SsoAdvanced™ Universal Inhibitor-Tolerant SYBR® Green Supermix, 500 x 20 µl rxns, 5 ml (1 x 5 ml) #1725017

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Overview

500 x 20 µl reactions, 5 ml (1 x 5 ml) 2x supermix, contains Sso7d fusion polymerase in an advanced buffer formulation
Safety Data Sheet

Safety Data Sheet

Description

Description

Use SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix for qPCR with crude samples and difficult-to-amplify target sequences. This 2x concentrated supermix features our patented* Sso7d fusion DNA polymerase and an advanced buffering chemistry optimized for a broad range of conditions on any real-time PCR instrument.

Specially formulated for high performance in difficult sample matrices:

  • Crude lysates from plants, seeds, bacteria, FFPE, and mammalian cells
  • Samples containing PCR inhibitors — heparin, hematin, polysaccharides, polyphenols, salts, humic acid, indigo, melanin, cell culture media, and others

Real-time PCR applications include:

  • Viral and bacterial detection
  • GMO testing
  • SNP genotyping
  • Mutation detection
  • Environmental monitoring
  • Gene expression
  • Pathway analysis

Instrument Compatibility

SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Packaging Options

  • 200 x 20 µl reactions (172-5016)
  • 500 x 20 µl reactions (172-5017)
  • 1,000 x 20 µl reactions (172-5018)

Related Products

Bio-Rad offers complete workflow solutions for real-time PCR:

* U.S. patents 6,627,424; 7,541,170; and 7,560,260

Specifications

Specifications

Product expiration (after shipping date)
12 months
Storage at 4°C (after thawing)
Up to 3 months
Customer guarantee (maximum usage time) at –20ºC
12 months