Bio-Gel P Polyacrylamide Gel

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Overview

Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylenebisacrylamide.
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Description

Description

Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylenebisacrylamide. Bio-Gel P gels:

  • Are supplied dry and are available in several particle size ranges with molecular weight exclusion limits ranging from 100 to 100,000
  • Are extremely hydrophilic and essentially nonionic
  • Provide efficient, gentle gel filtration of sensitive compounds
  • Do not support microbial growth or leach carbohydrates (due to their synthetic composition), as dextrose and agarose gels can

Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C.

The gels are compatible with dilute organic acids, 8 M urea, chaotropic agents, detergents, and miscible organic solvents. Alcohol, up to 20% v/v, can be used to enhance the solubility of nucleotides, peptides, and tannins without altering the exclusion properties of the gels. Formamide may also be used at full strength since Bio-Gel P gels are completely swollen in this solvent.

Bio-Gel P-6DG gel, with a molecular weight exclusion limit of 6,000, is recommended specifically for desalting and buffer exchange applications. It provides rapid results with high sample recovery and minimal sample dilution. Its hydrophilic nature ensures little or no interaction between the gel and sample molecules.

Ordering

Ordering

items

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(Discontinued) Bio-Gel P-2 Gel
1504114
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 100–1,800 MW fractionation range
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(Discontinued) Bio-Gel P-2 Gel
1504115
500 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 100–1,800 MW fractionation range
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(Discontinued) Bio-Gel P-2 Gel
1504118
100 g, extra fine polyacrylamide beads for size exclusion chromatography, <45 µm wet bead size, 100–1,800 MW fractionation range
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(Discontinued) Bio-Gel P-4 Gel
1504120
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 800–4,000 MW fractionation range
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(Discontinued) Bio-Gel P-4 Gel
1504124
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 800–4,000 MW fractionation range
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(Discontinued) Bio-Gel P-4 Gel
1504128
100 g, extra fine polyacrylamide beads for size exclusion chromatography, <45 µm wet bead size, 800–4,000 MW fractionation range
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(Discontinued) Bio-Gel P-6 Gel
1504130
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range
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(Discontinued) Bio-Gel P-6 Gel
1504134
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 1,000–6,000 MW fractionation range
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(Discontinued) Bio-Gel P-6DG Gel
1500738
100 g, polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range
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(Discontinued) Bio-Gel P-6DG Gel
150-0739
1 kg, fine polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,000–6,000 MW fractionation range
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(Discontinued) Bio-Gel P-10 Gel
1504140
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 1,500–20,000 MW fractionation range
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(Discontinued) Bio-Gel P-10 Gel
1504144
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 1,500–20,000 MW fractionation range
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(Discontinued) Bio-Gel P-30 Gel
1504150
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 2,500–40,000 MW fractionation range
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(Discontinued) Bio-Gel P-30 Gel
1504154
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 2,500–40,000 MW fractionation range
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(Discontinued) Bio-Gel P-60 Gel
1504160
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 3,000–60,000 MW fractionation range
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(Discontinued) Bio-Gel P-60 Gel
1504164
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 3,000–60,000 MW fractionation range
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(Discontinued) Bio-Gel P-100 Gel
1504170
100 g, medium polyacrylamide beads for size exclusion chromatography, 90–180 µm wet bead size, 5,000–100,000 MW fractionation range
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(Discontinued) Bio-Gel P-100 Gel
1504174
100 g, fine polyacrylamide beads for size exclusion chromatography, 45–90 µm wet bead size, 5,000–100,000 MW fractionation range

Kits & Reagents

Kits & Reagents

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7322010
Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit
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6200224
Buffers and solutions sufficient for 10 applications, for use with the Profinia protein purification system
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6200238
Kit for use with the Profinia protein purification system, includes 50 ml desalting cartridge, 2 Profinia desalting buffer kits (#620-0224)
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6200228
Kit for use with the Profinia protein purification system, includes Profinia desalting buffer kit (#620-0224), 2 x 10 ml desalting cartridges
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7325314
Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325312
Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7325304
Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems
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7324502
Pkg of 1, 5 ml, prepacked gel filtration column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

Documents

Documents

Number Description Options
2068
Desalting With Bio-Gel P-6DG Desalting Gel, Rev B
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
LIT174
Instruction Manual, Bio-Gel P Polyacrylamide Gel, Rev B
LIT507
Instruction Manual, Bio-Spin 6 and 30 Columns, Rev G
5539
Automated Desalting of Proteins With the Profinia Protein Purification System: Comparison to Manual Desalting by Dialysis, Rev A
5342
Automated Three-Dimensional Purification Method for Histidine-Tagged Proteins Using the BioLogic DuoFlow Maximizer Chromatography System, Rev A
LIT81
Instruction Manual, Econo-Pac® 10DG Columns
6906
Speciality Beads: Bio-Gel™ Resins Flier, Ver A