TransFectin™ Lipid Reagent, 0.5 ml #1703350

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0.5 ml, 1.5 mg/ml lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery

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Choose the general purpose TransFectin Lipid Reagent for high efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells. The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.

  • 0.5 ml is generally sufficient for 60 to 100 cell transfections in 35 mm plates
  • Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability
  • Transfection at culture densities from 40% to 90%
  • Designed to work in media containing serum but can be used in the absence of serum
  • No post transfection media changes required
  • Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene

The Transfection Protocol Online Library contains protocols from a range of sources for transfection using lipids, electroporation and biolistic particle delivery. The library has a comprehensive list of cell lines and primary cultures. Use the information in the library as a starting point for designing protocols for your cells. The TransFectin Lipid Reagent Instruction Manual provides information about optimization as well as changes in protocols when changing from a different cationic lipid.

Packaging Options

Volume Number of Vials Catalog Number
0.5 ml 1 x 0.5 ml 1703350
1 ml 1 x 1 ml 1703351
5 ml 5 x 1 ml 1703352

Related Products

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Supporting Documentation

Online Applications

Transfection

Available Translation
TransFectin Lipid Reagent  (SDS)gene

Bulletin# 170-3350-MSDS

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