The ReadyPrep protein extraction kit (soluble/insoluble) separates total protein samples into two fractions based on differential protein solubility. It generates a soluble fraction containing hydrophilic proteins and an insoluble fraction containing hydrophobic proteins. Proteins in the insoluble fraction are eventually resolubilized in ReadyPrep 2-D rehydration/solubilization buffer 1, a strongly chaotropic solution containing the zwitterionic detergent ASB-14. This simple fractionation strategy results in less complex samples and leads to improved identification of low-abundance proteins and a better overall view of the proteome.
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