SeqSense NGS Data Analysis Software

SEQuoia RiboDepletion Kit

Retain rare transcripts, and get the most from your sequencing
samples with the SEQuoia RiboDepletion Kit.

SeqSense NGS Data Analysis is a purpose-built workflow for analyzing and visualizing the next-generation sequencing (NGS) data produced using the SEQuoia Complete Stranded RNA Library Prep Kit.

SeqSense NGS Data Analysis Software Options

SeqSense Analysis is available in two options to work online, as a web application, or deployed locally, using your own infrastructure, through a Docker container. Both versions use FASTQ files as input for secondary analysis, using the same underlying pipeline, and can produce BAM files, count matrices, and reports as output for tertiary analysis.

 

SeqSense NGS Analysis Software User Guides

  • SeqSense Analysis Toolkit User Guide
    (494 KB)

    This tutorial illustrates how the SeqSense Analysis Toolkit is used with the SEQuoia Complete Stranded RNA Library Prep Kit, and provides the necessary information, scripts, and libraries to analyze the SeqSense Complete RNA data.

  • SeqSense Analysis Solution User Guide
    (691 KB)

    This tutorial illustrates how the SeqSense Analysis Solution is used with the SEQuoia Complete Stranded RNA Library Prep Kit to analyze the SeqSense Complete RNA data.

SEQuoia and SeqSense NGS Analysis Workflow

SeqSense NGS Data Analysis provides a bioinformatics pipeline uniquely optimized for mapping, aligning, counting, and normalizing reads generated from libraries containing both short and long RNA. Based on the STAR ultrafast universal RNA-seq aligner (Dobin 2013), the SeqSense RNA-Seq data analysis pipeline was designed to leverage the differentiated capabilities of the SEQuoia Complete Stranded RNA Library Prep Kit.

  • RNA-Seq Library Construction

    SEQuoia Complete Stranded RNA Library Prep Kit with Thermal Cycling

  • Sequencing

    Illumina NGS Platforms

  • Data Analysis Pipeline

    SeqSense Pipeline

  • Reporting & Visualization

    SeqSense Analysis Solutions & Toolkit

NGS Data Analysis Resources

  • The Power of SEQzyme

    Discover how SEQzyme, a proprietary, engineered retrotransposon efficiently captures all types and sizes of RNA species in a novel enzymatic reaction, significantly improving the diversity and quality of RNA libraries, even from limited or degraded RNA samples.

  • Revolutionizing Transcriptomics

    Learn how the new SEQuoia Complete Stranded RNA Library Prep Kit efficiently captures both long RNA and short RNAs less than 200 bp from all sample types, including limited or degraded RNA samples using a rapid, efficient, and sensitive workflow.

Citations

Dobin A, Davis CA, Schlesinger F, et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics. 2013;29(1):15–21. doi:10.1093/bioinformatics/bts635 PMCID: PMC3530905

Related Products

  • SEQuoia Complete Stranded RNA Library Prep Kit

    SEQuoia Complete Stranded RNA Library Prep Kit

    High-performance RNA-Seq kit that captures long and short RNAs in a single library.

  • SEQuoia RiboDepletion Kit

    Effectively eliminates ribosomal RNA and mitochondrial ribosomal RNA sequences from an RNA-Seq library.

  • Thermal Cyclers

    Thermal Cyclers for PCR

    Personal units and flexible modular thermal cyclers provide low- to high-throughput capabilities for all your PCR amplification needs

  • PCR Consumables

    PCR Plastic Consumables

    A large selection of thin-wall polypropylene PCR tubes, PCR plates, seals, and accessories precisely manufactured for various PCR applications

 

Single-Cell NGS Sample Preparation