Affi-Gel 15 Gel

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Affi-Gel 15 Gel

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Affinity Resin Selection Guide
Affinity Chromatography Resin Selection Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
Nuvia™ IMAC High capacity, pressure-stable polymer based on UNOsphere™ beads NTA charged with Ni2+ Histidine ≥40 mg/ml* 2–14 45 psi
(3.1 bar)		 Purification of recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity™ IMAC Pressure-stable polymer based on UNOsphere beads IDA, available charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)		 Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals
Profinity GST** Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)		 Purification of recombinant GST-tagged proteins
Profinity eXact™*** Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi (minus system pressure) Generation of native, tag-free proteins by on-column purification and cleavage
Affi-Gel® Protein A Crosslinked agarose Protein A
2 mg/ml		 IgG   2–10 15 psi
(1 bar)		 Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible
Affi-Prep® Protein A Pressure-stable polymer Protein A
2 mg/ml		 IgG   2–10 1,000 psi (70 bar) Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)		 Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)		 Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml gel 2–11 15 psi
(1 bar)		 Produces albumin- and protease-free antibody preparation from serum without prior dialysis
Affi-Prep Polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)		 Endotoxin removal
Affi-Gel Boronate Polyacrylamide gel Boronate 1.05 ± 0.15 cis-diols

meq/g		 130 µmol sorbitol/ml 2–10 15 psi
(1 bar)		 Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity Epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles; amini, thiol, -COOH 36–40 mg/ml lgG 1–14 Up to 80 psi
(5.5 bar)		 Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)		 For coupling proteins with pI 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)		 For coupling proteins with pI 6.5–11
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)		 Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2 16 ± 4 meq/ml -COOH 40 mg 2–11 15 psi
(1 bar)		 Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.

Affi-Gel 10 and Affi-Gel 15 activated affinity media provide spontaneous, rapid, and highly efficient coupling of ligands via primary amines.
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Description

Affi-Gel 10 and Affi-Gel 15 activated affinity media provide spontaneous, rapid, and highly efficient coupling of ligands via primary amines.

  • Affi-Gel 10 support is most efficient for coupling neutral or basic proteins with pI from 6.5 to 11
  • Affi-Gel 15 gel is recommended for coupling acidic proteins with pI <6.5

Affi-Gel 10 and 15 media offer:

  • Spontaneous coupling under aqueous or anhydrous conditions
  • Complete protein coupling within 4 hours at 4°C
  • Protein coupling capacity up to 35 mg/ml gel

For more information, request Bulletin 1085.

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Affi-Gel 15 Gel

1536051
25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins

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Affi-Gel 15 Gel

1536052
4 x 25 ml, activated affinity chromatography media, for acidic (pI <6.5) proteins

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(Discontinued) Affi-Gel 15 Gel

153-1500
1 L, activated affinity chromatography media, for acidic (pI <6.5) proteins

This product is no longer available. Please contact technical support for more information on replacement product.
Affi-Gel 10/15 Combination

1536098
Pkg of 1, activated affinity media set, includes 2 x 25 ml Affi-Gel 10 gel and 2 x 25 ml Affi-Gel 15 gel

3 1536098 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6098_0921120656428.xml 1536098 /default/main/bio-rad/WORKAREA/default 09/21/09 12:06 PM en_US jthvldor 2017-11-14 07:08:02 true true internet/product_SKU jthvldor templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6098_0921120656428.xml LSR 2019-05-04 20:56:03 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555998735455 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 20:56:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6098_0921120656428.xml templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-6098_0921120656428.xml 1536098-affi-gel-10-15-combination 1536098 Affi-Gel 10/15 Combination Affi-Gel 10/15 Combination Pkg of 1, activated affinity media set, includes 2 x 25 ml Affi-Gel 10 gel and 2 x 25 ml Affi-Gel 15 gel Pkg of 1, activated affinity media set, includes 2 x 25 ml Affi-Gel 10 gel and 2 x 25 ml Affi-Gel 15 gel Pkg of 1, activated affinity media set, includes 2 x 25 ml Affi-Gel 10 gel and 2 x 25 ml Affi-Gel 15 gel /webroot/web/images/ps/products/affinity_media/sku_thumb/global/153-6098_tn.jpg /webroot/web/images/ps/products/affinity_media/sku_view/global/153-6098_view.jpg /webroot/web/images/ps/products/affinity_media/sku_feature/global/153-6098_feature.jpg RepEx 153-6098 Pkg of 1, activated affinity media set, includes 2 x 25 ml Affi-Gel 10 gel and 2 x 25 ml Affi-Gel 15 gel 09/21/09 12:06 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Chromatography/Chromatography-Media N 0 false $711.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536098&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536098&CartQuote=QT&quantity= 1251 true true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1536098&AddToDefaultWishList=true&quantity= 41105300 711.00 Product detail page 1536098
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Documents

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LIT156 Instruction Manual, Affi-Gel 10, Affi-Gel 15 Activated Immunoaffinity Supports, Rev B Click to download
1085 Affi-Gel 10 and 15 Activated Affinity Media, Rev E Click to download

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