Overview
1.0 ml, 1.5 mg/ml lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery
Safety Data Sheet
Description
Description
Choose the general purpose TransFectin Lipid Reagent for high efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells. The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity.
- 1 ml is generally sufficient for 125 to 200 cell transfections in 35 mm plates
- Simple 3-step protocol gives high-efficiency transfection in a broad range of cells with high levels of cell viability
- Transfection at culture densities from 40% to 90%
- Designed to work in media containing serum but can be used in the absence of serum
- No post transfection media changes required
- Polystyrene is recommended because cationic lipid-plasmid complexes may bind to polypropylene
The Transfection Protocol Online Library contains protocols from a range of sources for transfection using lipids, electroporation and biolistic particle delivery. The library has a comprehensive list of cell lines and primary cultures. Use the information in the library as a starting point for designing protocols for your cells. The TransFectin Lipid Reagent Instruction Manual provides information about optimization as well as changes in protocols when changing from a different cationic lipid.
Packaging Options
| Volume | Number of Vials | Catalog Number |
| 0.5 ml | 1 x 0.5 ml | 1703350 |
| 1 ml | 1 x 1 ml | 1703351 |
| 5 ml | 5 x 1 ml | 1703352 |
Related Products
- siLentFect™ Lipid Reagent for RNAi is optimized for delivery of siRNA alone, or cotransfected with a small reporter-plasmid
- SmartSpec™ Plus Spectrophotometer
- Fixable Viability Dyes
Related Categories
- Electroporation
- Biolistic Particle Delivery Systems
- Nucleic Acid Extraction & Purification
- PCR Amplification
Supporting Documentation
- TransFectin Lipid Reagent Brochure
- TransFectin Lipid Reagent Instruction Manual
- Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types Application Note
- Transfection Reagent References