Monoclonal Antibody Purification

While most monoclonal antibody (mAb) purification for process development begins with Protein A, you will often need intermediate and polishing steps to achieve full separation of your therapeutic from host cell proteins (HCPs), aggregates, and other contaminants.

At Bio-Rad, you will find a full range of quality resins that can take you from process development into manufacturing. Even better, you will also find a team of experienced bioprocess scientists ready to help you overcome any purification challenges. Whether you are purifying a monoclonal antibody, bispecific antibody, an antibody-drug conjugate, or any antibody format, you will find our selection of unique selectivity resins will meet your biomolecule purification needs.

Find a Resin for Every Stage of Purification

• Capture

  If you are wanting to move away from Protein A and want an alternative like ion exchange, you will find an effective resin for your purification development.

  Learn about our capture purification resins »

• Intermediate Polishing

  When your goal is to remove impurities like HCPs and aggregates, you are most likely looking for an ion exchange resin. We offer anion and cation exchange resins that offer high productivity and the ability to maintain performance after multiple cleaning-in-place (CIP) cycles.

  Learn about selecting the optimal resin for aggregate removal »

• Final Polishing

  Ion exchange chromatography is a great choice for removing closely related molecules and mixed-mode resins can effectively purify with high yield and achieve purity in a two-step purification workflow. Our mixed-mode library includes CHT, a cationic metal affinity resin, Nuvia cPrime, a hydrophobic cationic exchange resin, and Nuvia aPrime 4A, a hydrophobic anionic exchange resin.

  Learn about our polish purification resins »

Resources

View all of our process chromatography resources »

Browse a collection of resources, including resin information and sample request, technical videos and tutorials, application notes, and strategy guides to help with your purification success, from development to commercialization.

Documents

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  Selecting Chromatography Resins for Antibody Purification

  Learn how to select optimal chromatography resins for monoclonal antibody (mAb) purification with our handy guide.

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  Optimizing mAb Purity and Recovery with Mixed-Mode Resins

  Discover how mixed-mode approaches can streamline your purification workflow, increase efficiency and recovery of antibodies.

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  6 Benefits of Prepacked Process-Scale Chromatography Columns

  Learn why biopharma has turned to prepacked chromatography columns and their benefits to biotherapeutic manufacturing.

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  Biosimilar Production with IEX and Mixed-Mode Resins

  Learn how process economics is improved more than fourfold for biosimilar production with an ion exchange and mixed-mode purification workflow.

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  Antibody Purification Using a Hydrophobic AEX Mixed-Mode Resin

  Learn how an optimized hydrophobic anion-exchange (AEX) mixed-mode resin can enable exceptional purification and recovery of monoclonal antibodies.

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  Monoclonal Antibody Purification Guide

  Optimize your antibody purification strategy with our antibody purification guide for selecting resins ideal for capture, intermediate, and polish.

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  Simplifying Antibody Purification: Alternative to 3-Step Process

  Learn how a two-step mixed-mode chromatography approach can simplify antibody purification without compromising purity or yield.

References

1. Tanford C, Reynolds J. Nature’s Robots: A History of Proteins. OUP Oxford; 2003. https://books.google.com/books?id=Vg5H7InfABEC