Gel Doc EZ Gel Documentation System

Gel Doc EZ Gel Documentation System

The Gel Doc EZ System is a compact and automated gel imaging system. Trays specific for different applications afford flexibility, and the included Image Lab™ Software provides publication-quality images and analyzed results with a push of a but

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Overview

The Gel Doc EZ System is a compact and automated gel imaging instrument designed to yield publication-quality images and analyzed results with just the push of a button. The Gel Doc EZ System provides unparalleled flexibility with the use of four application-specific trays:

  • UV tray for ethidium bromide staining of DNA gels and fluorescence imaging
  • White tray for Coomassie, copper, silver, and zinc stains
  • Blue tray for nucleic acid applications with SYBR stains
  • Stain-free tray for stain-free imaging with Bio-Rad stain-free gels

The intuitive Image Lab Software bundled with the Gel Doc EZ Gel Imaging System includes capabilities such as auto image capture, auto analysis, user preferences, and many other features, making gel imaging and analysis incredibly easy.

Features and Benefits of the Gel Doc EZ System

  • Easy to use — researchers unfamiliar with the Gel Doc EZ imaging system can learn to use it quickly and easily because there is no need for manual control of filters, lens, or lights. Create a default protocol once, then use the green button on the front of the instrument to use these gel imaging and analysis settings time and again
  • Publication-quality images and full data analysis — get clean and smooth publication-ready images with decreased pixelation when images are cropped or zoomed. Get analyzed results including relative molecular weights, bands quantitation, Excel reports, PDFs, and more in a matter of minutes
  • Time and space savings — obtain results faster without spending time on various steps in image acquisition and save valuable bench space using this compact system
  • Modular design and flexible options — use specific trays for different gel imaging applications. Clearly defined and color-coded trays eliminate confusion. Purchase only what you need and upgrade as your research needs change
  • Stain-free technology — condense your 2 hr Coomassie protocol into a 5 min stain-and-image step with this stain-free gel imaging system. Bio-Rad stain-free gels are western blot compatible, allowing you to check electrophoresis results and quality prior to western blotting. These “green” gels provide equal or better sensitivity than Coomassie staining and eliminate organic waste disposal problems

Specifications

Applications
 
Chemiluminescence
No
Fluorescence*
Yes (stain-free imaging* and UV-excitable dyes)
*requires Bio-Rad stain-free gels
Colorimetry/densitometry
Yes
Gel documentation
Yes (Flamingo™, Oriole™, SYPRO Ruby, Coomassie, Fluorescent Orange, or Krypton Stains; ethidium bromide, GelGreen, Gel Red, SYBR® Safe, SYBR® green, or SYBR® Gold dyes; Bio-Rad stain-free gels)
Hardware Specifications
 
Maximum sample size
11.2 x 15 cm (4.4 x 6 in)
Maximum image area
11.2 x 15 cm (4.4 x 6 in)
Excitation source
Trans UVB (302 nm)
Illumination control
2 modes: Trans-UV, Trans-White
UV tray: 280-400 max 300 nm
Blue tray: 430-460, max 440 nm
White tray: visible spectra
Stain-free tray: requires Bio-Rad stain-free gels
Detector
CCD
Image resolution
1392 x 1040 pixels (1.4megapixels)
Emission filters 
Tray-based
Dynamic range
>3.0 orders of magnitude
Pixel density (gray levels)
4,096
Dynamic flat fielding
Yes
Instrument size (L x W x H)
43 x 28 x 38 cm (17 x 11 x 15 in)
Instrument weight
7.3 kg (16 lb)
Operating Ranges
 
Operating voltage
110/115/230/240 V AC nominal
Operating temperature
10–28°C (21°C recommended)
Operating humidity
<70% noncondensing
Automation Capabilities
 
Workflow automated selection
Application-driven; user-selected or recalled by a protocol
Workflow automated execution
Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
Workflow reproducibility
100% repeatability via recallable protocols, from image capture to quantitative analysis and reports
Autofocus (patent pending)
Precalibrated focus for any zoom setting or sample height
Image flat fielding**
Dynamic; precalibrated and optimized per application
**U. S. patent 5,951,838
Auto-exposure
2 user-defined modes (intense or faint bands)

Ordering

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170-8270_tn.jpg
(Discontinued) Gel Doc EZ System
1708270
Gel imaging system, includes darkroom, camera, cables, Image Lab™ software compatible with Windows or Mac OS, and stain-free imaging tray. Additional imaging trays available separately (#170-8271, 170-8272, 170-8273)
Image
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1708272

Pkg of 1, sample tray for gels stained with Coomassie Blue, copper, silver, or zinc stains, for use with Gel Doc™ EZ system; education use only

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(Discontinued) Blue Sample Tray
1708273

Pkg of 1, sample tray for gels stained with GelGreen or any SYBR® stain, for use with Gel Doc™ EZ system; education use only

Accessories

Documents

Number Description Options
10000076956
Gel Doc™ EZ Imaging System with Image Lab™ Software Instrument Guide
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
6008
Comparison of the Criterion TGX Stain-Free Precast Gel System and Standard Coomassie Staining Procedures for Running and Imaging Protein Gels
5939
Overcoming the Coomassie Blues, Rev A
5782
In-Gel Protein Quantitation Using the Criterion Stain-Free Gel Imaging System
5810
Compatibility of the Criterion Stain-Free Gel Imaging System with Mass Spectrometric Protein Analysis
6001
Rapid Validation of Purified Proteins Using Criterion Stain-Free Gels
6147
Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B