Protein Blotting Detection and Imaging

Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (immunodetection) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.

Related Topics: Protein Blotting Methods, Membranes and Blotting Papers, Transfer Buffers, and Transfer Conditions.

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Page Contents
Protein Detection Systems

Protein Detection Systems

Protein detection systems and their sensitivity.


Number Description Options
Protein Blotting Guide, Ver C
Western Blotting Detection Reagents Brochure, Rev F


Number Description Options
Number Description Options
6216 Detection Buffer Formulations Click to download
6217 Total Protein Detection Click to download
6218 Blot Stripping & Reprobing Click to download
6219 Immunodetection Click to download