ddPCR and RT-PCR Assays for SARS-CoV-2 Variant Surveillance


As new disease variants emerge, the need for fast, sensitive, and reliable tests is essential both for tracking purposes and sequencing prioritization.

Bio-Rad's SARS-CoV-2 Variant Assays are predesigned mutation detection assays targeting the most prominent variants. These single-tube duplex assays are designed to support detection of a mutant in the FAM channel and wild-type sequence in the HEX channel, using either Droplet Digital PCR (ddPCR) or RT-PCR.

New assays will continue to be made available as new variants emerge.

Information Purposes

The SARS-CoV-2 Variant Assays are for research use only. Not for use in diagnostic procedures.

Featured ProductSARS-CoV-2 ddPCR™ and RT-PCR Assays

Single and Multiple Mutation Assays for
SARS-CoV-2 Variants

Profile mutations associated with SARS-CoV-2 variants using new
single and multiple mutation assays designed for ddPCR™ and RT-qPCR.
For research use only (RUO).

Order SARS-CoV-2 Variant Mutation Assays »

SARS-CoV-2 Variant Assay Protocols, Kits, and Reagents

SARS-CoV-2 Variant Assay Protocols

SARS-CoV-2 Assay Kits and Reagents

  • SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit

    SARS-CoV-2 Droplet Digital PCR (ddPCR) Kit

    The Bio-Rad SARS-CoV-2 ddPCR Kit is a powerful diagnostic tool in the battle against COVID-19, with sensitivity and precision beyond that of qPCR assays.

  • Reliance SARS-CoV-2 RT-PCR Assay Kit

    Reliance SARS-CoV-2 RT-PCR Assay Kit

    The Reliance SARS-CoV-2 RT-PCR Assay Kit is a molecular in vitro diagnostic test targeting the virus nucleocapsid N1 and N2 genes to detect the coronavirus specifically.

  • One-Step RT-ddPCR Advanced Kit for Probes

    One-Step RT-ddPCR Advanced Kit for Probes

    Use this one-step reverse transcription digital PCR supermix to achieve improved efficiency, specificity, and sensitivity with precise RNA target quantification using Droplet Digital PCR (ddPCR).

  • Reliance One-Step Multiplex RT-qPCR Supermix

    Reliance One-Step Multiplex RT-qPCR Supermix

    Use Reliance One-Step Multiplex Supermix for sensitive amplification of up to 5 targets in a single reaction with challenging templates, difficult targets, or PCR inhibitors.

  • SARS-CoV-2 Standard

    SARS-CoV-2 Standard

    The SARS-CoV-2 Standard contains synthetic RNA transcripts of 5 SARS-CoV-2 gene targets and Human Genomic DNA.

  • SARS-CoV-2 Negative

    SARS-CoV-2 Negative

    The SARS-CoV-2 Negative contains genomic DNA at 75,000 copies/ml formulated in a synthetic matrix.


Custom Assays for ddPCR and RT-PCR

Choose from a large catalog of predesigned, validated ddPCR and qPCR assays or design your own custom assays.

  • Expert Design ddPCR assays are designed for optimal performance and manufactured to reduce false positives.
  • PrimePCR Assays and panels are carefully designed and validated to yield reliable qPCR data.
  • ddPCR Expert Design Assays

    ddPCR Expert Design Assays

    ddPCR Expert Design Assays are designed specifically for Droplet Digital PCR by the experts that developed the technology.

  • PrimePCR Assays and Panels

    PrimePCR Assays and Panels

    Experimentally validated real-time PCR PCR primer and probe assays for mutation detection, gene expression, copy number variation. Design your own custom assays and panels.

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More COVID-19 Surveillance Solutions

COVID-19 surveillance is needed to better inform public health decisions. Obstacles to surveillance work include large sample numbers, supply shortages, and complex sample types. Bio-Rad offers a range of solutions for COVID-19 surveillance.

Learn More about our COVID-19 Surveillance Solutions »