Monoclonal Antibody
Purification

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Resin Overview | Custom Seminars | Applications | Resources |
Leverage Our Novel Resins and Proven Expertise
Delivering more than just resins, at Bio-Rad you’ll find robust solutions for challenging purifications by working with our experienced monoclonal antibody purification support scientists.
Start a Conversation with a SpecialistWhile most monoclonal antibody (mAb) purification for process development begins with Protein A, you will often need intermediate and polishing steps to achieve full separation of your therapeutic from host cell proteins (HCPs), aggregates, and other contaminants.
At Bio-Rad, you will find a full range of quality resins that can take you from process development into manufacturing. Even better, you will also find a team of experienced bioprocess scientists ready to help you overcome any purification challenges. Whether you are purifying a monoclonal antibody, bispecific antibody, an antibody-drug conjugate, or any antibody format, you will find our selection of unique selectivity resins will meet your biomolecule purification needs.
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Did you know?
The first proteins were described in 1789 by the French chemist Antoine Fourcroy, a contemporary of Antoine Lavoisier. Fourcroy described albumin, fibrin, and gelatin.1
Find a Resin for Every Stage of Purification
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Capture
If you are wanting to move away from Protein A and want an alternative like ion exchange, you will find an effective resin for your purification development.
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Intermediate Polishing
When your goal is to remove impurities like HCPs and aggregates, you are most likely looking for an ion exchange resin. We offer anion and cation exchange resins that offer high productivity and the ability to maintain performance after multiple cleaning-in-place (CIP) cycles.
Learn about selecting the optimal resin for aggregate removal »
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Final Polishing
Ion exchange chromatography is a great choice for removing closely related molecules and mixed-mode resins can effectively purify with high yield and achieve purity in a two-step purification workflow. Our mixed-mode library includes CHT, a cationic metal affinity resin, Nuvia cPrime, a hydrophobic cationic exchange resin, and Nuvia aPrime 4A, a hydrophobic anionic exchange resin.
Custom Seminars
Our team of purification scientists is available to assist you with method and process development. Request a custom seminar to discuss your purification needs with our experienced scientists.
Applications
Removing Aggregates of an Acidic Monoclonal Antibody with CHT Ceramic Hydroxyapatite XT Media
Learn how the CHT XT Resin can minimize aggregate content during mAb purification.
Purification of Antibodies with Nuvia aPrime 4A Hydrophobic Anion Exchange Resin
Discover how Nuvia aPrime 4A Resin improves process productivity and economy.
Purification of a Recombinant Monoclonal Antibody
Learn how a non-affinity-based purification workflow for rituximab biosimilar can provide better process economics.
A Non-Affinity Chromatography Resin Alternative for Capture Purification of Antibodies
Find out how the Nuvia S Ion Exchange Resin serves as a viable alternative in antibody purification workflows.
Improved Process Economics of Humira Biosimilar Purification with Ion Exchange and Mixed Mode Resins
Read how the IEX-based workflow with a mixed-mode resin polish step can help achieve greater than 99% purity of a biosimilar.
Development of a Non-Affinity Based Purification Platform for Neutral/Basic IgMs
See our non-affinity-based purification protocol that constitutes a new platform process for diagnostic IgM purification.
Resources
View all of our process chromatography resources
Browse a collection of resources to help with your purification success, from development to commercialization.
Documents
Minimizing On-Column Monoclonal Antibody Aggregate Formation
Review the importance of screening multiple resins during mAb purification process development.
Selecting the Optimal Resins for Monoclonal Antibody Process Purification
Determine your optimal mAb purification strategy with our broad range of resins.
References
- Tanford C, Reynolds J. Nature’s Robots: A History of Proteins. OUP Oxford; 2003. https://books.google.com/books?id=Vg5H7InfABEC
Ready to learn how Bio-Rad can accelerate your method and process development for monoclonal antibody purification? Our scientists are here to help.
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