Size Exclusion Resins

Bio-Gel P-6DG desalting gel is a spherical polyacrylamide gel that has been optimized specifically for desalting and buffer exchange. Its high gel strength, fast flow rates, low nonspecific adsoprtion, and economical swelling ratio make it ideal for routine desalting of macromolecules in laboratory and industrial applications. Bio-Gel P-6DG gel is also convenient for buffer exchange.

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Protein purification can involve challenging separations of complex mixtures that may not resolve target proteins in a single chromatographic step. Such cases may require the use of multidimensional chromatography. Multidimensional chromatography combines a series of columns, each employing different separation mechanisms, into a single separation. Most common protein purification techniques can be incorporated into a multidimensional chromatography scheme, and such schemes provide great benefit for proteins purified on a routine basis. Typically, a multidimensional chromatographic scheme consists of an affinity purification step based on binding to a protein tag, such as multiple histidine (His) or glutathione-S-transferase (GST), followed by one or more additional resolution steps using ion exchange, size exclusion, or hydroxyapatite columns. To streamline the combination of such disparate chromatographic methods, an automated chromatography system with a flexible design and versatile control software is essential.

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Chromatography System Compatibility

Bio-Scale Mini cartridges conveniently run a wide variety of chromatography media in the Profinia™ protein purification system, the BioLogic™ LP and BioLogic DuoFlow™ systems, a peristaltic pump, and other chromatography systems. For simple step elution, the cartridges can be used with a luer lock syringe. Fittings are available for connection to HPLC-and FPLC-type chromatography systems.

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