SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix

Earn a free one-year service contract on your CFX System with purchase of Reagents.

CFX Loyalty Program

SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix is an exclusive high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein polymerase technology and advanced buffer formulation. This supermix is specifically formulated for SYBR Green-based qPCR with challenging samples including crude lysates and inhibitor-rich samples. This supermix delivers higher processivity, unrivaled PCR inhibitor tolerance, and robust performance across a wide range of applications on any qPCR platform and is validated to ensure optimal results with Bio-Rad’s PrimePCR Gene Expression Assays.

SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix allows you to:

• Skip RNA and DNA extraction by using crude samples — achieve superior data using crude lysates from plants, seeds, cells, bacteria, or FFPE
• Power through PCR inhibitors — delivers unrivaled performance with common PCR inhibitors such as heparin, polysaccharides, culture media, and more
• Increase cDNA loading — add up to 20% cDNA from our iScript Advanced cDNA Synthesis Kit for RT-qPCR or iScript Reverse Transcription Supermix
• Obtain high-quality real-time PCR results under various experimental conditions — for example, primer concentrations, annealing temperatures, salt concentrations, and/or cycling protocol (standard or fast)
• Shorten time to results while maintaining qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
• Use with any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system

Instrument Compatibility

SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

* BSA must be added according to instrument specifications

Applications and Uses of SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix

• Viral and bacterial detection
• GMO testing
• SNP genotyping
• Mutation detection
• Environmental monitoring
• Gene expression
• Pathway analysis

Related Products

• SsoAdvanced Universal SYBR Green Supermix
• SsoAdvanced Universal Probes Supermix

Bio-Rad offers complete workflow solutions for real-time PCR:

• RNA Isolation Kits
• cDNA Synthesis Kits
• Real-Time PCR Supermixes
• PCR Plastic Consumables
• PrimePCR PCR Primers and Assays

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols. We also provide insights into analysis of qPCR data and troubleshooting reactions.

* U.S. patent 7,560,260