Bio-Gel P Polyacrylamide Gel

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Bio-Gel P Polyacrylamide Gels

Overview

Specifications
  Hydrated Bead Size (µm) Hydrated Bed Volume Mass Dry Gel Typical Flow Rate (cm/hr)* Molecular Weight Fractionation Range
Bio-Gel P–2, fine 45–90 3 ml/g 5–10 100–1,800
Bio-Gel P–2, extra fine <45   <10  
Bio-Gel P–4, medium 90–180 4 ml/g 15–20 800–4,000
Bio-Gel P–4, fine 45–90   10–15  
Bio-Gel P–4, extra fine <45   <10  
Bio-Gel P–6, medium 90–180 6.5 ml/g 15–20 1,000–6,000
Bio-Gel P–6, fine 45–90   10–15  
Bio-Gel P–6, extra fine <45   <10  
Bio-Gel P–6DG gel 90–180   15–20  
Bio-Gel P–10 gel, medium 90–180 7.5 ml/g 15–20 1,500–20,000
Bio-Gel P–10 gel, fine 45–90   10–15  
Bio-Gel P–30 gel, medium 90–180 9 ml/g 7–13 2,400–40,000
Bio-Gel P–30 gel, fine 45–90   6–11  
Bio-Gel P–60 gel, medium 90–180 11 ml/g 4–6 3,000–60,000
Bio-Gel P–60 gel, fine 45–90   3–5  
Bio-Gel P–100 gel, medium 90–180 12 ml/g 4–6 5,000–100,000
Bio-Gel P–100 gel, fine 45–90   3–5  
 

*Flow rates determined in a 1.5 x 70 cm column using hydrostatic pressure head-to-bed ratio of 1:1

Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylenebisacrylamide.
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Description

Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylenebisacrylamide. Bio-Gel P gels:

  • Are supplied dry and are available in several particle size ranges with molecular weight exclusion limits ranging from 100 to 100,000
  • Are extremely hydrophilic and essentially nonionic
  • Provide efficient, gentle gel filtration of sensitive compounds
  • Do not support microbial growth or leach carbohydrates (due to their synthetic composition), as dextrose and agarose gels can

Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C.

The gels are compatible with dilute organic acids, 8 M urea, chaotropic agents, detergents, and miscible organic solvents. Alcohol, up to 20% v/v, can be used to enhance the solubility of nucleotides, peptides, and tannins without altering the exclusion properties of the gels. Formamide may also be used at full strength since Bio-Gel P gels are completely swollen in this solvent.

Bio-Gel P-6DG gel, with a molecular weight exclusion limit of 6,000, is recommended specifically for desalting and buffer exchange applications. It provides rapid results with high sample recovery and minimal sample dilution. Its hydrophilic nature ensures little or no interaction between the gel and sample molecules.


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Bio-Gel P-2 Gel

1504114
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 100–1,800 MW fractionation range

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$350.00 
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Bio-Gel P-2 Gel

1504115
500 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 100–1,800 MW fractionation range

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$1,375.00 
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Bio-Gel P-2 Gel

1504118
100 g, extra fine polyacrylamide beads for size exclusion chromatography, <45 µm wet bead size, 100–1,800 MW fractionation range

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$350.00 
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Bio-Gel P-4 Gel

1504120
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 800–4,000 MW fractionation range

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$374.00 
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Bio-Gel P-4 Gel

1504124
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 800–4,000 MW fractionation range

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$417.00 
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Bio-Gel P-4 Gel

1504128
100 g, extra fine polyacrylamide beads for size exclusion chromatography, <45 µm wet bead size, 800–4,000 MW fractionation range

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$425.00 
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Bio-Gel P-6 Gel

1504130
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range

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$311.00 
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Bio-Gel P-6 Gel

1504134
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 1,000–6,000 MW fractionation range

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$350.00 
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Bio-Gel P-6DG Gel

1500738
100 g, polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range

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$330.00 
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Bio-Gel P-6DG Gel

150-0739
1 kg, fine polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range

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Bio-Gel P-10 Gel

1504140
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,500–20,000 MW fractionation range

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$398.00 
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Bio-Gel P-10 Gel

1504144
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 1,500–20,000 MW fractionation range

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$417.00 
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Bio-Gel P-30 Gel

1504150
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 2,500–40,000 MW fractionation range

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$398.00 
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Bio-Gel P-30 Gel

1504154
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 2,500–40,000 MW fractionation range

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$416.00 
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Bio-Gel P-60 Gel

1504160
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 3,000–60,000 MW fractionation range

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$435.00 
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Bio-Gel P-60 Gel

1504164
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 3,000–60,000 MW fractionation range

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$444.00 
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Bio-Gel P-100 Gel

1504170
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 5,000–100,000 MW fractionation range

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$417.00 
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Bio-Gel P-100 Gel

1504174
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 5,000–100,000 MW fractionation range

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$417.00 
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Kits & Reagents

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If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

Econo-Pac 10DG Desalting Columns

7322010
Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit

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$250.00 
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Desalting and Cartridge Cleaning Buffer Kit

6200224
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system

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$282.00 
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Profinia Desalting Purification Kit

6200238
Kit for use with the Profinia protein purification system, includes 50 ml desalting cartridge, 2 Profinia desalting buffer kits (#620-0224)

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$765.00 
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Profinia Desalting Purification Kit

6200228
Kit for use with the Profinia protein purification system, includes Profinia desalting buffer kit (#620-0224), 2 x 10 ml desalting cartridges

This product is being discontinued soon. Please contact customer service for more information.
Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 50 ml

7325314
Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

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$1,756.00 
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Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridge, 50 ml

7325312
Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

List Price: 
 
$431.00 
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Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 10 ml

7325304
Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

List Price: 
 
$441.00 
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Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridge, 5 ml

7324502
Pkg of 1, 5 ml, prepacked gel filtration column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Bio-Gel P-6 Desalting Column, 1 x 5 ml (12009238)

Documents

Number Description Options
2068 Desalting With Bio-Gel P-6DG Desalting Gel, Rev B Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
LIT174 Instruction Manual, Bio-Gel P Polyacrylamide Gel, Rev B Click to download
LIT507 Instruction Manual, Bio-Spin 6 and 30 Columns, Rev G Click to download
5539 Automated Desalting of Proteins With the Profinia Protein Purification System: Comparison to Manual Desalting by Dialysis, Rev A Click to download
5342 Automated Three-Dimensional Purification Method for Histidine-Tagged Proteins Using the BioLogic DuoFlow Maximizer Chromatography System, Rev A Click to download
LIT81 Instruction Manual, Econo-Pac® 10DG Columns Click to download
6906 Speciality Beads: Bio-Gel™ Resins Flier, Ver A Click to download

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