ddPCR and RT-PCR Assays for SARS-CoV-2 Variant Surveillance

As new disease variants emerge, the need for fast, sensitive, and reliable tests is essential both for tracking purposes and sequencing prioritization.

Bio-Rad's SARS-CoV-2 Variant Assays are predesigned mutation detection assays targeting the most prominent variants. These single-tube duplex assays are designed to support detection of a mutant in the FAM channel and wild-type sequence in the HEX channel, using either Droplet Digital PCR (ddPCR) or RT-PCR.

New assays will continue to be made available as new variants emerge.

The SARS-CoV-2 Variant Assays are for research use only. Not for use in diagnostic procedures.

SARS-CoV-2 Variant Assays for ddPCR™ and RT-PCR

These assays were designed specifically for Droplet Digital PCR or RT-PCR using our Expert Design engine, which is validated to create high-quality assays. The ddPCR™ assays should be lab validated using our One-Step RT-ddPCR Kit to detect RNA instead of the standard mix for DNA.

The RT-PCR assays can be used for analysis of single mutations by allelic discrimination or analysis of multiple mutations by Cq or endpoint RFU using multiplexed panels that detect 4 mutations in one well. Refer to the product info sheet to learn more about the format of these assays.

RT-PCR users can select a single assay ID for specific primer/probe set against a single variant of interest, or a specific group of variant-associated mutations. RT-PCR assays were wet-lab verified with the Reliance One-Step Multiplex Supermix and Bio-Rad’s Exact Diagnostics variant assay controls; see the assay protocols for more information.

SARS-CoV-2 Variant Assays for ddPCR™ and RT-PCR

These assays were designed specifically for Droplet Digital PCR using our Expert Design engine, which is validated to create high-quality assays. The ddPCR™ assays should be validated using our One-Step RT-ddPCR Kit to detect RNA instead of the standard mix for DNA.

All assays are compatible with RT-PCR when used by researchers as an allelic discrimination assay with our Reliance One-Step Multiplex RT-qPCR Supermix. Those assays indicated with a check mark (✓) have been wet-lab verified for RT-PCR. All assays will require validation prior to use by researchers trained in RT-PCR technology.

gBlocks from IDT are recommended for validation as positive controls for the variant targets. Positive control sequences are included on the assay details page of each duplex assay. The SARS-CoV-2 positive standard can be validated for use as a positive control for the wild-type targets (Wuhan strain). For RT-PCR assays using human samples, the Human RNAse P RPP40 Assay can be used as a sample control to monitor for the presence of amplifiable human RNA in the extracted sample.

Note: Clicking on an Assay ID will redirect you to our Digital PCR Assays ordering portal. The assays may be used for either ddPCR™ or RT-PCR as indicated. A Bio-Rad.com user login is required to order assays.

Featured ProductSARS-CoV-2 ddPCR™ and RT-PCR Assays

Single and Multiple Mutation Assays for
SARS-CoV-2 Variants

Profile mutations associated with SARS-CoV-2 variants using new
single and multiple mutation assays designed for ddPCR™ and RT-qPCR.
For research use only (RUO).

Order SARS-CoV-2 Variant Mutation Assays »

SARS-CoV-2 Variant Assay Protocols, Kits, and Reagents

SARS-CoV-2 Variant Assay Protocols

SARS-CoV-2 Assay Kits and Reagents

SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit

The Bio-Rad SARS-CoV-2 ddPCR Kit is a powerful diagnostic tool in the battle against COVID-19, with sensitivity and precision beyond that of qPCR assays.
Reliance SARS-CoV-2 RT-PCR Assay Kit

The Reliance SARS-CoV-2 RT-PCR Assay Kit is a molecular in vitro diagnostic test targeting the virus nucleocapsid N1 and N2 genes to detect the coronavirus specifically.
One-Step RT-ddPCR Advanced Kit for Probes

Use this one-step reverse transcription digital PCR supermix to achieve improved efficiency, specificity, and sensitivity with precise RNA target quantification using Droplet Digital PCR (ddPCR).
Reliance One-Step Multiplex RT-qPCR Supermix

Use Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction with challenging templates, difficult targets, or PCR inhibitors.
SARS-CoV-2 Standard

The SARS-CoV-2 Standard contains synthetic RNA transcripts of 5 SARS-CoV-2 gene targets and Human Genomic DNA.
SARS-CoV-2 Negative

The SARS-CoV-2 Negative contains genomic DNA at 75,000 copies/ml formulated in a synthetic matrix.

Custom Assays for ddPCR and RT-PCR

Choose from a large catalog of predesigned, validated ddPCR and qPCR assays or design your own custom assays.

Expert Design ddPCR assays are designed for optimal performance and manufactured to reduce false positives.
PrimePCR Assays and panels are carefully designed and validated to yield reliable qPCR data.
ddPCR Expert Design Assays

ddPCR Expert Design Assays are designed specifically for Droplet Digital PCR by the experts that developed the technology.
PrimePCR Assays and Panels

Experimentally validated real-time PCR PCR primer and probe assays for mutation detection, gene expression, copy number variation. Design your own custom assays and panels.

ddPCR and RT-PCR Assays for SARS-CoV-2 Variant Surveillance

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SARS-CoV-2 Variant Assays for ddPCR™ and RT-PCR

SARS-CoV-2 Variant Assays for ddPCR™ and RT-PCR

Note: Clicking on an Assay ID will redirect you to our Digital PCR Assays ordering portal. The assays may be used for either ddPCR™ or RT-PCR as indicated. A Bio-Rad.com user login is required to order assays.

Featured ProductSARS-CoV-2 ddPCR™ and RT-PCR Assays

Single and Multiple Mutation Assays for
SARS-CoV-2 Variants

SARS-CoV-2 Variant Assay Protocols, Kits, and Reagents

SARS-CoV-2 Variant Assay Protocols

SARS-CoV-2 Variant Detection ddPCR Assay Protocol

SARS-CoV-2 Single Mutation RT-qPCR Assay Protocol

SARS-CoV-2 Multiple Mutation RT-qPCR Assay Protocol

SARS-CoV-2 Variant Detection ddPCR Assay Protocol

SARS-CoV-2 Variant Detection RT-PCR Assay Protocol

SARS-CoV-2 Assay Kits and Reagents

SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit

Reliance SARS-CoV-2 RT-PCR Assay Kit

One-Step RT-ddPCR Advanced Kit for Probes

Reliance One-Step Multiplex RT-qPCR Supermix

SARS-CoV-2 Standard

SARS-CoV-2 Negative

Custom Assays for ddPCR and RT-PCR

ddPCR Expert Design Assays

PrimePCR Assays and Panels

More COVID-19 Surveillance Solutions

ddPCR and RT-PCR Assays for SARS-CoV-2 Variant Surveillance

Surveillance

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SARS-CoV-2 Variant Assays for ddPCR™ and RT-PCR

SARS-CoV-2 Variant Assays for ddPCR™ and RT-PCR

Note: Clicking on an Assay ID will redirect you to our Digital PCR Assays ordering portal. The assays may be used for either ddPCR™ or RT-PCR as indicated. A Bio-Rad.com user login is required to order assays.

Featured ProductSARS-CoV-2 ddPCR™ and RT-PCR Assays

Single and Multiple Mutation Assays for SARS-CoV-2 Variants

SARS-CoV-2 Variant Assay Protocols, Kits, and Reagents

SARS-CoV-2 Variant Assay Protocols

SARS-CoV-2 Assay Kits and Reagents

Custom Assays for ddPCR and RT-PCR

More COVID-19 Surveillance Solutions

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Single and Multiple Mutation Assays for
SARS-CoV-2 Variants