
Overview
Matrix | Functional Group | Specificity | Capacity | Working pH | Pressure Limit | Applications | |
Ready-to-Use Affinity Media | |||||||
Nuvia™ IMAC | High capacity, pressure-stable polymer based on UNOsphere™ beads | NTA charged with Ni2+ | Histidine | ≥40 mg/ml* | 2–14 | 45 psi (3.1 bar) |
Purification of recombinant histidine-tagged proteins; can be charged with other transition metals |
Profinity™ IMAC | Pressure-stable polymer based on UNOsphere beads | IDA, available charged with Ni2+ and uncharged | Histidine | ≥15 mg/ml* | 1–14 | 100 psi (6.8 bar) |
Suitable for purification of larger and difficult-to-purify recombinant histidine-tagged proteins; can be charged with other transition metals |
Profinity GST** | Pressure-stable polymer based on UNOsphere beads | Immobilized glutathione | GST-tagged proteins | ≥10 mg/ml | 1–14 | 45 psi (3.1 bar) |
Purification of recombinant GST-tagged proteins |
Profinity eXact™*** | Crosslinked 6% agarose | Subtilisin protease | Subtilisin prodomain | ≥3 mg/ml tag-free protein | 2–10 | 10 psi (minus system pressure) | Generation of native, tag-free proteins by on-column purification and cleavage |
Affi-Gel® Protein A | Crosslinked agarose | Protein A 2 mg/ml |
IgG | 2–10 | 15 psi (1 bar) |
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG per ml of gel is possible | |
Affi-Prep® Protein A | Pressure-stable polymer | Protein A 2 mg/ml |
IgG | 2–10 | 1,000 psi (70 bar) | Purified IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications | |
Affi-Gel Blue | Crosslinked agarose | Cibacron Blue F3GA 1.9 mg/ml | Albumin; general | ≥11 mg/ml | 2–10 | 15 psi (1 bar) |
Binds many nucleotide-requiring enzymes, albumin, and other proteins |
DEAE Affi-Gel Blue | Crosslinked agarose | Cibacron Blue F3GA and DEAE | Albumin and serum proteins | 0.14 ml serum/ml gel | 2–10 | 15 psi (1 bar) |
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation |
CM Affi-Gel Blue | Crosslinked agarose | Cibacron Blue F3GA and CM | Albumin and serum proteins | 0.17–0.5 ml serum/ml gel | 2–11 | 15 psi (1 bar) |
Produces albumin- and protease-free antibody preparation from serum without prior dialysis |
Affi-Prep Polymyxin | Pressure-stable polymer | Polymyxin 2–4 mg/ml | Endotoxins | >5 mg/ml | 2–10 | 1,000 psi (70 bar) |
Endotoxin removal |
Affi-Gel Boronate | Polyacrylamide gel | Boronate 1.05 ± 0.15 | cis-diols meq/g |
130 µmol sorbitol/ml | 2–10 | 15 psi (1 bar) |
Adsorption of cis-hydroxyl–containing molecules, including sugars, nucleotides, and glycopeptides |
Activated Media for Spontaneous Ligand Immobilization | |||||||
Profinity Epoxide | Pressure-stable polymer based on UNOsphere beads | Epoxy group | Nucleophiles; amini, thiol, -COOH | 36–40 mg/ml lgG | 1–14 | Up to 80 psi (5.5 bar) |
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins) |
Affi-Gel 10 | Crosslinked agarose | N-hydroxy-succinimide ≥10 µmol/ml | -NH2 | 35 mg/ml | 3–11 | 15 psi (1 bar) |
For coupling proteins with pI 6.5–11 |
Affi-Gel 15 | Crosslinked agarose | N-hydroxy-succinimide ≥9 µmol/ml | -NH2 | 35 mg/ml | 3–11 | 15 psi (1 bar) |
For coupling proteins with pI 6.5–11 |
Affi-Gel Hz | Crosslinked agarose | Hydrazide | Oxidized carbohydrates | 1–5 mg/ml | 2–10 | 15 psi (1 bar) |
Immobilization of immunoglobulins and other glycoproteins via carbohydrate molecules |
Affinity Media Using Carbodiimide Activation | |||||||
Affi-Gel 102 | Crosslinked agarose | -NH2 16 ± 4 meq/ml | -COOH | 40 mg | 2–11 | 15 psi (1 bar) |
Carbodiimide coupling of carboxyl-containing ligand |
* Refer to Bulletin 3193 for purification conditions.
** For example, Profinity GST Resin is available only in prepacked cartridges.
***Profinity eXact Purification Resin.
Affi-Gel 10 and Affi-Gel 15 activated affinity media provide spontaneous, rapid, and highly efficient coupling of ligands via primary amines.
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