en-usLUSN6ZE8ZIon Exchange ChromatographyIon Exchange Chromatography/webroot/web/html/lsr/solutions/technologies/chromatography<p>Ion exchange chromatography is a technique for separating compounds based on their
net charge. Ion exchange chromatography media contain negatively or positively charged
functional groups covalently bound to a solid support, yielding either a cation or
anion exchanger, respectively. Charged compounds are adsorbed and retained by an ion
exchanger having the opposite charge, whereas compounds that are neutral or have the
same charge as the media pass through the void volume and are eluted from the column.
The binding of the charged compounds is reversible, and adsorbed compounds are commonly
eluted with a salt or pH gradient. Ion exchange media are available in various particle
sizes, ionic forms, and purity ranges.</p>
<p>This section provides an overview of the selection of ion exchange chromatography
media based on factors such as the isoelectric point (pI) of the compounds to be separated
and the ionic form, porosity, and particle size of the media. The quick guide for
media selection below is based on the specific technique used for separation —
such as ion-exchange, hydroxyapatite, fluoroapatite, or affinity chromatography —
and the various media available from Bio-Rad for these techniques. To determine whether
ion exchange chromatography is the most suitable approach for your application and
to learn about other chromatographic methods that may be applicable to your purification
needs, visit our <a href="/evportal/destination/solutions?catID=LUSMIS7OP">Chromatography</a>
page.</p>
<p><strong>Related Topics:</strong> <a href="/evportal/destination/solutions?catID=LUSMJIDN">Affinity
Chromatography</a>, <a href="/evportal/destination/solutions?catID=LUSMV015">Size
Exclusion Chromatography</a>, <a href="/evportal/destination/solutions?catID=LUSN8F4VY">Hydrophobic
Interaction Chromatography</a>, <a href="/evportal/destination/solutions?catID=LUSN9AKG4">Mixed-Mode
Chromatography</a>, <a href="/evportal/destination/solutions?catID=LUSNA7ESH">Low
Pressure Chromatography Systems</a> and <a href="/evportal/destination/solutions?catID=LUSNE3C4S">Medium
Pressure Chromatography Systems</a>.</p>
What Molecules Can Be Purified by Ion Exchange Chromatography? <p><img src="/webroot/web/images/lsr/solutions/technologies/lab_scale_chromatography/chromatography/technology_detail/ion-exchange-chromatography-media-decision-tree-ion-exchange-chromatography.jpg"
alt="Flow chart for selecting ion exchange chromatography media" width="485" height="280"
/></p>
<div class="top"><a href="#helptop">Back to Top</a></div>
Ion Exchange Chromatography Media Selection Guide<table class="pd_table" border="0">
<tbody>
<tr>
<td class="bordertop" colspan="2" valign="top"> </td>
<td class="bordertop borderbttm" colspan="3" align="center" valign="top"><strong>Suitability**</strong></td>
<td class="bordertop" valign="top"> </td>
</tr>
<tr>
<td valign="bottom"><strong>Media Type</strong></td>
<td valign="bottom"><strong>Packaging<br /> Format*</strong></td>
<td valign="bottom"><strong>Analytical<br /> Scale</strong></td>
<td valign="bottom"><strong>Pilot/<br />Preparative<br />Scale</strong></td>
<td valign="bottom"><strong>Process<br /> Scale</strong></td>
<td valign="bottom"><strong>Application</strong></td>
</tr>
<tr>
<td class="bordertop" colspan="6" valign="top"><strong>Anion Exchange Chromatography
Media</strong></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=c8ee0998-3f05-4c43-9179-2b318ef5e15f">AG 1</a></td>
<td valign="top">B, GC</td>
<td valign="top">++++</td>
<td valign="top">++</td>
<td valign="top">+</td>
<td valign="top">Strong exchanger. Separation of low MW peptides, nucleotides, inorganic
ions using different crosslinkages; high selectivity for anions such as chloride;
gravity or low-pressure use</td>
</tr>
<tr>
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=9d70419d-626a-4ca1-9928-cc25738eff99">AG
MP-1M</a></td>
<td valign="top">B, GC</td>
<td valign="top">++++</td>
<td valign="top">++ +</td>
<td valign="top">+</td>
<td valign="top">Strong exchanger. Macroporous, equivalent to AG 1 for MW >1,000,000;
gravity or low pressure use</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b2125a69-a777-424f-8c10-99b453263a57">Bio-Rex
5</a> and <br /> <a href="http://www.bio-rad.com/prd/en/US/adirect/biorad?cmd=catProductDetail&vertical=LSR&productID=140-4341&vertical=LSR&parentCategoryGUID=dce910ad-f7ef-4500-99cc-5e7deb4197de">AG
4-X4</a></td>
<td valign="top">B, GC</td>
<td valign="top">++++</td>
<td valign="top">++</td>
<td valign="top"> </td>
<td valign="top">Weak exchanger. Used to remove organic acids from sugars; adsorption
of mineral acids; gravity or low-pressure use</td>
</tr>
<tr>
<td valign="top"><a href="/evportal/destination/commerce/sku_detail?productID=720-0009">UNO Q</a></td>
<td valign="top">MPC</td>
<td valign="top">++++</td>
<td valign="top"> </td>
<td valign="top"> </td>
<td valign="top">Strong exchanger. High-resolution biomolecule separation at high
flow rates; pH stability 2–12</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=963aeae1-7233-47c2-bd22-88dda61a39b1">Macro-Prep <br
/> High Q</a></td>
<td valign="top">B, C</td>
<td valign="top">+++</td>
<td valign="top">++++</td>
<td valign="top">+</td>
<td valign="top">Strong exchanger. High-capacity biomolecule separation; unique surface
chemistry allows contaminant removal; pH stability 1–10</td>
</tr>
<tr>
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=0366b503-2727-48bd-bfec-813ba77c5ff2">Macro-Prep
25 Q</a></td>
<td valign="top">B</td>
<td valign="top">++++</td>
<td valign="top">++++</td>
<td valign="top">+</td>
<td valign="top">Strong exchanger. Similar to Macro-Prep High Q but 25 μm particle
size allows higher-resolution separation; unique surface chemistry allows contaminant
removal; pH stability 1–10</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b67f362d-de39-4eee-9b66-775834614dd2">Macro-Prep
DEAE</a></td>
<td valign="top">B, C</td>
<td valign="top">+++</td>
<td valign="top">++++</td>
<td valign="top">+</td>
<td valign="top">Weak exchanger. High-capacity biomolecule separation; unique surface
chemistry allows contaminant removal; pH stability 1–10</td>
</tr>
<tr>
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=9f94b8bc-4b23-48da-b60b-b2b8570cabb9">UNOsphere
Q</a></td>
<td valign="top">B, C</td>
<td valign="top">++++</td>
<td valign="top">++++</td>
<td valign="top">++++</td>
<td valign="top">Strong exchanger. High-productivity, high-capacity biomolecule separation;
pH stability 1–14</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="/evportal/destination/product?catID=dcedd7d7-b864-4e48-8769-696e63ea21db">Aminex</a></td>
<td valign="top">HPLC</td>
<td valign="top">++++</td>
<td valign="top"> </td>
<td valign="top"> </td>
<td valign="top">High-pressure separation of carbohydrates, sugars, and small organic
molecules; delivers industry-standard performance (U.S. Pharmacopeia)</td>
</tr>
<tr>
<td class="bordertop" colspan="6" valign="top"><strong>Cation Exchange Chromatography
Media</strong></td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=0c308553-8af9-4991-9f81-660f2298b385">AG
50W</a></td>
<td valign="top">B, GC</td>
<td valign="top">++++</td>
<td valign="top">++</td>
<td valign="top">+</td>
<td valign="top">Strong exchanger. Lower degree of crosslinking, useful for peptide
and nucleotide separation; higher crosslinkages useful for small peptide and metals
separation or cation removal; gravity or low-pressure use</td>
</tr>
<tr>
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=0c308553-8af9-4991-9f81-660f2298b385">AG
MP-50</a></td>
<td valign="top">B, GC</td>
<td valign="top">++++</td>
<td valign="top">++</td>
<td valign="top">+</td>
<td valign="top">Strong exchanger. Macroporous equivalent to AG 50W for MW >1,000,000;
gravity or low pressure use</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=b565fec4-b7e7-40a3-89b4-3bccede7152c">Bio-Rex
MSZ 501</a></td>
<td valign="top">B</td>
<td valign="top">++++</td>
<td valign="top">++</td>
<td valign="top">++</td>
<td valign="top">Same as AG 50 but with more uniform bead size; large bead size (650
μm) makes it ideal for large-scale (column or batch) industrial applications</td>
</tr>
<tr>
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=029d7515-f0ae-4f0f-8274-1146e071de23">Bio-Rex
70</a></td>
<td valign="top">B</td>
<td valign="top">++++</td>
<td valign="top">++</td>
<td valign="top">++</td>
<td valign="top">Weak exchanger. High capacity for high MW (>1,000,000) solutes;
can be used for purification and fractionation of peptides, proteins, enzymes, and
other cationic molecules. Amenable to large-scale purification</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=6448ab3e-b96a-4162-9124-7b7d2330288e">Chelex 100</a></td>
<td valign="top">B, GC</td>
<td valign="top">++++</td>
<td valign="top">++ +</td>
<td valign="top">+</td>
<td valign="top">Weak exchanger. Chelating resin removes metals and is suitable for
PCR applications; can also be used for ultrapurification of buffers and ionic reagents;
gravity or lowpressure use. Available in molecular biology and biotechnology grades</td>
</tr>
<tr>
<td valign="top"><a href="/evportal/destination/commerce/sku_detail?productID=720-0029">UNO
S</a></td>
<td valign="top">MPC</td>
<td valign="top">++++</td>
<td valign="top"> </td>
<td valign="top"> </td>
<td valign="top">Strong exchanger. High-resolution biomolecule separation at high
flow rates; pH stability 2–12</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=5f0f3b17-a6bb-4eb8-b74f-d7184d5fbfd9">Macro-Prep
25 S</a></td>
<td valign="top">B</td>
<td valign="top">++++</td>
<td valign="top">++++</td>
<td valign="top">+</td>
<td valign="top">Strong exchanger. Similar to Macro-Prep High S, but 25 μm particle
size allows higher-resolution separation; unique surface chemistry allows contaminant
removal; pH stability 1–10</td>
</tr>
<tr>
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=24884a41-3f11-47f4-a4b5-6b8d6d408ae7">Macro-Prep<br
/> High S</a></td>
<td valign="top">B, C</td>
<td valign="top">+++</td>
<td valign="top">++++</td>
<td valign="top">+</td>
<td valign="top">Strong exchanger. High-capacity biomolecule separation; unique surface
chemistry allows contaminant removal; pH stability 1–10</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=8d374055-8d46-4b36-b72d-6a38c5ef1547">Macro-Prep
CM</a></td>
<td valign="top">B</td>
<td valign="top">+++</td>
<td valign="top">++++</td>
<td valign="top">+</td>
<td valign="top">Weak exchanger. High-capacity biomolecule separation; unique surface
chemistry<br /> allows contaminant removal; pH stability 1–10</td>
</tr>
<tr>
<td valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=71b79e5f-6fa5-4983-bc6b-35ee3bb23eb8">UNOsphere
S</a></td>
<td valign="top">B, C</td>
<td valign="top">++++</td>
<td valign="top">++++</td>
<td valign="top">++++</td>
<td valign="top">Strong exchanger. High-capacity biomolecule separation; pH stability
1–14</td>
</tr>
<tr class="pd_colorbackground">
<td valign="top">UNOsphere Rapid S</td>
<td valign="top">B, C</td>
<td valign="top">++++</td>
<td valign="top">++++</td>
<td valign="top">++++</td>
<td valign="top">Strong exchanger. Similar to UNOsphere S but with enhanced chemistries
to overcome the pH shift that occurs with conductivity transitions and faster equilibration
times; pH stability 1–14</td>
</tr>
<tr>
<td class="borderbttm" valign="top"><a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=L2DCMZ15">Nuvia
S</a></td>
<td class="borderbttm" valign="top">B, C</td>
<td class="borderbttm" valign="top">++++</td>
<td class="borderbttm" valign="top">++++</td>
<td class="borderbttm" valign="top">++++</td>
<td class="borderbttm" valign="top">Strong exchanger. Similar to UNOsphere S but surface
modification allows extremely high-capacity biomolecule separation; pH stability 1–14</td>
</tr>
</tbody>
</table>
<p class="caption">* B, bottle; C, cartridge (1 ml or 5 ml); GC, gravity column; SC,
spin column; HPLC, high-pressure column; MPC, medium-pressure column.</p>
<p class="caption">** +, low suitability; ++, moderate suitability; +++, suitable;
++++, high suitability.</p>
<div class="top"><a href="#helptop">Back to Top</a></div>
Ion Exchange Chromatography Media Selection<p>Selection of an ion exchange chromatography support depends on the properties of
the compounds to<br />be separated.</p>
<p><strong>Isoelectric Point of the Compound</strong> — for amphoteric compounds,
the pI of the compound and its stability at various pH values determine the separation
strategy. At a pH above its pI, the compound of interest will be negatively charged;
conversely, at a pH below its pI, the compound will be positively charged. Thus, when
the compound is stable at a pH above its pI, an anion exchange support is used. Conversely,
a cation exchange support is used when the compound is stable at a pH below its pI.
The operating pH also determines the type of exchanger to use. A strong ion exchange
support maintains its capacity over a wide pH range, whereas a weak one loses capacity
when the pH no longer matches the acid dissociation constant (pKa) of its functional
group.</p>
<p><img src="/webroot/web/images/lsr/solutions/technologies/lab_scale_chromatography/chromatography/technology_detail/ion-exchange-chromatography-media-selection-chart-ion-exchange-chromatography.jpg"
alt="Ion exchange chromatography media selection chart" width="285" height="208" /></p>
<p><strong> Ionic Form of the Media</strong> — most ion exchange media are available
in several ionic forms and may be converted from one form to another. The ionic form
of a support refers to the counterion presently adsorbed onto the support's functional
groups. Counterions exhibit specific selectivities for each support. The lower the
selectivity of a counterion toward the support, the more readily it is exchanged for
another ion of like charge. Consequently, the appropriate ionic form of the ion exchange
media will depend on the relative selectivity of the sample ion to be adsorbed. In
general, the ionic form should have a lower selectivity for the functional group than
the sample ion, so that the sample ion will displace the counterion and be adsorbed
onto the support. The sample ion can then be eluted by a second counterion with a
higher selectivity for the support.</p>
<p><strong>Media Porosity</strong> — the porosity of a support refers to the
total pore volume within the matrix of the support; greater pore volume equals higher
porosity. A very porous support may have either many small pores or a few large pores.
The exclusion limit of a support is defined as the size of the largest compound that
is able to enter the pores under a given set of conditions. Porous media with high
exclusion limits are recommended for high-molecular weight compounds such as proteins,
antibodies, and other biomolecules. Low- or high-porosity media with low exclusion
limits are recommended for the separation of low-molecular weight compounds such as
inorganic ions and organic acids. Some of the high-porosity media that Bio-Rad offers
include the UNO family of ion exchange media, <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=36286010-253e-4553-b13d-8b5917f8e93a">UNOsphere</a>,
<a href="/en-us/product/nuvia-s-resin?ID=L2DCMZ15">Nuvia</a>, <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=74b7a57e-0a19-4f8f-b641-aa099f513d42">Macro-Prep</a>,
and <a href="https://www.bio-rad.com/prd/en/US/adirect/biorad?cmd=catProductDetail&vertical=LSR&country=US&lang=en&productID=142-5822">Bio-Rex
70 media</a>. Low-porosity media include the <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=dce910ad-f7ef-4500-99cc-5e7deb4197de">AG</a>
and <a href="http://www.bio-rad.com/evportal/destination/commerce/product_detail?catID=6448ab3e-b96a-4162-9124-7b7d2330288e">Chelex
resins</a></p>
<p><strong>Media Particle Size</strong> — particle size is measured in micrometers,
with dry mesh or wet mesh designations. The wet particle diameter varies from support
to support and depends on the ionic form resulting from differences in the hydration
of the particles. Smaller particle sizes provide higher resolution and typically require
lower operational flow rates; larger particle sizes yield lower resolution but can
be operated at higher flow rates.</p>
<p>Bio-Rad also offers <a href="http://www.bio-rad.com/evportal/destination/product?catID=dce910ad-f7ef-4500-99cc-5e7deb4197de">molecular-biology-and
biotechnology-grade resins</a>, free of endo- and exonuclease activity and ligase
inhibitors as well as <a href="/en-us/category/analytical-grade-ion-exchange-resins?ID=6512cba2-a0c6-4062-915c-6d0df409bdec">technical-
and reactor-grade resins</a> suitable for power plant deionization and large-scale
cleanup of metal ions.</p>
<div class="top"><a href="#helptop">Back to Top</a></div>
2729UNOsphere™ Q Support Technical Data, Rev A2729/webroot/web/pdf/lsr/literature/Bulletin_2729.pdfLiteraturePDFApplication_Notes/webroot/web/images/general/icons/icon_pdf.gifUNOsphere™ Q Support Technical Data, Rev ANoUNOsphere™ Q Support Technical Data2729uno sphere, unosphere, ion exchange, anion, exchange, anion exchange, anex, iex, chromatography,
resin, bulletin 2729, lit2729, 156-0101, 156-0103, 156-0105, 156-0107, 1560101, 1560103,
1560105, 1560107
27244110109Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C4110109/webroot/web/pdf/lsr/literature/4110109C.pdfLiteraturePDFManuals_and_Quick_Guides/webroot/web/images/general/icons/icon_pdf.gifNoInstruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C4110109unosphere, LIT4110109, ion exchange, bulletin 4110109, uno2204Effective Removal of Negatively Charged Interfering Molecules From Proteins, Rev B2204/webroot/web/pdf/lsr/literature/Bulletin_2204B.pdfLiteraturePDFArticles_and_Whitepapers/webroot/web/images/general/icons/icon_pdf.gifNoEffective Removal of Negatively Charged Interfering Molecules From Proteins, Rev B2204bulletin 2204, ion exchange, lit2204, lipopolysaccharide, process scale, protein purification,
pyrogenic, anion, endotoxin, chromatography, column, columns, medium, media, resin,
resins, ion, exchange, iex, q, anion, anex, macroprep, macro prep, macro-prep, 1580040,
1560040, 1560041, 1560042, 1560043
1985Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange
Chromatography, Rev B.
1985/webroot/web/pdf/lsr/literature/Bulletin_1985.pdfLiteraturePDFArticles_and_Whitepapers/webroot/web/images/general/icons/icon_pdf.gifNoA Poster Presentation1985bulletin 1985, lit1985, chromatography, column, columns, medium, media, resin, resins,
protein, purification, monoclonal, antibody, virus, ion, exchange, iex, anion, cation,
anex, catex, cex, macro-prep
5644Macro-Prep® High Q Support Bulletin, Rev A5644/webroot/web/pdf/lsr/literature/Bullentin_5644A.pdfLiteraturePDFBrochures_and_Specifications/webroot/web/images/general/icons/icon_pdf.gifMacro-Prep® High Q Support Bulletin, Rev ANoMacro-Prep® High Q Support Bulletin, Rev A5644156-0043, proteins, 1560040, 158-0040, 1580040, binding, 156-0040, 1560043, 156-0042,
anion, anex, exchanger, resin, protein purification, rapid purifications, 1560041,
156-0041, 1560042, ion exchange, chromatography, 156-0043, 158-0040EDU, 1580040EDU
22922292Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A/webroot/web/pdf/lsr/literature/Bulletin_2292.pdfLiteraturePDFProduct Information Sheets/webroot/web/images/general/icons/icon_pdf.gifNoMacro-Prep Ion Exchange Supports Product Information Sheet, Rev ALife Science2292bulletin 2292, anion, cation, scale-up, LIT2292, process scaleLIT271LIT271Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D/webroot/web/pdf/lsr/literature/LIT271D.PDFLiteraturePDFManuals and Quick Guides/webroot/web/images/general/icons/icon_pdf.gifNoInstruction Manual, Macro-Prep Ion Exchange Supports, Rev DLife ScienceLIT271anion, bulletin LIT271, cation, scale-up, LIT271, process scale25242524Purification of Horse IgG<sub>T</sub> Using Macro-Prep DEAE and CHT Ceramic Hydroxyapatite
Type I Supports, Rev C
/webroot/web/pdf/lsr/literature/Bulletin_2524C.pdfLiteraturePDFApplication Notes/webroot/web/images/general/icons/icon_pdf.gifNoPurification of Horse IgG<sub>T</sub> Using Macro-Prep DEAE and CHT Ceramic Hydroxyapatite
Type I Supports, Rev C
Life Science2524immunoglobulins, Bio-Scale, chromatographic, columns, column, LIT2524, bulletin 2524,
Igg, immunoglobulin
2026Evaluation of Different Approaches for the Chromatographic Purification of Monoclonal
Antibodies
2026/webroot/web/pdf/lsr/literature/Bulletin_2026.pdfLiteraturePDFApplication_Notes/webroot/web/images/general/icons/icon_pdf.gifNoEvaluation of Different Approaches for the Chromatographic Purification of Monoclonal
Antibodies
2026bulletin 2026, lit2026, chromatography, column, columns, medium, media, resin, resins,
protein, purification, monoclonal, antibody, affinity, ion, exchange, iex, anion,
cation, anex, catex, cex
1942Macro-Prep DEAE Support for Chromatography of Biomolecules, Rev C.1942/webroot/web/pdf/lsr/literature/Bulletin_1942.pdfLiteraturePDFApplication_Notes/webroot/web/images/general/icons/icon_pdf.gifNoMacro-Prep DEAE Support for Chromatography of Biomolecules, Rev C.1942supports, separation, medium, protein purification, anion exchange, anion, media,
156-0020, 156-0021, 156-0022, 156-0023, 158-0020, 1560020, 1560021, 1560022, 1560023,
1580020, chromatography, column, columns, medium, media, resin, resins, protein, purification,
ion, exchange, iex, anion, cation, anex, catex, cex, macro-prep, macroprep, macro
prep, scale-up, snake venom, bulletin 1913, protein purification, process scale
LIT2714006129Instruction Manual, Macro-Prep 25 Ion Exchange Resins, Ver C4006129/webroot/web/pdf/lsr/literature/4006129.pdfLiteraturePDFManuals_and_Quick_Guides/webroot/web/images/general/icons/icon_pdf.gifInstruction Manual, Macro-Prep 25 Ion Exchange Resins, Ver CNoInstruction Manual, Macro-Prep 25 Ion Exchange Resins, Ver C4006129cation exchange, scale-up, 25q, 25s, anion, cation, macroprep, anion exchange, bulletin
4006129, LIT4006129, process scale
1801Protein and Peptide Purification Applications, Rev B1801/webroot/web/pdf/lsr/literature/Bulletin_1801.pdfLiteraturePDFApplication_Notes/webroot/web/images/general/icons/icon_pdf.gifNoProtein and Peptide Purification Applications, Rev B1801lit1801, bulletin 1801, chromatography, column, columns, medium, media, resin, resins,
protein, purification, affinity, profinity, profinia, ion, exchange, iex, anion, cation,
anex, catex, cex, mixed mode, mixed-mode, dual, multimode, multimodal, cht, cft, hydroxyapatite,
fluoroapatite, hic, hydrophobic, size exclusion, sec, gpc, gel permeation, desalting,
applications
LIT212Instruction Manual, AG® 1, AG MP-1, and AG 2 Strong Anion Exchange Resin, Rev CLIT212/webroot/web/pdf/lsr/literature/LIT212.pdfLiteraturePDFManuals_and_Quick_Guides/webroot/web/images/general/icons/icon_pdf.gifNoInstruction Manual, AG® 1, AG MP-1, and AG 2 Strong Anion Exchange Resin, Rev CLIT212lit212, ion exchange, anion, anex, ion exchange, chromatography, column, columns,
media, resin, resins, bulletin lit212, 140-1231, 140-1241, 140-1251, 140-1253, 140-1331,
140-1341, 140-1351, 143-1255, 143-1345, 1401231, 1401241, 1401251, 1401253, 1401331,
1401341, 1401351, 1431255, 1431345
Karen MossIon Exchange ChromatographyIon exchange chromatography is a technique for separating compounds based on their
net charge. Ion exchange chromatography media contain negatively or positively charged
functional groups covalently bound to a solid support, yielding either a cation or
anion exchanger.
separation, chromatography, ion exchange chromatography12/29/11 05:46 PM12/29/21 06:00 PMAE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VNenLSR/LSR/Technologies/ChromatographyN00Introduction to Chromatography
/en-us/applications-technologies/ion-exchange-chromatography?ID=LUSMIS7OP
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