Thermal Cyclers for PCR

Thermal cyclers are essential laboratory equipment for researchers who run polymerase chain reaction (PCR) for sequencing, cloning, genotyping, mutagenesis, and many other applications. Common features of Bio-Rad's thermal cyclers include Peltier effect technology for precise temperature control, and thermal gradient to easily optimize PCR assays in a single run. Instruments include the PTC Tempo, the next generation of conventional thermal cyclers, the T100™, a great choice for routine PCR, and the flexible 1000-series, which offers multiple block formats for increased throughput capabilities, and a dual 48/48-well unit that allows two independent experiments to run simultaneously.

Bio-Rad Thermal Cyclers

	Image PTC Tempo Thermal Cycler	Image C1000 Touch Thermal Cycler	Image S1000 Thermal Cycler	Image T100 Thermal Cycler
Overview	Premium performance on a modern platform. For demanding applications and multiple users.	High performance with flexibility and multiple block options. Can connect to an S1000 Thermal Cycler.	High performance with a keypad interface. Can connect to a C1000 Thermal Cycler.	Robust and economical for routine PCR with a compact footprint.
System Tour	Launch Tour	Launch Tour	Launch Tour	Launch Tour
Block formats	Faster ramping: 96-well: 0.2 ml Higher volume: 96–deep well: 0.2 and 0.5 ml Independent blocks: dual 48-well: 0.2 ml High throughput: 384-well	Independent blocks: dual 48-well: 0.2 ml Faster ramping: 96-well: 0.2 ml Higher volume: 96–deep well: 0.2 and 0.5 ml High throughput: 384-well	Independent blocks: dual 48-well: 0.2 ml Faster ramping: 96-well: 0.2 ml Higher volume: 96–deep well: 0.2 and 0.5 ml High throughput: 384-well	96-well: 0.2 ml high-profile
Recommended Sample Volume Range	96–well: 10–50 µl 96–deep well: 10–125 µl dual 48–well: 10–50 µl 384–well: 3–20 µl	dual 48–well: 10–50 µl 96–well: 10–50 µl 96–deep well: 10–125 µl 384–well: 3–30 µl	dual 48–well: 10–50 µl 96–well: 10–50 µl 96–deep well: 10–125 µl 384–well: 3–30 µl	96-well: 15–100 µl
PCR consumables	Both high and low-profile tubes and plates	Both high and low-profile tubes and plates	Both high and low-profile tubes and plates	Only high-profile tubes and plates
Real-time PCR compatibility	N/A	Upgrades to a CFX96 or CFX384 System	N/A	N/A
User interface	8" graphical touch screen	8.5" graphical touch screen	LCD panel and keypad	5.7" graphical touch screen
Performance	Accuracy: ±0.2°C Uniformity: ±0.4°C well-to-well within 10 sec Max ramp rate: up to 5°C/sec Temperature range: 4–100°C	Accuracy: ±0.2°C Uniformity: ±0.4°C well-to-well within 10 sec Max ramp rate: up to 5°C/sec Temperature range: 0–100°C	Accuracy: ±0.2°C Uniformity: ±0.4°C well-to-well within 10 sec Max ramp rate: up to 5°C/sec Temperature range: 0–100°C	Accuracy: ±0.5°C Uniformity: ±0.5°C well-to-well within 30 sec Max ramp rate: 4°C/sec Temperature range: 4–100°C
Size (W x D x H)	28 x 50 x 26 cm (11 x 20 x 10 in)	33 x 46 x 20 cm (13 x 18 x 8 in.)	33 x 46 x 20 cm (13 x 18 x 8 in.)	26 x 47 x 23 cm (10 x 18 x 9 in.)
Weight	96, Deepwell, 384: 15 kg (33 lb) 48/48: 14 kg (31 lb)	10 kg (23 lb)	10 kg (23 lb)	9 kg (20 lb)
Connectivity	BR.io, USB A 2.0, Ethernet, barcode scanner	Connect up to three S1000 Thermal Cyclers to every C1000 Touch Thermal Cycler	Connect up to three S1000 Thermal Cyclers to every C1000 Touch Thermal Cycler	N/A
Memory capacity	<100,000 protocols; unlimited with a USB drive	<1,000 protocols; unlimited with a USB drive	>1,000 protocols; unlimited with a USB drive	<500 protocols; unlimited with a USB drive
Documentation	User Manual Specifications	User Manual Specifications	User Manual Specifications	User Manual Specifications