Frequently Asked Questions
Learn more about how to use the SEQuoia RiboDepletion Kit.
SEQuoia RiboDepletion Kit is a stand-alone, post-library depletion kit that eliminate fragments derived from cytoplasmic ribosomal RNA (rRNA) and mitochondrial ribosomal RNA (mt rRNA) sequences from an RNA-Seq library. The ribosomal RNA is abundant, constituting 80–90% of total RNA. Efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples.
Depletion of rRNA from total RNA before library construction can result in the loss of rare or low-abundance transcripts. SEQuoia RiboDepletion Kit depletes cDNA library fragments that were derived from rRNA post-library preparation, exhibiting superior performance in profiling the whole transcriptome, retaining and capturing small RNAs while minimizing the loss of rare transcripts and RNA from limited or degraded samples. This stand-alone ribodepletion kit is optimized to work with a broad input range (0.1–20 ng of RNA-Seq library) and is compatible with most available library prep kits. With the innovative post-library depletion technology, multiple pre-indexed libraries can be pooled and depleted in a single reaction, maximizing the depletion efficiency and saving significant time and cost.
The streamline workflow can be finished within 2 hours.
A Novel Technology for Post-Library Depletion Strategy
Features and Benefits
- Effectively eliminate ribosomal and mitochondrial RNA fragments from an RNA-Seq library
- Retain rare transcripts commonly lost from pre-library RNA depletion strategy
- Pool barcoded libraries into one depletion reaction to save time and money
Each kit comes with two reagent boxes. Box A includes reagents for cDNA hybridization, depletion and amplification; store at
–20ºC. Box B includes purification beads; store at 4ºC.
SEQuoia RiboDepletion Kit FAQs
SEQuoia RiboDepletion Kit Technology
What is the benefit of depleting rRNA-derived fragments after library construction?
Why does a library with small insert size have higher percent of reads mapping to rRNA?
Does the SEQuoia RiboDepletion Kit deplete cytoplasmic and mitochondrial ribosomal RNA?
SEQuia RiboDepletion Kit Compatibility
What species are compatible with the SEQuoia RiboDepletion Kit?
Can I use the SEQuoia RiboDepletion Kit with other library prep kits?
Is this product compatible with degraded or fragmented RNA?
SEQuoia RiboDepletion Kit Usage
What is the total RNA input I should use?
What is the percentage of rRNA remains after depletion using the SEQuoia RiboDepletion Kit?
Can libraries constructed using different library preparation kits be pooled into a single reaction?
How many libraries can be pooled into a single depletion reaction when using the SEQuoia RiboDepletion Kit?
How can the percentage of residual rRNA be calculated using SeqSense NGS Data Analysis Software?
How do I calculate the percentage of residual rRNA using a different pipeline to process the FASTQ file?
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