SsoAdvanced Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml) #1725282

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1725282 [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [18.204.42.98, 10.232.19.238] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/sku/1725282-ssoadvanced-universal-probes-supermix-1000-x-20-ul-rxns-10-ml-10-x-1-ml] [x-amzn-trace-id] = [Root=1-600bff50-392e6c1120b95776751b2e7e] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=1725282] [host] = [10.232.0.21:1776] [x-request-uri] = [/en-us/sku/1725282-ssoadvanced-universal-probes-supermix-1000-x-20-ul-rxns-10-ml-10-x-1-ml] [connection] = [Keep-Alive] [accept-encoding] = [br,gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductSKU pageStyleKey 1725282-ssoadvanced-universal-probes-supermix-1000-x-20-ul-rxns-10-ml-10-x-1-ml LSR en US en-us /prd false 07/30/13 03:25 AM en_US true internet/generic 2020-09-18 04:55:37 /default/main/bio-rad/STAGING 1375179935592 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml page_EDUPriceOverlay /default/main/bio-rad/WORKAREA/default jddgmm0t 2017-11-14 06:29:46 true jddgmm0t templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml LSE N /default/main/bio-rad en 07/30/37 05:00 PM 1579275613146 2020-09-18 04:55:37 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/LSE/page_EDUPriceOverlay_1375183846_XX.xml edu-price-overlay 1 1375179935592 page_EDUPriceOverlay <div id="eduPriceOverlay" class="overlay" style="display: block;"> <p class="headerClassText">What is the education discount policy?</p> <p class="descClassText">For more than 20 years, Bio-Rad has made science education a major priority. 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This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/pcr-inhibitor-tolerance-tutor-noggin.html');">Sso7d</a>, stabilizes the polymerase-template complex, providing <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html');">superior inhibitor tolerance</a>, increased <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_detail/description/global/processivity.html');">processivity</a>, specificity, and greater speed without affecting PCR <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html');">sensitivity</a>, efficiency, or reproducibility.</p> <p>SsoAdvanced universal probes supermix lets you:</p> <ul> <li><strong>Carry out high-performance singleplex and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/duplex-reaction.html');">duplex</a> reactions</strong> &mdash; the Sso7d fusion polymerase and advanced formulation enable <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/long-amplicon-gc-rich-duplex-reaction.html');">robust performance</a> in singleplex or duplex real-time PCR reactions, providing the highest data precision and allowing cost and time savings when combining 2 assays in a single well</li> <li><strong>Use any real-time PCR system</strong> &mdash; the universal reference dye in this supermix enables ROX normalization of quantitative PCR (qPCR) data regardless of the ROX level requirements of the qPCR system</li> <li><strong>Achieve superior real-time PCR results under various conditions</strong> &mdash; our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges</li> <li><strong>Increase your qPCR sensitivity and efficiency of detection from compromised samples</strong> &mdash; the Sso7d fusion polymerase has <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html');">increased resistance</a> to a wide variety of PCR inhibitors, which helps provide <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html');">better sensitivity</a> and overall performance</li> <li><strong>Decrease time to results without compromising qPCR data quality</strong> &mdash; the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>SsoAdvanced Universal Probes Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Applications and Uses of SsoAdvanced Universal Probes Supermix</h2> <ul> <li><a href="/evportal/destination/solutions?catID=LUSO4W8UU">qPCR/real-time PCR</a></li> <li><a href="/evportal/destination/solutions?catID=LUSNINKSY">Gene expression analysis</a></li> <li>Absolute quantification</li> <li>Genotyping (allelic discrimination)</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Characterization of genetically modified organisms (GMO)</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td><strong>Number of 20 &mu;l Reactions</strong></td> <td><strong>Total Volume</strong></td> <td><strong>Number of Vials</strong></td> <td><strong>Catalog #</strong></td> </tr> <tr class="pd_colorbackground"> <td>200</td> <td>2 ml</td> <td>2 x 1 ml</td> <td><a href="/evportal/destination/commerce/sku_detail?productID=172-5280">1725280</a></td> </tr> <tr> <td>500</td> <td>5 ml</td> <td>5 x 1 ml</td> <td><a href="/evportal/destination/commerce/sku_detail?productID=172-5281">1725281</a></td> </tr> <tr class="pd_colorbackground"> <td>1,000</td> <td>10 ml</td> <td>10 x 1 ml</td> <td>1725282</td> </tr> <tr> <td>2,500</td> <td>25 ml</td> <td>5 x 5 ml</td> <td><a href="/evportal/destination/commerce/sku_detail?productID=172-5284">1725284</a></td> </tr> <tr class="pd_colorbackground"> <td>5,000</td> <td>50 ml</td> <td>10 x 5 ml</td> <td><a href="/evportal/destination/commerce/sku_detail?productID=172-5285">1725285</a></td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <p>We have developed <a href="/evportal/destination/commerce/product_detail?catID=MH5H1EE8Z">SsoAdvanced Universal SYBR<sup>&reg;</sup> Green Supermix</a>, which is available in several formats (200&ndash;5,000 reactions).</p> <p>Bio-Rad offers a complete workflow solution for real-time PCR, including:</p> <ul> <li><a href="/en-us/category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005a">RNA isolation kits</a></li> <li><a href="/en-us/category/cdna-synthesis-kits?ID=M87EPGE8Z">Reverse transcription kits</a></li> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents?ID=M87EKA15">Real-time PCR supermixes</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/evportal/destination/solutions?catID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into <a href="/evportal/destination/solutions?catID=LUSOA9LPT">analysis of qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/bulletin-10031340.pdf">troubleshooting reactions</a>.</p> <p class="caption">* U.S. patents 6,627,424; 7,541,170; and 7,560,260.</p> /webroot/web/html/lsr/products/SKUs 172-5282.html /webroot/web/html/lsr/products/SKUs/172-5282.html Product expiration (after shipping date) 2026 12 months 1 0 Storage at 4°C (after thawing) 2806 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false $771.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725282&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725282&CartQuote=QT&quantity= 1459 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725282&AddToDefaultWishList=true&quantity= 41106300 771.00 SKU detail page 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10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, ROX normalization dyes, for 1,000 x 20 µl reactions

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To support this effort, the company has implemented a discount policy that allows K&ndash;12 and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. These discounted products are indicated by an EDU suffix (for example, 1660003EDU).</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the K&ndash;12 or college level, contact your local <a href="/en-au/contact-us">Customer Service</a> office to establish an education account number.</p> </div> 0 /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/37 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE N 0 {"ecommerce":{"detail":{"products":{"price":"$771.00","name":"SsoAdvanced Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml)","variant":"BRC","id":"1725282","category":"BRC","brand":"BRC"}}},"event":"detail"} 1725282 02/28/13 05:07 PM en_US true internet/product_SKU 2020-12-09 20:52:07 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5282_1362100340.xml /default/main/bio-rad/WORKAREA/default jcrucyrm 2017-11-14 07:07:37 true jcrucyrm templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5282_1362100340.xml LSR N /default/main/bio-rad en 02/28/23 05:11 PM 1607421453854 2020-12-09 20:52:07 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5282_1362100340.xml 1725282-ssoadvanced-universal-probes-supermix-1000-x-20-ul-rxns-10-ml-10-x-1-ml 1 1725282 SsoAdvanced Universal Probes Supermix SsoAdvanced Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml) 10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, stabilizers, ROX normalization dyes, for 1,000 x 20 &micro;l reactions 10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, stabilizers, ROX normalization dyes, for 1,000 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/ssoadvanced-universal-probes-supermix-172-5282-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/ssoadvanced-universal-probes-supermix-172-5282-view.jpg SsoAdvanced Universal Probes Supermix, 1,000 x 20 µl rxns, 10 ml (10 x 1 ml) Molly Gregg SsoAdvanced Universal Probes Supermix 10 ml (10 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl<sub>2</sub>, stabilizers, ROX normalization dyes, for 1,000 x 20 &micro;l reactions 172-5282, 1725282, ssoadvanced, universal, probes, supermix, super, master, mix, mastermix, detection, amplification, real-time, pcr, real, time, qpcr, sso7d, polymerase, rox 02/28/13 05:07 PM 02/28/23 05:11 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>SsoAdvanced Universal Probes Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/pcr-inhibitor-tolerance-tutor-noggin.html');">Sso7d</a>, stabilizes the polymerase-template complex, providing <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html');">superior inhibitor tolerance</a>, increased <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_detail/description/global/processivity.html');">processivity</a>, specificity, and greater speed without affecting PCR <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html');">sensitivity</a>, efficiency, or reproducibility.</p> <p>SsoAdvanced universal probes supermix lets you:</p> <ul> <li><strong>Carry out high-performance singleplex and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/duplex-reaction.html');">duplex</a> reactions</strong> &mdash; the Sso7d fusion polymerase and advanced formulation enable <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/long-amplicon-gc-rich-duplex-reaction.html');">robust performance</a> in singleplex or duplex real-time PCR reactions, providing the highest data precision and allowing cost and time savings when combining 2 assays in a single well</li> <li><strong>Use any real-time PCR system</strong> &mdash; the universal reference dye in this supermix enables ROX normalization of quantitative PCR (qPCR) data regardless of the ROX level requirements of the qPCR system</li> <li><strong>Achieve superior real-time PCR results under various conditions</strong> &mdash; our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges</li> <li><strong>Increase your qPCR sensitivity and efficiency of detection from compromised samples</strong> &mdash; the Sso7d fusion polymerase has <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/category_overlay/global/ssoadv-pcr-inhibitor-tolerance.html');">increased resistance</a> to a wide variety of PCR inhibitors, which helps provide <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/single-copy-detection.html');">better sensitivity</a> and overall performance</li> <li><strong>Decrease time to results without compromising qPCR data quality</strong> &mdash; the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation</li> </ul> <h2 class="descHeader">Instrument Compatibility</h2> <p>SsoAdvanced Universal Probes Supermix is compatible with <a href="/en-us/category/real-time-pcr-detection-systems?ID=059db09c-88a4-44ad-99f8-78635d8d54db">Bio-Rad Real-Time PCR Detection Systems</a> and commercially available systems listed below:</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td colspan="2" valign="middle"><strong>Systems</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">Applied Biosystems</td> <td valign="top"> <ul> <li>StepOne/StepOnePlus</li> <li>7500, ViiA 7</li> <li>7000, 7300, 7700, 7900HT, QuantStudio Systems</li> </ul> </td> </tr> <tr> <td valign="top">Stratagene</td> <td valign="top"> <ul> <li>Mx3000P, 3005P, 4000</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Eppendorf</td> <td valign="top"> <ul> <li>Mastercycler ep realplex 2, 4</li> </ul> </td> </tr> <tr> <td valign="top">QIAGEN</td> <td valign="top"> <ul> <li>Corbett Rotor-Gene 3000, 6000, Q</li> </ul> </td> </tr> <tr class="pd_colorbackground"> <td valign="top">Roche</td> <td valign="top"> <ul> <li>LightCycler 480, 96</li> <li>LightCycler 1.0, 1.5, 2.0*</li> </ul> </td> </tr> <tr> <td valign="top">BioFire</td> <td valign="top"> <ul> <li>LightScanner HR-1</li> <li>LightScanner 32*</li> </ul> </td> </tr> </tbody> </table> <p class="caption caption-margin">* BSA must be added according to instrument specifications</p> <h2 class="descHeader">Applications and Uses of SsoAdvanced Universal Probes Supermix</h2> <ul> <li><a href="/evportal/destination/solutions?catID=LUSO4W8UU">qPCR/real-time PCR</a></li> <li><a href="/evportal/destination/solutions?catID=LUSNINKSY">Gene expression analysis</a></li> <li>Absolute quantification</li> <li>Genotyping (allelic discrimination)</li> <li>Mutation detection</li> <li>Pathogen detection</li> <li>Viral detection (load)</li> <li>Characterization of genetically modified organisms (GMO)</li> <li>Genetic profiling</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td><strong>Number of 20 &mu;l Reactions</strong></td> <td><strong>Total Volume</strong></td> <td><strong>Number of Vials</strong></td> <td><strong>Catalog #</strong></td> </tr> <tr class="pd_colorbackground"> <td>200</td> <td>2 ml</td> <td>2 x 1 ml</td> <td><a href="/evportal/destination/commerce/sku_detail?productID=172-5280">1725280</a></td> </tr> <tr> <td>500</td> <td>5 ml</td> <td>5 x 1 ml</td> <td><a href="/evportal/destination/commerce/sku_detail?productID=172-5281">1725281</a></td> </tr> <tr class="pd_colorbackground"> <td>1,000</td> <td>10 ml</td> <td>10 x 1 ml</td> <td>1725282</td> </tr> <tr> <td>2,500</td> <td>25 ml</td> <td>5 x 5 ml</td> <td><a href="/evportal/destination/commerce/sku_detail?productID=172-5284">1725284</a></td> </tr> <tr class="pd_colorbackground"> <td>5,000</td> <td>50 ml</td> <td>10 x 5 ml</td> <td><a href="/evportal/destination/commerce/sku_detail?productID=172-5285">1725285</a></td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <p>We have developed <a href="/evportal/destination/commerce/product_detail?catID=MH5H1EE8Z">SsoAdvanced Universal SYBR<sup>&reg;</sup> Green Supermix</a>, which is available in several formats (200&ndash;5,000 reactions).</p> <p>Bio-Rad offers a complete workflow solution for real-time PCR, including:</p> <ul> <li><a href="/en-us/category/rna-isolation?ID=2b86ef8e-07b5-4549-a544-0293620bb005a">RNA isolation kits</a></li> <li><a href="/en-us/category/cdna-synthesis-kits?ID=M87EPGE8Z">Reverse transcription kits</a></li> <li><a href="/en-us/category/pcr-reagents-qpcr-reagents?ID=M87EKA15">Real-time PCR supermixes</a></li> </ul> <h2 class="descHeader">More Information</h2> <p>To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step <a href="/evportal/destination/solutions?catID=LUSO7RIVK">design and optimization protocols</a>. We also provide insights into <a href="/evportal/destination/solutions?catID=LUSOA9LPT">analysis of qPCR data</a> and <a href="/webroot/web/pdf/lsr/literature/bulletin-10031340.pdf">troubleshooting reactions</a>.</p> <p class="caption">* U.S. patents 6,627,424; 7,541,170; and 7,560,260.</p> /webroot/web/html/lsr/products/SKUs 172-5282.html /webroot/web/html/lsr/products/SKUs/172-5282.html Product expiration (after shipping date) 2026 12 months 1 0 Storage at 4°C (after thawing) 2806 Up to 3 months 1 0 Customer guarantee (minimum usage time) at –20°C 2530 12 months 1 0 false $771.00 false $ null true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725282&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725282&CartQuote=QT&quantity= 1459 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725282&AddToDefaultWishList=true&quantity= 41106300 771.00 SKU detail page 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SsoAdvanced Universal Probes Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.

SsoAdvanced universal probes supermix lets you:

  • Carry out high-performance singleplex and duplex reactions — the Sso7d fusion polymerase and advanced formulation enable robust performance in singleplex or duplex real-time PCR reactions, providing the highest data precision and allowing cost and time savings when combining 2 assays in a single well
  • Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of quantitative PCR (qPCR) data regardless of the ROX level requirements of the qPCR system
  • Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
  • Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
  • Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation

Instrument Compatibility

SsoAdvanced Universal Probes Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses of SsoAdvanced Universal Probes Supermix

  • qPCR/real-time PCR
  • Gene expression analysis
  • Absolute quantification
  • Genotyping (allelic discrimination)
  • Mutation detection
  • Pathogen detection
  • Viral detection (load)
  • Characterization of genetically modified organisms (GMO)
  • Genetic profiling

Packaging Options

Number of 20 μl Reactions Total Volume Number of Vials Catalog #
200 2 ml 2 x 1 ml 1725280
500 5 ml 5 x 1 ml 1725281
1,000 10 ml 10 x 1 ml 1725282
2,500 25 ml 5 x 5 ml 1725284
5,000 50 ml 10 x 5 ml 1725285

Related Products

We have developed SsoAdvanced Universal SYBR® Green Supermix, which is available in several formats (200–5,000 reactions).

Bio-Rad offers a complete workflow solution for real-time PCR, including:

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

Product expiration (after shipping date) 12 months
Storage at 4°C (after thawing) Up to 3 months
Customer guarantee (minimum usage time) at –20°C 12 months
Number Description Options
Available Translation
SsoAdvanced Universal Probes Supermix  (SDS)gene

Bulletin# 172-5280-MSDS

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

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