-free water
itaq-universal-probes-one-step-kit-172-5140-thumb.jpg
1725140
100 x 20 µl reactions, 1 ml (1 x 1 ml) 2x one-step reaction mix, containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water
itaq-universal-probes-one-step-kit-172-5141-thumb.jpg
1725141
500 x 20 µl reactions, 5 ml (5 x 1 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; iScript reverse transcriptase; and nuclease-free water
12013250
5,000 x 20 µl reactions, 50 ml (1 x 50 ml) 2x one-step reaction mix containing iTaq DNA polymerase, buffers, and ROX normalization dyes; 2.5 ml (1 x 2.5 ml) iScript reverse transcriptase; and 45 ml (1 x 45 ml) nuclease-free water

Documents

Documents

Number Description Options
10032048
iTaq™ Universal SYBR® Green One-Step Kit Product Insert, Rev B
10032046
iTaq Universal Probes One-Step Kit Product Insert, Rev B
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
7178
Amplification: Real-Time PCR Reagents and Kits Brochure
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
6290
PrimePCR Assays and Panels Brochure, Rev D
6516
Better, Faster, and Less Expensive Gene Expression Analysis Using Multiplex One-Step RT-qPCR: A Case Study of Colorectal Cancer, Rev A