Affi-Gel^® 10 and Affi-Gel^® 15 Resins

Overview

Affinity Chromatography Resin Selection Guide

	Matrix	Functional Group	Specificity	Capacity	Working pH	Pressure Limit	Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC	High capacity, pressure-stable polymer based on UNOsphere™ beads	NTA charged with Ni²⁺	Histidine	≥40 mg/ml*	2–14	45 psi (3.1 bar)	Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC	Pressure-stable polymer based on UNOsphere beads	IDA, available charged with Ni²⁺ and uncharged	Histidine	≥15 mg/ml*	1–14	100 psi (6.8 bar)	Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST**	Pressure-stable polymer based on UNOsphere beads	Immobilized glutathione	GST-tagged proteins	≥10 mg/ml	1–14	45 psi (3.1 bar)	Purification of recombinant GST-tagged proteins
Profinity eXact™***	Crosslinked 6% agarose	Subtilisin protease	Subtilisin prodomain	≥3 mg/ml tag-free protein	2–10	10 psi (minus system pressure)	Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel^® Protein A	Crosslinked agarose	Protein A 2 mg/ml	IgG		2–10	15 psi (1 bar)	Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep^® Protein A	Pressure-stable polymer	Protein A 2 mg/ml	IgG		2–10	1,000 psi (70 bar)	Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue	Crosslinked agarose	Cibacron Blue F3GA 1.9 mg/ml	Albumin; general	≥11 mg/ml	2–10	15 psi (1 bar)	Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue	Crosslinked agarose	Cibacron Blue F3GA and DEAE	Albumin and serum proteins	0.14 ml serum/ml gel	2–10	15 psi (1 bar)	Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue	Crosslinked agarose	Cibacron Blue F3GA and CM	Albumin and serum proteins	0.17–0.5 ml serum/ml gel	2–11	15 psi (1 bar)	Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin	Pressure-stable polymer	Polymyxin 2–4 mg/ml	Endotoxins	>5 mg/ml	2–10	1,000 psi (70 bar)	Endotoxin removal
Affi-Gel Boronate	Polyacrylamide gel	Boronate 1.05 ± 0.15	cis-diols meq/g	130 µmol sorbitol/ml	2–10	15 psi (1 bar)	Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide	Pressure-stable polymer based on UNOsphere beads	Epoxy group	Nucleophiles; amini, thiol, -COOH	36–40 mg/ml lgG	1–14	Up to 80 psi (5.5 bar)	Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10	Crosslinked agarose	N-hydroxy-succinimide ≥10 µmol/ml	-NH₂	35 mg/ml	3–11	15 psi (1 bar)	For coupling proteins with pI 6.5–11
Affi-Gel 15	Crosslinked agarose	N-hydroxy-succinimide ≥9 µmol/ml	-NH₂	35 mg/ml	3–11	15 psi (1 bar)	For coupling proteins with pI 6.5–11
Affi-Gel Hz	Crosslinked agarose	Hydrazide	Oxidized carbohydrates	1–5 mg/ml	2–10	15 psi (1 bar)	Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102	Crosslinked agarose	-NH₂ 16 ± 4 meq/ml	-COOH	40 mg	2–11	15 psi (1 bar)	Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

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Affi-Gel 10 and Affi-Gel 15 gels are activated affinity resins that provide spontaneous, rapid, and highly efficient coupling of ligands via primary amines.

Affi-Gel 10 medium is most efficient for coupling neutral or basic proteins with pI from 6.5 to 11
Affi-Gel 15 medium is recommended for coupling acidic proteins with pI <6.5

Affi-Gel 10 and Affi-Gel 15 resin offer:

Aqueous or anhydrous coupling conditions
Complete protein coupling within 4 hours at 4°C
Protein coupling capacity up to 35 mg/ml gel

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Affi-Gel 10 Gel

1536099
25 ml, activated affinity chromatography media, for basic (pI 6.5–11) proteins

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Affi-Gel 10 Gel

1536046
4 x 25 ml, activated affinity chromatography media, for basic (pI 6.5–11) proteins

1 1536046 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6046_0921120656303.xml 1536046 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1536046-affi-gel-10-gel 1536046 Affi-Gel 10 Gel Affi-Gel 10 Gel 4 x 25 ml, activated affinity chromatography media, for basic (pI 6.5–11) proteins 4 x 25 ml, activated affinity chromatography media, for basic (pI 6.5–11) proteins 4 x 25 ml, activated affinity chromatography media, for basic (pI 6.5–11) proteins /webroot/web/images/lsr/products/chromatography/sku_thumb/global/153-6046_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/153-6046_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/153-6046_feature.jpg RepEx 153-6046 4 x 25 ml, activated affinity chromatography media, for basic (pI 6.5–11) proteins 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536046&CartQuote=QT&quantity= true true false $593.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536046&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536046&quantity= 1536046

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Affi-Gel 10 Gel

153-1000
1 L, activated affinity chromatography media, for basic (pI 6.5–11) proteins

2 1531000 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-1000_0921120656383.xml 1531000 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1531000-affi-gel-10-gel 1531000 Affi-Gel 10 Gel Affi-Gel 10 Gel 1 L, activated affinity chromatography media, for basic (pI 6.5–11) proteins 1 L, activated affinity chromatography media, for basic (pI 6.5–11) proteins 1 L, activated affinity chromatography media, for basic (pI 6.5–11) proteins /webroot/web/images/ps/products/affinity_media/sku_thumb/global/153-1000_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/153-1000_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/153-1000_feature.jpg RepEx 153-1000 1 L, activated affinity chromatography media, for basic (pI 6.5–11) proteins 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1531000&CartQuote=QT&quantity= true true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1531000&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1531000&quantity= 153-1000

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Affi-Gel 15 Gel

1536051
25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins

3 1536051 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6051_0921120656408.xml 1536051 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1536051-affi-gel-15-gel 1536051 Affi-Gel 15 Gel Affi-Gel 15 Gel 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins /webroot/web/images/ps/products/affinity_media/sku_thumb/global/153-6051_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/153-6051_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/153-6051_feature.jpg RepEx 153-6051 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536051&CartQuote=QT&quantity= true true false $210.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536051&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536051&quantity= 1536051

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Affi-Gel 15 Gel

1536052
4 x 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins

4 1536052 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6052_0921120656359.xml 1536052 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1536052-affi-gel-15-gel 1536052 Affi-Gel 15 Gel Affi-Gel 15 Gel 4 x 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins 4 x 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins 4 x 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins /webroot/web/images/lsr/products/chromatography/sku_thumb/global/153-6052_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/153-6052_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/153-6052_feature.jpg RepEx 153-6052 4 x 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536052&CartQuote=QT&quantity= true true false $667.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536052&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536052&quantity= 1536052

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(Discontinued) Affi-Gel 15 Gel

153-1500
1 L, activated affinity chromatography media, for acidic (pI <6.5) proteins

This product is no longer available. Please contact technical support for more information on replacement product.

Affi-Gel 10/15 Combination

1536098
Pkg of 1, activated affinity media set, includes 2 x 25 ml Affi-Gel 10 gel and 2 x 25 ml Affi-Gel 15 gel

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Number	Description	Options
LIT156	Instruction Manual, Affi-Gel 10, Affi-Gel 15 Activated Immunoaffinity Supports, Rev B	Click to download
1085	Affi-Gel 10 and 15 Activated Affinity Media, Rev E	Click to download
5925	Affinity Purification Buffer Kit Product Information Sheet, Rev B	Click to download	5925 [ Add to Cart (Free) ]

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Affi-Gel^® 10 and Affi-Gel^® 15 Resins

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I cannot find the Certificate of Analysis I am looking for. Help!

Where can I find the catalog number, SKU number, or product number?

Where can I find the lot or control number?

Why are Certificates of Analysis not on the Documents tab?

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

Can I get a Certificate of Analysis even if my item is expired?

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

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Affi-Gel® 10 and Affi-Gel® 15 Resins

Overview

Affi-Gel 10 Gel

Affi-Gel 10 Gel

Affi-Gel 10 Gel

Affi-Gel 15 Gel

Affi-Gel 15 Gel

(Discontinued) Affi-Gel 15 Gel

Affi-Gel 10/15 Combination

I cannot find the Certificate of Analysis I am looking for. Help!

Where can I find the catalog number, SKU number, or product number?

Where can I find the lot or control number?

Why are Certificates of Analysis not on the Documents tab?

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

Can I get a Certificate of Analysis even if my item is expired?

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

Why does it say "Not Available" for a Certificate of Analysis listed in the search results?

Support

Ordering

Our Products

Corporate

Asia Pacific

Affi-Gel^® 10 and Affi-Gel^® 15 Resins